scholarly journals Development and evaluation of a capture enzyme-linked immunosorbent assay for determination of rubella immunoglobulin M using monoclonal antibodies.

1987 ◽  
Vol 25 (6) ◽  
pp. 1033-1038 ◽  
Author(s):  
I Gerna ◽  
M Zannino ◽  
M G Revello ◽  
E Petruzzelli ◽  
M Dovis
Keyword(s):  
Monoclonal Antibodies ◽  
Immunosorbent Assay ◽  
Immunoglobulin M ◽  
Enzyme Linked Immunosorbent Assay

scholarly journals Development of Immunoglobulin M Capture Enzyme-Linked Immunosorbent Assay To Differentiate Human Flavivirus Infections Occurring in Australia

2005 ◽  
Vol 12 (3) ◽  
pp. 371-374 ◽  
Author(s):  
Carmel Taylor ◽  
Russell Simmons ◽  
Ina Smith
Keyword(s):  
Immunosorbent Assay ◽  
Specific Antibody ◽  
Hemagglutination Inhibition ◽  
Immunoglobulin M ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serological Tests ◽  
Original Diagnosis ◽  
Specific Diagnosis

ABSTRACT We report the development of a flavivirus immunoglobulin M (IgM) capture enzyme-linked immunosorbent assay (MAC-ELISA) which improves the determination of an infecting flavivirus serotype over that by current serological methods. A panel of 165 IgM-positive sera from flavivirus patients with specific diagnostic results was tested by the flavivirus MAC-ELISA using a panel of 10 antigens. For 134 of these sera (81.2%), the highest reactivity was demonstrated against the infecting virus, which was consistent with the original diagnostic result. Specific antibody reactions inconsistent with the original diagnosis were found for six sera (3.6%). In our experience, the flavivirus-serotyping ELISA provides a rapid and accurate alternative to other serological tests, such as hemagglutination inhibition, for the specific diagnosis of flavivirus infections.

scholarly journals Evaluation of a Capture Screening Enzyme-Linked Immunosorbent Assay for Combined Determination of Immunoglobulin M and G Antibodies Produced during Dengue Infection

2000 ◽  
Vol 7 (5) ◽  
pp. 850-852 ◽  
Author(s):  
Sai Kit Lam ◽  
Cheng Lan Ew ◽  
Jody L. Mitchell ◽  
Andrea J. Cuzzubbo ◽  
Peter L. Devine
Keyword(s):  
Immunosorbent Assay ◽  
Dengue Infection ◽  
Immunoglobulin M ◽  
Enzyme Linked Immunosorbent Assay

ABSTRACT A commercially available enzyme-linked immunosorbent assay (ELISA) (PanBio Dengue Screening ELISA) that utilized both immunoglobulin M (IgM) and IgG capture in the same microtiter well for the diagnosis of dengue infection was evaluated. Sensitivity in primary and secondary dengue was 95%, while specificity was 94%.

scholarly journals Development of a sensitive and specific enzyme-linked immunosorbent assay for the determination of fludioxonil residues in fruit juices

2014 ◽  
Vol 6 (22) ◽  
pp. 8924-8929 ◽  
Author(s):  
Josep V. Mercader ◽  
Antonio Abad-Fuentes ◽  
Consuelo Agulló ◽  
Antonio Abad-Somovilla ◽  
Francesc A. Esteve-Turrillas
Keyword(s):  
Monoclonal Antibodies ◽  
Immunosorbent Assay ◽  
Residue Analysis ◽  
Fruit Juices ◽  
Enzyme Linked Immunosorbent Assay ◽  
Specific Enzyme ◽  
Competitive Immunoassay ◽  
Deming Regression ◽  
And Performance

The generation and performance of the first-ever described monoclonal antibodies to fludioxonil are herein reported, and a competitive immunoassay was developed and validated for residue analysis in fruit juices by comparison with GC-MS using Deming regression and Bland–Altman analyses.

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