scholarly journals Use of Pre-S Protein-Containing Hepatitis B Virus Surface Antigens and a Powerful Adjuvant To Develop an Immune Therapy for Chronic Hepatitis B Virus Infection

2011 ◽  
Vol 19 (2) ◽  
pp. 120-127 ◽  
Author(s):  
Jung Sun Yum ◽  
Byung Cheol Ahn ◽  
Hyun Jin Jo ◽  
Dong Yeon Kim ◽  
Ki Hyun Kim ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Transgenic Mice ◽  
Surface Antigen ◽  
Immune Therapy ◽  
Surface Antigens ◽  
Virus Surface ◽  
S Protein ◽  
Chronic Hepatitis B Virus ◽  
B Virus

ABSTRACTA hepatitis B virus (HBV) vaccine has been developed using a new adjuvant and HBV surface antigens produced from a CHO cell line. The purified HBV surface antigens are composed of L protein, M protein, and S protein in a mixture of 20- and 40-nm-diameter particles and filamentous forms. This HBV surface antigen, formulated with L-pampo, a proprietary adjuvant, induced 10 times more antibody than the same antigen with alum and was capable of inducing strong immune responses in three different HBV transgenic mice. In spite of the presence of a large amount of HBV antigen in the blood, no antibody against HBV surface antigen was normally detected in these transgenic mice. After immunization, the HBV antigen was also cleared from the blood.

Placebo-controlled trial of vaccination with hepatitis B virus surface antigen in hepatitis B virus transgenic mice

1997 ◽  
Vol 26 (1) ◽  
pp. 131-137 ◽  
Author(s):  
S.M. Fazle Akbar ◽  
Kazunori Kajino ◽  
Kenji Tanimoto ◽  
Kiyotaka Kurose ◽  
Toshikazu Masumoto ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Transgenic Mice ◽  
Surface Antigen ◽  
Controlled Trial ◽  
Virus Surface ◽  
Virus Surface Antigen ◽  
B Virus

scholarly journals Overcoming T Cell Tolerance to the Hepatitis B Virus Surface Antigen in Hepatitis B Virus-Transgenic Mice

2001 ◽  
Vol 166 (2) ◽  
pp. 1389-1397 ◽  
Author(s):  
Alessandro D. Sette ◽  
Carla Oseroff ◽  
John Sidney ◽  
Jeff Alexander ◽  
Robert W. Chesnut ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
T Cell ◽  
Hepatitis B ◽  
Transgenic Mice ◽  
Surface Antigen ◽  
T Cell Tolerance ◽  
Cell Tolerance ◽  
Virus Surface ◽  
Virus Surface Antigen ◽  
B Virus

scholarly journals Phospholipid interactions of the putative fusion peptide of hepatitis B virus surface antigen S protein

1995 ◽  
Vol 76 (2) ◽  
pp. 301-308 ◽  
Author(s):  
I. Rodriguez-Crespo ◽  
E. Nunez ◽  
J. Gomez-Gutierrez ◽  
B. Yelamos ◽  
J. P. Albar ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Surface Antigen ◽  
Fusion Peptide ◽  
Virus Surface ◽  
S Protein ◽  
Virus Surface Antigen ◽  
B Virus

Inhibition of hepatitis b virus surface antigen gene expression in carcinogen-induced liver tumors from transgenic mice

1994 ◽  
Vol 9 (4) ◽  
pp. 185-192 ◽  
Author(s):  
Hend Farza ◽  
Tommaso A. Dragani ◽  
Thomas Metzler ◽  
Giacomo Manenti ◽  
Pierre Tiollais ◽  
...  
Keyword(s):  
Gene Expression ◽  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Transgenic Mice ◽  
Surface Antigen ◽  
Liver Tumors ◽  
Virus Surface ◽  
Virus Surface Antigen ◽  
Antigen Gene ◽  
B Virus

scholarly journals Demethylation by 5-Azacytidine Results in the Expression of Hepatitis B Virus Surface Antigen in Transgenic Mice

1989 ◽  
Vol 80 (4) ◽  
pp. 295-298 ◽  
Author(s):  
Kimi Araki ◽  
Jun-ichi Miyazaki ◽  
Toshiki Tsurimoto ◽  
Takeaki Inomoto ◽  
Tomohisa Iwanaga ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Transgenic Mice ◽  
Surface Antigen ◽  
Virus Surface ◽  
Virus Surface Antigen ◽  
B Virus

scholarly journals Comparison of Three Luminescent Immunoassays for Hepatitis B Virus Surface Antigen Quantification during the Natural History of Chronic Hepatitis B Virus Infection

2014 ◽  
Vol 21 (11) ◽  
pp. 1521-1527 ◽  
Author(s):  
Xiao-Dong Cheng ◽  
Liu-Wei Song ◽  
Lin-Lin Fang ◽  
Lin Yang ◽  
Yong Wu ◽  
...  
Keyword(s):  
Chronic Hepatitis ◽  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Chronic Hepatitis B ◽  
Surface Antigen ◽  
Hepatitis B Surface Antigen ◽  
Serum Samples ◽  
Virus Surface ◽  
Chronic Hepatitis B Virus ◽  
B Virus

ABSTRACTHepatitis B surface antigen (HBsAg) quantification has garnered attention because of its high predictive value in determining treatment responses. The HBsAg quantification assays, such as Architect and Elecsys, are commercially available, and more assays are in development. We aimed to compare the results of the Architect and Elecsys assays with those of a new assay, WTultra. The WTultra HBsAg assay is a sandwich chemiluminescent microplate enzyme immunoassay and provides an alternative choice which is more cost-effective and potentially applicable in developing or resource-constrained countries and areas. A total of 411 serum samples were collected from patients during various phases of chronic hepatitis B (CHB) infection. The samples were assessed using the three assays, and the results were compared and analyzed. The results for the Architect, Elecsys, and WTultra assays were well correlated according to the overall results for the samples (correlation coefficients,rArchitect versus WTultra= 0.936,rArchitect versus Elecsys= 0.952, andrWTultra versus Elecsys= 0.981) and the various infection phases (rArchitect versus WTultraranging from 0.67 to 0.975,rArchitect versus Elecsysranging from 0.695 to 0.982, andrWTultra versus Elecsysranging from 0.877 to 0.99). Additionally, consistent results were observed according to genotype (genotype B:rArchitect versus WTultra= 0.976,rArchitect versus Elecsys= 0.978, andrWTultra versus Elecsys= 0.979; genotype C:rArchitect versus WTultra= 0.950,rArchitect versus Elecsys= 0.963, andrWTultra versus Elecsys= 0.981) and hepatitis B virus (HBV) DNA levels (rArchitect= 0.540,rWTultra= 0.553, andrElecsys= 0.580). In conclusion, the Elecsys and WTultra assays were well correlated with the Architect assay, irrespective of the CHB infection phase or genotype. All of these assays are reliable for HBsAg quantification.

scholarly journals Upconverting nanoparticle reporter–based highly sensitive rapid lateral flow immunoassay for hepatitis B virus surface antigen

2020 ◽  
Author(s):  
Iida Martiskainen ◽  
Sheikh M. Talha ◽  
Karoliina Vuorenpää ◽  
Teppo Salminen ◽  
Etvi Juntunen ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Surface Antigen ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Virus Surface ◽  
Virus Surface Antigen ◽  
Chronic Hepatitis B Virus ◽  
B Virus ◽  
Blood Screening

AbstractDetection of hepatitis B Virus surface antigen (HBsAg) is an established method for diagnosing both acute and chronic hepatitis B virus (HBV) infection. In addition to enzyme immunoassays (EIAs), rapid diagnostic tests (RDTs) are available for the detection of HBsAg in resource-poor settings. However, the available RDTs have inadequate sensitivity and therefore are not suitable for diagnosis of patients with low levels of HBsAg and for blood screening. To provide a high-sensitivity RDT, we developed a lateral flow immunoassay (LFIA) for HBsAg utilizing upconverting nanoparticle (UCNP) reporter. The UCNP-LFIA can use whole blood, serum, or plasma and the results can be read in 30 min using a reader device. When compared with a commercial conventional visually read LFIA, the developed UCNP-LFIA had a Limit of Detection (LoD) of 0.1 IU HBsAg/ml in spiked serum, whereas the LoD of the conventional LFIA was 3.2 IU HBsAg/ml. The developed UCNP-LFIA fulfills the WHO criterion for blood screening (LoD ≤ 0.13 IU HBsAg/ml) in terms of LoD. The UCNP-LFIA and conventional LFIA were evaluated with well-characterized sample panels. The UCNP-LFIA detected 20/24 HBsAg-positive samples within the HBsAg Performance Panel and 8/10 samples within the Mixed Titer Performance Panel, whereas the conventional LFIA detected 8/24 and 4/10 samples in these panels, respectively. The performance of the assays was further evaluated with HBsAg-positive (n = 108) and HBsAg-negative (n = 315) patient samples. In comparison with a central laboratory test, UCNP-LFIA showed 95.4% (95% CI: 89.5–98.5%) sensitivity whereas sensitivity of the conventional LFIA was 87.7% (95%CI: 79.9–93.3%).

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