scholarly journals Limited Diagnostic Capacities of Two Commercial Assays for the Detection of Leptospira Immunoglobulin M Antibodies in Laos

2006 ◽  
Vol 13 (10) ◽  
pp. 1166-1169 ◽  
Author(s):  
Stuart D. Blacksell ◽  
Lee Smythe ◽  
Rattanaphone Phetsouvanh ◽  
Michael Dohnt ◽  
Rudy Hartskeerl ◽  
...  
Keyword(s):  
Diagnostic Accuracy ◽  
Immunosorbent Assay ◽  
Gold Standard ◽  
Agglutination Test ◽  
Immunoglobulin M ◽  
Microscopic Agglutination Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Diagnostic Utility

ABSTRACT The diagnostic utility of immunochromatographic (Leptotek) and enzyme-linked immunosorbent assay (ELISA; Panbio) tests for the detection of Leptospira immunoglobulin M antibodies was assessed in febrile adults admitted in Vientiane, Laos. Both tests demonstrated poor diagnostic accuracy using admission serum (Leptotek sensitivity of 47.3% and specificity of 75.5%: ELISA sensitivity of 60.9% and specificity of 65.6%) compared to the Leptospira “gold standard” microscopic agglutination test.

Detection of Leptospira DNA in Patients with Aseptic Meningitis by PCR

1998 ◽  
Vol 36 (5) ◽  
pp. 1453-1455 ◽  
Author(s):  
Eliete C. Romero ◽  
Ana E. C. Billerbeck ◽  
Valéria S. Lando ◽  
Eide D. Camargo ◽  
Candida C. Souza ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
Aseptic Meningitis ◽  
Immunosorbent Assay ◽  
Agglutination Test ◽  
Immunoglobulin M ◽  
Microscopic Agglutination Test ◽  
Enzyme Linked Immunosorbent Assay

Samples of cerebrospinal fluid from 103 patients with aseptic meningitis were tested by PCR for detection of leptospires, and the results were compared with those of the microscopic agglutination test (MAT) and an enzyme-linked immunosorbent assay for detection of immunoglobulin M (ELISA-IgM). Of these samples, 39.80% were positive by PCR and 8.74 and 3.88% were positive by MAT and ELISA-IgM, respectively.

scholarly journals Evaluation of the Indirect Hemagglutination Assay for Diagnosis of Acute Leptospirosis

1998 ◽  
Vol 36 (1) ◽  
pp. 11-14 ◽  
Author(s):  
Paul N. Levett ◽  
Carol U. Whittington
Keyword(s):  
Predictive Value ◽  
Venereal Disease ◽  
Agglutination Test ◽  
Immunoglobulin M ◽  
Microscopic Agglutination Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Hemagglutination Assay ◽  
Serological Tests ◽  
Indirect Hemagglutination ◽  
Human Sera

Serology plays an important role in the diagnosis of leptospirosis. Few laboratories have the resources and expertise to perform the microscopic agglutination test. There is a need for rapid and simple serological tests which facilitate the early diagnosis of leptospirosis, while antibiotic therapy may be most effective. A commercially available indirect hemagglutination assay (IHA; MRL Diagnostics, Cypress, Calif.) was evaluated with multiple serum specimens from 107 patients being investigated for leptospirosis. By using a combination of enzyme-linked immunosorbent assay (ELISA) methods for immunoglobulin M (IgM) and IgG antibodies and the microscopic agglutination test, 54 patients were found to have leptospirosis and 53 were found not to have leptospirosis. The sensitivity of IHA for the detection of acute leptospirosis was 100%, the specificity was 94%, the positive predictive value was 95%, and the negative predictive value was 100%. IHA was negative when 13 antinuclear antibody-positive sera, 24 serum specimens from patients with syphilis, and 16 serum specimens false positive by the Venereal Disease Research Laboratory test were tested. IHA was shown to detect both IgM and IgG classes of antibodies in human sera. Serum specimens from 27 dogs investigated for leptospirosis were studied: 3 samples gave nonspecific hemagglutination, but for all remaining samples, the results of IHA and an IgM ELISA were concordant. Performance of IHA was simple, and IHA requires no specialized equipment. It represents a useful assay for laboratories which require a leptospiral diagnostic capability but lack the expertise to perform specialist investigations.

scholarly journals Measurement of immunoglobulin M (IgM), IgG, and IgA antibodies against Yersinia enterocolitica by enzyme-linked immunosorbent assay: persistence of serum antibodies during disease

1979 ◽  
Vol 9 (3) ◽  
pp. 336-341 ◽  
Author(s):  
K Granfors
Keyword(s):  
Yersinia Enterocolitica ◽  
Immunosorbent Assay ◽  
Agglutination Test ◽  
Immunoglobulin M ◽  
Enzyme Linked Immunosorbent Assay ◽  
Human Antibodies ◽  
Heat Treated ◽  
Rabbit Igg ◽  
Iga Antibodies

An enzyme-linked immunosorbent assay for the detection and quantitation of human immunoglobulin M (IgM), IgG, and IgA antibodies against Yersinia enterocolitica is described. Formalinized or heat-treated bacteria were adsorbed onto specially designed microcuvettes, and antibodies were allowed to attach to the antigen-coated cuvettes. Rabbit anti-human mu, anti-human gamma, and anti-human alpha antisera were allowed to react with human antibodies, and these class-specific anti-immunoglobulins were detected by alkaline phosphatase-labeled swine anti-rabbit IgG. A total of 423 sera were tested. The results obtained with the enzyme-linked immunosorbent assay were compared with the results of the conventional tube agglutination test. Persistence of different antibodies was studied in six patients. Antibodies of the IgM class persisted only for 1 to 3 months after onset of the disease; thus the occurence of IgM-class Yersinia antibodies in a single sample indicates a recently acquired infection. The persistence of the IgG- and IgA-class antibodies was variable and not parallel with each other. Remarkably, all three patients in which the disease was complicated with arthritis had IgA-class Yersinia antibodies at the end of the follow-up period of 9 to 14 months, and in those without arthritis the IgA-class antibodies disappeared within 3 months after onset of the disease.

Diagnostic accuracy of ELISA kits for measurement of rabies antibodies: A systematic review and meta-analysis

2021 ◽  
Author(s):  
Weiping Ling ◽  
Suhail A R Doi ◽  
Colleen L Lau ◽  
Deborah J Mills ◽  
Polychronis Kostoulas ◽  
...  
Keyword(s):  
Systematic Review ◽  
Diagnostic Accuracy ◽  
Immunosorbent Assay ◽  
Gold Standard ◽  
Meta Analysis ◽  
Inhibition Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Antibody Levels ◽  
Fluorescent Focus

Rapid fluorescent focus inhibition test (RFFIT) is considered the gold standard for measuring rabies antibody levels; however, enzyme-linked immunosorbent assay (ELISA) is cheaper, faster, and more widely available. This meta-analysis revealed that at a cut-off point of 0.5 EU/mL, ELISA has a sensitivity of 95.6% (95%CI:67.7–99.6%) and specificity of 95.4% (95%CI:67.0–99.5%).

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