Development of Immunoglobulin M Capture Enzyme-Linked Immunosorbent Assay To Differentiate Human Flavivirus Infections Occurring in Australia

ABSTRACT We report the development of a flavivirus immunoglobulin M (IgM) capture enzyme-linked immunosorbent assay (MAC-ELISA) which improves the determination of an infecting flavivirus serotype over that by current serological methods. A panel of 165 IgM-positive sera from flavivirus patients with specific diagnostic results was tested by the flavivirus MAC-ELISA using a panel of 10 antigens. For 134 of these sera (81.2%), the highest reactivity was demonstrated against the infecting virus, which was consistent with the original diagnostic result. Specific antibody reactions inconsistent with the original diagnosis were found for six sera (3.6%). In our experience, the flavivirus-serotyping ELISA provides a rapid and accurate alternative to other serological tests, such as hemagglutination inhibition, for the specific diagnosis of flavivirus infections.

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Direct enzyme-linked immunosorbent assay that uses peroxidase-labeled antigen for determination of immunoglobulin M antibody to cytomegalovirus.

Journal of Clinical Microbiology ◽

10.1128/jcm.13.3.416-422.1981 ◽

1981 ◽

Vol 13 (3) ◽

pp. 416-422 ◽

Cited By ~ 51

Author(s):

A M van Loon ◽

F W Heessen ◽

J T van der Logt ◽

J van der Veen

Keyword(s):

Immunosorbent Assay ◽

Immunoglobulin M ◽

Enzyme Linked Immunosorbent Assay

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Comparison of enzyme-linked immunosorbent assay, hemagglutination inhibition, and passive latex agglutination for determination of rubella immune status.

Journal of Clinical Microbiology ◽

10.1128/jcm.21.1.140-142.1985 ◽

1985 ◽

Vol 21 (1) ◽

pp. 140-142 ◽

Cited By ~ 9

Author(s):

R S Steece ◽

M S Talley ◽

M R Skeels ◽

G A Lanier

Keyword(s):

Immunosorbent Assay ◽

Hemagglutination Inhibition ◽

Immune Status ◽

Latex Agglutination ◽

Enzyme Linked Immunosorbent Assay

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Immunoglobulin G- and M-specific enzyme-linked immunosorbent assay for detection of dengue antibodies

Journal of Clinical Microbiology ◽

10.1128/jcm.9.4.498-502.1979 ◽

1979 ◽

Vol 9 (4) ◽

pp. 498-502

Author(s):

D Dittmar ◽

T J Cleary ◽

A Castro

Keyword(s):

Dengue Virus ◽

Immunosorbent Assay ◽

Immunoglobulin G ◽

Hemagglutination Inhibition ◽

Dengue Infection ◽

Immunoglobulin M ◽

Enzyme Linked Immunosorbent Assay ◽

Specific Enzyme ◽

Enzyme Conjugate ◽

Inhibition Technique

An enzyme-linked immunosorbent assay for the detection of antibodies to dengue virus is described. This method correlates well with a hemagglutination inhibition technique. The enzyme-linked immunosorbent assay can also be specific for human immunoglobulin M antibodies when a mu-chain-specific antiglobulin-enzyme conjugate and fractionated serum are employed. By using this technique, dengue immunoglobulin M antibodies were demonstrated in an infant suspected of having a recent dengue infection.

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Evaluation of a Capture Screening Enzyme-Linked Immunosorbent Assay for Combined Determination of Immunoglobulin M and G Antibodies Produced during Dengue Infection

Clinical and Diagnostic Laboratory Immunology ◽

10.1128/cdli.7.5.850-852.2000 ◽

2000 ◽

Vol 7 (5) ◽

pp. 850-852 ◽

Cited By ~ 8

Author(s):

Sai Kit Lam ◽

Cheng Lan Ew ◽

Jody L. Mitchell ◽

Andrea J. Cuzzubbo ◽

Peter L. Devine

Keyword(s):

Immunosorbent Assay ◽

Dengue Infection ◽

Immunoglobulin M ◽

Enzyme Linked Immunosorbent Assay

ABSTRACT A commercially available enzyme-linked immunosorbent assay (ELISA) (PanBio Dengue Screening ELISA) that utilized both immunoglobulin M (IgM) and IgG capture in the same microtiter well for the diagnosis of dengue infection was evaluated. Sensitivity in primary and secondary dengue was 95%, while specificity was 94%.

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Seroprevalence of mycoplasmosis in broiler, layer, and native chickens in Giza, Egypt

PLoS ONE ◽

10.1371/journal.pone.0254220 ◽

2021 ◽

Vol 16 (7) ◽

pp. e0254220

Author(s):

Saeed El-Ashram ◽

Mahmoud E. Hashad ◽

G. A. Abdel-Alim ◽

Taher Abdelhamid ◽

Heba N. Deif

Keyword(s):

Immunosorbent Assay ◽

Hemagglutination Inhibition ◽

Serological Test ◽

Enzyme Linked Immunosorbent Assay ◽

Serum Samples ◽

Serological Tests ◽

Passive Hemagglutination ◽

Positive Serum ◽

Commercial Kits ◽

Elisa Data

We aimed to investigate Mycoplasma infections among chicken flocks (Ross, Lohmann and native) in Giza, Egypt, using serological tests, including the slide plate agglutination (SPA) test, hemagglutination inhibition (HI) test, and enzyme-linked immunosorbent assay (ELISA). The slide plate agglutination examination, a serological test, indicated the prevalence of Mg and Ms infections of 10.9% and 13.2%, respectively. On 91 SPA test positive serum samples for either Mg or Ms, a passive hemagglutination/hemagglutination inhibition (HI) test was performed. The SPA and HI test findings were found to be comparable. On 90 SPA test positive samples, an ELISA was performed using commercial kits for Mg and Ms serodiagnosis. According to the ELISA data, only 83.33% and 18.88% of SPA test positive samples were confirmed as positive for Ms and Mg infections, respectively. The prevalence increased to 84.44% and 77.77%, respectively, when suspected samples were deemed positive.

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