scholarly journals Ingestion and intracellular survival of Brucella abortus in human and bovine polymorphonuclear leukocytes.

1984 ◽  
Vol 46 (1) ◽  
pp. 224-230 ◽  
Author(s):  
L K Riley ◽  
D C Robertson
Keyword(s):  
Brucella Abortus ◽  
Polymorphonuclear Leukocytes ◽  
Intracellular Survival

scholarly journals Zinc Uptake System (znuA Locus) of Brucella abortus is Essential for Intracellular Survival and Virulence in Mice

2004 ◽  
Vol 66 (9) ◽  
pp. 1059-1063 ◽  
Author(s):  
Suk KIM ◽  
Kenta WATANABE ◽  
Toshikazu SHIRAHATA ◽  
Masahisa WATARAI
Keyword(s):  
Brucella Abortus ◽  
Intracellular Survival ◽  
Zinc Uptake ◽  
Uptake System

scholarly journals Brucella abortus Rough Mutants Are Cytopathic for Macrophages in Culture

2004 ◽  
Vol 72 (1) ◽  
pp. 440-450 ◽  
Author(s):  
Jianwu Pei ◽  
Thomas A. Ficht
Keyword(s):  
Brucella Abortus ◽  
Cytopathic Effect ◽  
Apoptotic Cell ◽  
Morphological Changes ◽  
Annexin V ◽  
Intracellular Survival ◽  
Intracellular Killing ◽  
Vitro Assay ◽  
O Antigen

ABSTRACT Rough mutants of Brucella spp. are attenuated for survival in animal models. However, conflicting in vitro evidence has been obtained concerning the intracellular survival of rough mutants. Transposon-derived rough mutants isolated in our laboratory were previously shown to exhibit small but significant reductions in intracellular survival in a 12-h in vitro assay. Several recent publications report that rough mutants exhibited increased macrophage uptake relative to their smooth parental strains, and a reduction in numbers at the end of the assay has been interpreted as intracellular killing. In an effort to explore the role of O antigen in the interaction between Brucella abortus and macrophages, we have monitored the uptake of rough mutants and survival in vitro by using the murine macrophage cell line J774.A1. The results confirm a 10- to 20-fold-increased uptake of rough mutants over that of smooth organisms under standard conditions. Recovery of the rough mutants persisted up to 8 h postinfection, but at the point when intracellular replication of the smooth organisms was observed, the number of rough organisms recovered declined. Fluorescence microscopy revealed the intracellular multiplication of both smooth and rough organisms, and assays performed in the absence of antibiotic confirmed the replication of the rough organisms. Examination by phase-contrast microscopy revealed the lytic death of macrophages infected with the rough mutants, which was confirmed by the release of lactate dehydrogenase (LDH) from the cell cytoplasm. Thus, the decline in the number of rough organisms was the result of the lysis of macrophages and not from intracellular killing. The cytopathic effect is characterized as necrotic rather than apoptotic cell death based on early LDH release, annexin V and propidium iodide staining, morphological changes of infected cells and nuclei, and glycine protection. The cytopathic effect was observed with macrophages at multiplicities of infection (MOIs) of as low as 20 and was not observed with epithelial cells at MOIs of as high as 2000. These findings suggest a role for O antigen during the early stages of host-agent interaction that is essential in establishing an intracellular niche that maintains and supports persistent intracellular infection resulting in disease.

The role of TREM-2 in internalization and intracellular survival of Brucella abortus in murine macrophages

2015 ◽  
Vol 163 (3-4) ◽  
pp. 194-201 ◽  
Author(s):  
Pan Wei ◽  
Qiang Lu ◽  
Guimei Cui ◽  
Zhenhong Guan ◽  
Li Yang ◽  
...  
Keyword(s):  
Brucella Abortus ◽  
Intracellular Survival ◽  
Murine Macrophages
Sign in / Sign up

Export Citation Format

Share Document