transcriptomic profiling
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2022 ◽  
Vol 21 (2) ◽  
pp. 407-421
Author(s):  
Ying-chun ZHU ◽  
Gao-peng YUAN ◽  
Sheng-feng JIA ◽  
Guo-lin AN ◽  
Wei-hua LI ◽  
...  

2022 ◽  
Author(s):  
Yanli Xiang ◽  
Thomas Sapir ◽  
Pauline Rouillard ◽  
Marina Ferrand ◽  
Jose M Jimenez-Gomez

Many biological processes follow circadian rhythmicity and are controlled by the circadian clock. Predictable environmental changes such as seasonal variation in photoperiod can modulate circadian rhythms, allowing organisms to adjust to the time of the year. Modification of circadian clocks is especially relevant in crops to enhance their cultivability in specific regions by changing their sensibility to photoperiod. In tomato, the appearance of mutations in EMPFINDLICHER IM DUNKELROTEN LICHT 1 (EID1, Solyc09g075080) and NIGHT LIGHT-INDUCIBLE AND CLOCK-REGULATED GENE 2 (LNK2, Solyc01g068560) during domestication delayed its circadian rhythms, and allowed its expansion outside its equatorial origin. Here we study how variation in circadian rhythms in tomato affects its perception of photoperiod. To do this, we create near isogenic lines carrying combinations of wild alleles of EID1 and LNK2 and perform transcriptomic profiling under two different photoperiods. We observe that EID1, but not LNK2, has a large effect on the tomato transcriptome and its response to photoperiod. This large effect of EID1 is likely a consequence of the global phase shift elicited by this gene in tomato's circadian rhythms.


Author(s):  
Zhen-Hua Chen ◽  
Si Li ◽  
Mingrui Xu ◽  
Candace C. Liu ◽  
Hongying Ye ◽  
...  

2021 ◽  
Author(s):  
Adrian Fragoso-Luna ◽  
Cristina Ayuso ◽  
Michael Eibl ◽  
Celia Munoz-Jimenez ◽  
Vladimir Benes ◽  
...  

Control of gene expression in specific tissues and/or at certain stages of development allows the study and manipulation of gene function with high precision. Site-specific genome recombination by the Flippase (FLP) and Cre enzymes has proven particularly relevant. Joint efforts of many research groups have led to the creation of efficient FLP and Cre drivers to regulate gene expression in a variety of tissues in Caenorhabditis elegans. Here, we extend this toolkit by the addition of FLP lines that drive recombination specifically in distal tip cells, the somatic gonad, coelomocytes and the epithelial P lineage. In some cases, recombination-mediated gene knockouts do not completely deplete protein levels due to persistence of long-lived proteins. To overcome this, we developed a spatiotemporally regulated degradation system for GFP fusion proteins (GFPdeg) based on FLP-mediated recombination. Using two stable nuclear pore proteins, MEL-28/ELYS and NPP-2/NUP85 as examples, we report the benefit of combining tissue-specific gene knockout and protein degradation to achieve complete protein depletion. We also demonstrate that FLP-mediated recombination can be utilized to identify nascent transcripts in a tissue of interest. We have adapted thiol(SH)-linked alkylation for the metabolic sequencing of RNA in tissue (SLAM-ITseq) for C. elegans. By focusing on a well-characterized tissue, the hypodermis, we show that the vast majority of genes identified by SLAM-ITseq are known to be expressed in this tissue, but with the added value of temporal resolution. These tools allow combining FLP activity for simultaneous gene inactivation and transcriptomic profiling, thus enabling the inquiry of gene function in various complex biological processes.


2021 ◽  
Author(s):  
Peter Pruisscher ◽  
Philipp Lehmann ◽  
Sören Nylin ◽  
Karl Gotthard ◽  
Christopher W. Wheat

Author(s):  
David Aciole Barbosa ◽  
Bruno C. Araújo ◽  
Giovana Souza Branco ◽  
Alexandre S. Simeone ◽  
Alexandre W. S. Hilsdorf ◽  
...  

2021 ◽  
pp. 100094
Author(s):  
Jared Liu ◽  
Hsin-Wen Chang ◽  
Robby Grewal ◽  
Daniel D. Cummins ◽  
Audrey Bui ◽  
...  

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