scholarly journals Genetic analysis of virulence plasmid from a serogroup 9 Yersinia enterocolitica strain: role of outer membrane protein P1 in resistance to human serum and autoagglutination.

1985 ◽  
Vol 48 (3) ◽  
pp. 782-786 ◽  
Author(s):  
G Balligand ◽  
Y Laroche ◽  
G Cornelis
Keyword(s):  
Membrane Protein ◽  
Genetic Analysis ◽  
Human Serum ◽  
Outer Membrane ◽  
Yersinia Enterocolitica ◽  
Outer Membrane Protein ◽  
Virulence Plasmid ◽  
Enterocolitica Strain

Differences in Heat Resistance among Pathogenic Yersinia enterocolitica Depended on Growth Temperature and Serotype

2005 ◽  
Vol 68 (5) ◽  
pp. 1081-1082 ◽  
Author(s):  
H. HAYASHIDANI ◽  
Y. HARA-KUDO ◽  
S. KINOSHITA ◽  
K. SAEKI ◽  
A. T. OKATANI ◽  
...  
Keyword(s):  
Membrane Protein ◽  
Heat Resistance ◽  
Outer Membrane ◽  
Yersinia Enterocolitica ◽  
Outer Membrane Protein ◽  
Growth Temperature ◽  
Virulence Plasmid ◽  
Temperature Dependent ◽  
Virulence Plasmids ◽  
Pathogenic Yersinia

To gain a better understanding about the effect of growth temperature on heat resistance of Yersinia enterocolitica, we determined decimal reduction times at 60°C (D60-values) for O:3; O:5,27; O:8; and O:9 strains harboring virulence plasmid coding for Yersinia outer membrane protein and experimentally virulence plasmid–deleted strains after they were grown to stationary phase at 7, 25, or 37°C. Bacteria were inoculated into Trypticase soy broth and were incubated at several temperatures. D60-values of O:3; O:5,27; and O:8 strains were larger when they were grown at 37°C than at 7 or 25°C, despite the presence or absence of virulence plasmids. However, similar D60-values were observed in O:9 strains, despite growth at 7, 25, or 37°C. The results indicate two types of Y. enterocolitica strains, growth temperature–dependent and –independent, and a Yersinia outer membrane protein that is not directly involved in growth temperature–dependent heat resistance.

scholarly journals Immunoglobulin G antibodies directed against protein III block killing of serum-resistant Neisseria gonorrhoeae by immune serum.

1986 ◽  
Vol 164 (5) ◽  
pp. 1735-1748 ◽  
Author(s):  
P A Rice ◽  
H E Vayo ◽  
M R Tam ◽  
M S Blake
Keyword(s):  
Membrane Protein ◽  
Neisseria Gonorrhoeae ◽  
Human Serum ◽  
Outer Membrane ◽  
Outer Membrane Protein ◽  
Immune Serum ◽  
Igg Antibodies ◽  
Porin Protein ◽  
Blocking Activity ◽  
Normal Human

Neisseria gonorrhoeae that resist complement-dependent killing by normal human serum (NHS) are sometimes killed by immune convalescent serum from patients recovering from disseminated gonococcal infection (DGI). In these studies, killing by immune serum was prevented or blocked by IgG isolated from NHS. Purified human IgG antibodies directed against gonococcal protein III, an antigenically conserved outer membrane protein, contained most of the blocking activity in IgG. Antibodies specific for gonococcal porin (protein I), the major outer membrane protein, displayed no blocking function. In separate experiments, immune convalescent DGI serum which did not exhibit bactericidal activity was restored to killing by selective depletion of protein III antibodies by immunoabsorption. These studies indicate that protein III antibodies in normal and immune human serum play a role in serum resistance of N. gonorrhoeae.

Sign in / Sign up

Export Citation Format

Share Document