Ability of Monocyte-Derived Dendritic Cells To Secrete Oxygen Radicals in Response to Formyl Peptide Receptor Family Agonists Compared to That of Myeloid and Plasmacytoid Dendritic Cells

ABSTRACT We show that human monocyte-derived dendritic cells (DC) differ considerably from freshly isolated blood-derived myeloid and plasmacytoid DC in their abilities to produce reactive oxygen species in response to different agonists to the formyl peptide receptor family and are thus poor representatives of blood DC in this field of research.

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Lactobacilli ‐induced Generation of Reactive Oxygen Species via Formyl Peptide Receptor‐1 (FPR1) Regulates Intestinal Motility in Mice

The FASEB Journal ◽

10.1096/fasebj.2019.33.1_supplement.763.1 ◽

2019 ◽

Vol 33 (S1) ◽

Author(s):

Bindu Chandrasekharan ◽

Bejan Saeedi ◽

M Ashfaqul Alam ◽

Shanthi Srinivasan ◽

Malu Tansey ◽

...

Keyword(s):

Reactive Oxygen Species ◽

Intestinal Motility ◽

Reactive Oxygen ◽

Formyl Peptide Receptor ◽

Oxygen Species ◽

Peptide Receptor ◽

Formyl Peptide

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A methodological approach to studies of desensitization of the formyl peptide receptor: Role of the read out system, reactive oxygen species and the specific agonist used to trigger neutrophils

Journal of Immunological Methods ◽

10.1016/j.jim.2009.10.011 ◽

2010 ◽

Vol 352 (1-2) ◽

pp. 45-53 ◽

Cited By ~ 17

Author(s):

Jennie Karlsson ◽

Johan Bylund ◽

Charlotta Movitz ◽

Lena Björkman ◽

Huamei Forsman ◽

...

Keyword(s):

Reactive Oxygen Species ◽

Methodological Approach ◽

Reactive Oxygen ◽

Formyl Peptide Receptor ◽

Oxygen Species ◽

Peptide Receptor ◽

Formyl Peptide

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NOX2-Dependent Reactive Oxygen Species Regulate Formyl-Peptide Receptor 1-Mediated TrkA Transactivation in SH-SY5Y Cells

Oxidative Medicine and Cellular Longevity ◽

10.1155/2019/2051235 ◽

2019 ◽

Vol 2019 ◽

pp. 1-17 ◽

Cited By ~ 1

Author(s):

Martina Castaldo ◽

Cristiana Zollo ◽

Gabriella Esposito ◽

Rosario Ammendola ◽

Fabio Cattaneo

Keyword(s):

Reactive Oxygen Species ◽

Critical Role ◽

Reactive Oxygen ◽

Cellular Migration ◽

Formyl Peptide Receptor ◽

Neurotrophin Receptor ◽

Ros Generation ◽

Oxygen Species ◽

Peptide Receptor ◽

Formyl Peptide

Several enzymes are capable of producing reactive oxygen species (ROS), but only NADPH oxidases (NOX) generate ROS as their primary and sole function. In the central nervous system, NOX2 is the major source of ROS, which play important roles in signalling and functions. NOX2 activation requires p47phox phosphorylation and membrane translocation of cytosolic subunits. We demonstrate that SH-SY5Y cells express p47phox and that the stimulation of Formyl-Peptide Receptor 1 (FPR1) by N-fMLP induces p47phox phosphorylation and NOX-dependent superoxide generation. FPR1 is a member of the G protein-coupled receptor (GPCR) family and is able to transphosphorylate several tyrosine kinase receptors (RTKs). This mechanism requires ROS as signalling intermediates and is necessary to share information within the cell. We show that N-fMLP stimulation induces the phosphorylation of cytosolic Y490, Y751, and Y785 residues of the neurotrophin receptor TrkA. These phosphotyrosines provide docking sites for signalling molecules which, in turn, activate Ras/MAPK, PI3K/Akt, and PLC-γ1/PKC intracellular cascades. N-fMLP-induced ROS generation plays a critical role in FPR1-mediated TrkA transactivation. In fact, the blockade of NOX2 functions prevents Y490, Y751, and Y785 phosphorylation, as well as the triggering of downstream signalling cascades. Moreover, we observed that FPR1 stimulation by N-fMLP also improves proliferation, cellular migration, and neurite outgrowth of SH-SY5Y cells.

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