scholarly journals Characterization of Fifty Putative Inclusion Membrane Proteins Encoded in the Chlamydia trachomatis Genome

2008 ◽  
Vol 76 (6) ◽  
pp. 2746-2757 ◽  
Author(s):  
Zhongyu Li ◽  
Chaoqun Chen ◽  
Ding Chen ◽  
Yimou Wu ◽  
Youmin Zhong ◽  
...  
Keyword(s):  
Chlamydia Trachomatis ◽  
Membrane Proteins ◽  
Chlamydial Infection ◽  
Distribution Patterns ◽  
Western Blot Assay ◽  
Inclusion Membrane ◽  
Urogenital Infection ◽  
Infected Cells ◽  
Inclusion Membrane Proteins

ABSTRACT Although the Chlamydia trachomatis genome is predicted to encode 50 inclusion membrane proteins, only 18 have been experimentally localized in the inclusion membrane of C. trachomatis-infected cells. Using fusion proteins and anti-fusion protein antibodies, we have systematically evaluated all 50 putative inclusion membrane proteins for their localization in the infected cells, distribution patterns, and effects on subsequent chlamydial infection when expressed ectopically, as well as their immunogenicity during chlamydial infection in humans. Twenty-two of the 50 proteins were localized in the inclusion membrane, and 7 were detected inside the inclusions, while the location of the remaining 21 was not defined. Four (CT225, CT228, CT358, and CT440) of the 22 inclusion membrane-localized proteins were visualized in the inclusion membrane of Chlamydia-infected cells for the first time in the current study. The seven intra-inclusion-localized proteins were confirmed to be chlamydial organism proteins in a Western blot assay. Further characterization of the 50 proteins revealed that neither colocalization with host cell endoplasmic reticulum nor inhibition of subsequent chlamydial infection by ectopically expressed proteins correlated with the inclusion membrane localization. Interestingly, antibodies from women with C. trachomatis urogenital infection preferentially recognized proteins localized in the inclusion membrane, and the immunodominant regions were further mapped to the region predicted to be on the cytoplasmic side of the inclusion membrane. These observations suggest that most of the inclusion membrane-localized proteins are both expressed and immunogenic during C. trachomatis infection in humans and that the cytoplasmic exposure may enhance the immunogenicity.

Identification and characterization of a Chlamydia trachomatis early operon encoding four novel inclusion membrane proteins

1999 ◽  
Vol 33 (4) ◽  
pp. 753-765 ◽  
Author(s):  
Marci A. Scidmore-Carlson ◽  
Edward I. Shaw ◽  
Cheryl A. Dooley ◽  
Elizabeth R. Fischer ◽  
Ted Hackstadt
Keyword(s):  
Chlamydia Trachomatis ◽  
Membrane Proteins ◽  
Inclusion Membrane ◽  
Inclusion Membrane Proteins ◽  
Identification And Characterization

scholarly journals Characterization of interactions between inclusion membrane proteins from Chlamydia trachomatis

2015 ◽  
Vol 5 ◽  
Author(s):  
Emilie Gauliard ◽  
Scot P. Ouellette ◽  
Kelsey J. Rueden ◽  
Daniel Ladant
Keyword(s):  
Chlamydia Trachomatis ◽  
Membrane Proteins ◽  
Inclusion Membrane ◽  
Inclusion Membrane Proteins

scholarly journals Absence of Specific Chlamydia trachomatis Inclusion Membrane Proteins Triggers Premature Inclusion Membrane Lysis and Host Cell Death

Cell Reports ◽  
2017 ◽  
Vol 19 (7) ◽  
pp. 1406-1417 ◽  
Author(s):  
Mary M. Weber ◽  
Jennifer L. Lam ◽  
Cheryl A. Dooley ◽  
Nicholas F. Noriea ◽  
Bryan T. Hansen ◽  
...  
Keyword(s):  
Cell Death ◽  
Chlamydia Trachomatis ◽  
Membrane Proteins ◽  
Host Cell ◽  
Inclusion Membrane ◽  
Host Cell Death ◽  
Membrane Lysis ◽  
Inclusion Membrane Proteins

scholarly journals The Hypothetical Protein CT813 Is Localized in the Chlamydia trachomatis Inclusion Membrane and Is Immunogenic in Women Urogenitally Infected with C. trachomatis

2006 ◽  
Vol 74 (8) ◽  
pp. 4826-4840 ◽  
Author(s):  
Chaoqun Chen ◽  
Ding Chen ◽  
Jyotika Sharma ◽  
Wen Cheng ◽  
Youmin Zhong ◽  
...  
Keyword(s):  
Chlamydia Trachomatis ◽  
Host Cell ◽  
Fusion Proteins ◽  
Chlamydial Infection ◽  
Natural Infection ◽  
Hypothetical Protein ◽  
Growth Cycle ◽  
Host Cells ◽  
Inclusion Membrane ◽  
Urogenital Infection

ABSTRACT Using antibodies raised with chlamydial fusion proteins, we have localized a protein encoded by hypothetical open reading frame CT813 in the inclusion membrane of Chlamydia trachomatis. The detection of the C. trachomatis inclusion membrane by an anti-CT813 antibody was blocked by the CT813 protein but not unrelated fusion proteins. The CT813 protein was detected as early as 12 h after chlamydial infection and was present in the inclusion membrane during the entire growth cycle. All tested serovars from C. trachomatis but not other chlamydial species expressed the CT813 protein. Exogenously expressed CT813 protein in HeLa cells displayed a cytoskeleton-like structure similar to but not overlapping with host cell intermediate filaments, suggesting that the CT813 protein is able to either polymerize or associate with host cell cytoskeletal structures. Finally, women with C. trachomatis urogenital infection developed high titers of antibodies to the CT813 protein, demonstrating that the CT813 protein is not only expressed but also immunogenic during chlamydial infection in humans. In all, the CT813 protein is an inclusion membrane protein unique to C. trachomatis species and has the potential to interact with host cells and induce host immune responses during natural infection. Thus, the CT813 protein may represent an important candidate for understanding C. trachomatis pathogenesis and developing intervention and prevention strategies for controlling C. trachomatis infection.

scholarly journals Expression and Localization of Predicted Inclusion Membrane Proteins in Chlamydia trachomatis

2015 ◽  
Vol 83 (12) ◽  
pp. 4710-4718 ◽  
Author(s):  
Mary M. Weber ◽  
Laura D. Bauler ◽  
Jennifer Lam ◽  
Ted Hackstadt
Keyword(s):  
Chlamydia Trachomatis ◽  
Membrane Proteins ◽  
Membrane Microdomains ◽  
Membrane Expression ◽  
Hydrophobic Domain ◽  
Inclusion Membrane ◽  
Content Type ◽  
Genetic Tools ◽  
Type Iii ◽  
Inclusion Membrane Proteins

Chlamydia trachomatisis an obligate intracellular pathogen that replicates in a membrane-bound vacuole termed the inclusion. Early in the infection cycle, the pathogen extensively modifies the inclusion membrane through incorporation of numerous type III secreted effector proteins, called inclusion membrane proteins (Incs). These proteins are characterized by a bilobed hydrophobic domain of 40 amino acids. The presence of this domain has been used to predict up to 59 putative Incs forC. trachomatis; however, localization to the inclusion membrane with specific antibodies has been demonstrated for only about half of them. Here, we employed recently developed genetic tools to verify the localization of predicted Incs that had not been previously localized to the inclusion membrane. Expression of epitope-tagged putative Incs identified 10 that were previously unverified as inclusion membrane localized and thus authentic Incs. One novel Inc and 3 previously described Incs were localized to inclusion membrane microdomains, as evidenced by colocalization with phosphorylated Src (p-Src). Several predicted Incs did not localize to the inclusion membrane but instead remained associated with the bacteria. UsingYersiniaas a surrogate host, we demonstrated that many of these are not secreted via type III secretion, further suggesting they may not be true Incs. Collectively, our results highlight the utility of genetic tools for demonstrating secretion from chlamydia. Further mechanistic studies aimed at elucidating effector function will advance our understanding of how the pathogen maintains its unique intracellular niche and mediates interactions with the host.

scholarly journals Characterization of Outer Membrane Proteins in Chlamydia trachomatis LGV Serovar L2

2001 ◽  
Vol 183 (8) ◽  
pp. 2686-2690 ◽  
Author(s):  
Regina J. Tanzer ◽  
Thomas P. Hatch
Keyword(s):  
Chlamydia Trachomatis ◽  
Membrane Proteins ◽  
Membrane Protein ◽  
Outer Membrane ◽  
Outer Membrane Protein ◽  
Outer Membrane Proteins ◽  
Major Outer Membrane Protein ◽  
Developmental Cycle ◽  
Reading Frame

ABSTRACT We used a photoactivatable, lipophilic reagent, 3′-(trifluoromethyl)-3-(m-[125I]iodophenyl)diazirine, to label proteins in the outer membrane of elementary bodies ofChlamydia trachomatis LGV serovar L2 and mass spectrometry to identify the labeled proteins. The identified proteins were polymorphic outer membrane proteins E, G, and H, which were made late in the developmental cycle, the major outer membrane protein, and a mixture of 46-kDa proteins consisting of the open reading frame 623 protein and possibly a modified form of the major outer membrane protein.

Inclusion membrane proteins of Chlamydiaceae

2008 ◽  
Vol 2 (2) ◽  
pp. 148-159 ◽  
Author(s):  
E. S. Kostryukova ◽  
V. N. Lazarev ◽  
V. M. Govorum
Keyword(s):  
Membrane Proteins ◽  
Inclusion Membrane ◽  
Inclusion Membrane Proteins
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