scholarly journals Identification and Phenotyping of Leukocytes in Bovine Bronchoalveolar Lavage Fluid

2004 ◽  
Vol 11 (4) ◽  
pp. 795-798 ◽  
Author(s):  
E. C. Soethout ◽  
K. E. Müller ◽  
A. J. M. van den Belt ◽  
V. P. M. G. Rutten
Keyword(s):  
Flow Cytometry ◽  
Bronchoalveolar Lavage ◽  
Alveolar Macrophages ◽  
Bronchoalveolar Lavage Fluid ◽  
Lavage Fluid ◽  
Light Scatter ◽  
Specific Antibodies ◽  
Dual Laser

ABSTRACT A method is proposed to identify leukocyte subpopulations in bovine bronchoalveolar lavage fluid by dual-laser flow cytometry. The technique uses several parameters, i.e., exclusion of highly autofluorescent alveolar macrophages and inclusion of leukocytes on the basis of labeling by specific antibodies and light scatter characteristics.

Cathepsin L Activity Is Increased in Alveolar Macrophages and Bronchoalveolar Lavage Fluid of Smokers

1993 ◽  
Vol 147 (6_pt_1) ◽  
pp. 1562-1568 ◽  
Author(s):  
Hideki Takahashi ◽  
Kazumi Ishidoh ◽  
Daisuke Muno ◽  
Akihiko Ohwada ◽  
Toshihiro Nukiwa ◽  
...  
Keyword(s):  
Bronchoalveolar Lavage ◽  
Alveolar Macrophages ◽  
Bronchoalveolar Lavage Fluid ◽  
Cathepsin L ◽  
Lavage Fluid

scholarly journals Effect of exogenous surfactant on Paediatric Bronchoalveolar lavage derived macrophages’ cytokine secretion

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Lyné van Rensburg ◽  
Johann M. van Zyl ◽  
Johan Smith ◽  
Pierre Goussard
Keyword(s):  
Bronchoalveolar Lavage ◽  
South African ◽  
Alveolar Macrophages ◽  
Bronchoalveolar Lavage Fluid ◽  
Lavage Fluid ◽  
Cytokine Secretion ◽  
Exogenous Surfactant ◽  
Normal Children ◽  
African Children ◽  
South African Children

Abstract Background Bronchoalveolar lavage is a useful bronchoscopy technique. However, studies in “normal” children populations are few. Furthermore, the anti-inflammatory effects of exogenous pulmonary surfactants on the bronchoalveolar cellular components are limited. Methods Thirty children, aged 3 to 14 years, underwent diagnostic bronchoscopy and bronchoalveolar lavage. Differential cytology, cytokine and chemokine measurements were performed on the fluid after exogenous surfactant exposure. The aim of the study was to investigate the potential anti-inflammatory effects of exogenous surfactants on the bronchoalveolar lavage fluid, specifically alveolar macrophages of healthy South African children. Results Alveolar macrophages were the predominant cellular population in normal children. Patients with inflammatory pneumonopathies had significantly more neutrophils. Levels of inflammatory cytokines were significantly lower after exogenous surfactant exposure. Moreover, IL-10 and IL-12 cytokine secretion increased after exogenous surfactant exposure. Conclusion This study provides the first data on bronchoalveolar lavage of healthy South African children. Bronchoalveolar lavage fluid from patients with pulmonary inflammation was characterised by neutrophilia. Finally, we propose that exogenous surfactant treatment could help alleviate inflammation in diseased states where it occurs in the tracheobronchial tree.

scholarly journals Expression of complement-related membrane proteins on lymphocytes and alveolar macrophages in bronchoalveolar lavage fluid

1998 ◽  
Vol 47 (2) ◽  
pp. 109-116
Author(s):  
Risa Nakahara ◽  
Shuhei Takemura ◽  
Hideki Onodera ◽  
Yoshihiro Kasamatsu ◽  
Nobuyuki Seto ◽  
...  
Keyword(s):  
Membrane Proteins ◽  
Bronchoalveolar Lavage ◽  
Alveolar Macrophages ◽  
Bronchoalveolar Lavage Fluid ◽  
Lavage Fluid
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