scholarly journals Assay of type-specific and type-common antibodies to herpes simplex virus types 1 and 2 in human sera.

1981 ◽  
Vol 31 (3) ◽  
pp. 1062-1070 ◽  
Author(s):  
R Eberle ◽  
R J Courtney
Keyword(s):  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Human Sera ◽  
Simplex Virus

Herpes Simplex Virus Nuclear Nonvirion Antigens Detected by Anticomplement Immunofluorescence

1976 ◽  
Vol 62 (6) ◽  
pp. 609-614 ◽  
Author(s):  
Giulio Tarro ◽  
Giovan Giacomo Giordano ◽  
Armando Tripodi ◽  
Romolo Cerra ◽  
Mario Di Gioia ◽  
...  
Keyword(s):  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Viral Genome ◽  
Squamous Cell Carcinomas ◽  
Nuclear Antigen ◽  
New Approach ◽  
Human Sera ◽  
Simplex Virus ◽  
And Control

The finding of a nuclear antigen by anticomplement immunofluorescence in cells treated with cytosine-arabinoside after infection of Herpes Simplex Virus (HSV), opens a new approach to the problem of the role of this virus in certain human cancers. Complement-fixing tests of HSV markers with cancer and control human sera as well as with hyperimmune guinea pig antisera are discussed, suggesting another parameter for studies of squamous cell carcinomas. The finding of HSV antigens in selected tumors as the expression of repressed viral genome proves a continuing release of virus specific message and supports the important role of the virus in the development of the tumor.

scholarly journals Comparison of antibody-dependent cellular cytotoxicity and complement-dependent antibody lysis of herpes simplex virus-infected cells as methods of detecting antiviral antibodies in human sera

1977 ◽  
Vol 5 (6) ◽  
pp. 551-558
Author(s):  
T Subramanian ◽  
W E Rawls
Keyword(s):  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Cellular Cytotoxicity ◽  
Antibody Dependent Cellular Cytotoxicity ◽  
Effector Cells ◽  
Herpes Simplex Viruses ◽  
Adcc Assay ◽  
Human Sera ◽  
Infected Cells ◽  
Simplex Virus

An antibody-dependent cellular cytotoxicity (ADCC) assay was used to detect antibodies to the herpes simplex viruses in humans sera. The assay utilized the release of 51Cr from BHK-21 cells infected with the viruses, hamster peritoneal exudate cells as effector cells, and antiviral antibodies in human sera. The technique was found to be far more sensitive than complement-dependent antibody lysis of infected cells and virus neutralization. The ADCC assay was useful in detecting antibodies in sera that had low titers or no antibodies detectable by other methods. In a sample of 100 sera from university students, 40 were positive by complement-dependent lysis whereas 73 were positive by ADCC. Of 400 sera from women with cervical cancer, 17 did not have detectable antibodies by microneutralization or complement-dependent lysis; however, all sera were positive by ADCC, suggesting that all of the women had been infected in the past with one or both types of herpes simplex virus.

Sign in / Sign up

Export Citation Format

Share Document