scholarly journals Humoral Immunity to Commensal Oral Bacteria in Human Infants: Salivary Secretory Immunoglobulin A Antibodies Reactive withStreptococcus mitis biovar 1, Streptococcus oralis, Streptococcus mutans, andEnterococcus faecalis during the First Two Years of Life

1999 ◽  
Vol 67 (4) ◽  
pp. 1878-1886 ◽  
Author(s):  
Michael F. Cole ◽  
Stacey Bryan ◽  
Mishell K. Evans ◽  
Cheryl L. Pearce ◽  
Michael J. Sheridan ◽  
...  
Keyword(s):  
Streptococcus Mutans ◽  
Immunoglobulin A ◽  
Oral Bacteria ◽  
Secretory Immunoglobulin A ◽  
Oral Streptococci ◽  
Human Infants ◽  
Whole Cells ◽  
Streptococcus Oralis ◽  
Salivary Secretory Immunoglobulin A ◽  
Secretory Immunoglobulin

ABSTRACT Secretory immunoglobulin A (SIgA) antibodies reactive with the pioneer oral streptococci Streptococcus mitis biovar 1 andStreptococcus oralis, the late oral colonizerStreptococcus mutans, and the pioneer enteric bacteriumEnterococcus faecalis in saliva samples from 10 human infants from birth to age 2 years were analyzed. Low levels of salivary SIgA1 and SIgA2 antibodies reactive with whole cells of all four species were detected within the first month after birth, even thoughS. mutans and E. faecalis were not recovered from the mouths of the infants during the study period. Although there was a fivefold increase in the concentration of SIgA between birth and age 2 years, there were no differences between the concentrations of SIgA1 and SIgA2 antibodies reactive with the four species over this time period. When the concentrations of SIgA1 and SIgA2 antibodies reactive with all four species were normalized to the concentrations of SIgA1 and SIgA2 in saliva, SIgA1 and SIgA2 antibodies reactive with these bacteria showed a significant decrease from birth to 2 years of age. Adsorption of each infant’s saliva with cells of one species produced a dramatic reduction of antibodies recognizing the other three species. Sequential adsorption of saliva samples removed all SIgA antibody to the bacteria, indicating that the SIgA antibodies were directed to antigens shared by all four species. The induction by the host of a limited immune response to common antigens that are likely not involved in adherence may be among the mechanisms that commensal streptococci employ to persist in the oral cavity.

scholarly journals Study of Humoral Immunity to Commensal Oral Bacteria in Human Infants Demonstrates the Presence of Secretory Immunoglobulin A Antibodies Reactive with Actinomyces naeslundii Genospecies 1 and 2 Ribotypes

2004 ◽  
Vol 11 (3) ◽  
pp. 473-482 ◽  
Author(s):  
Michael F. Cole ◽  
Mishell K. Evans ◽  
Jennifer L. Kirchherr ◽  
Michael J. Sheridan ◽  
G. H. W. Bowden
Keyword(s):  
Cell Wall ◽  
Immunoglobulin A ◽  
Oral Bacteria ◽  
Enzyme Linked Immunosorbent Assay ◽  
Secretory Immunoglobulin A ◽  
Cell Wall Proteins ◽  
Human Infants ◽  
Specific Antibodies ◽  
Actinomyces Naeslundii ◽  
Secretory Immunoglobulin

ABSTRACT The mouths of three human infants were examined from birth to age 2 years to detect colonization of Actinomyces naeslundii genospecies 1 and 2. These bacteria did not colonize until after tooth eruption. The diversity of posteruption isolates was determined by ribotyping. Using immunoblotting and enzyme-linked immunosorbent assay, we determined the reactivity of secretory immunoglobulin A (SIgA) antibodies in saliva samples collected from each infant before and after colonization against cell wall proteins from their own A. naeslundii strains and carbohydrates from standard A. naeslundii genospecies 1 and 2 strains. A. naeslundii genospecies 1 and 2 carbohydrate-reactive SIgA antibodies were not detected in any saliva sample. However, SIgA antibodies reactive with cell wall proteins were present in saliva before these bacteria colonized the mouth. These antibodies could be almost completely removed by absorption with A. odontolyticus, a species known to colonize the human mouth shortly after birth. However, after colonization by A. naeslundii genospecies 1 and 2, specific antibodies were induced that could not be removed by absorption with A. odontolyticus. Cluster analysis of the patterns of reactivity of postcolonization salivary antibodies from each infant with antigens from their own strains showed that not only could these antibodies discriminate among strains but antibodies in saliva samples collected at different times showed different reactivity patterns. Overall, these data suggest that, although much of the salivary SIgA antibodies reactive with A. naeslundii genospecies 1 and 2 are directed against genus-specific or more broadly cross-reactive antigens, species, genospecies, and possibly strain-specific antibodies are induced in response to colonization.

Prematch Salivary Secretory Immunoglobulin A in Soccer Players From the 2014 World Cup Qualifying Campaign

2015 ◽  
Vol 10 (3) ◽  
pp. 401-403 ◽  
Author(s):  
Ryland Morgans ◽  
Adam Owen ◽  
Dominic Doran ◽  
Barry Drust ◽  
James P. Morton
Keyword(s):  
Fluid Collection ◽  
Immunoglobulin A ◽  
Soccer Players ◽  
Repeated Measure ◽  
Training Period ◽  
Secretory Immunoglobulin A ◽  
Short Term ◽  
World Cup ◽  
Salivary Secretory Immunoglobulin A ◽  
Secretory Immunoglobulin

Purpose:To monitor resting salivary secretory immunoglobulin A (SIgA) levels in international soccer players during the short-term training period that precedes international match play.Methods:In a repeated-measure design, saliva samples were obtained from 13 outfield soccer players who participated in the training camps preceding 7 games (5 home and 2 away) of the 2014 FIFA World Cup qualifying campaign. Samples were obtained daily for 4 d preceding each game (and analyzed for SIgA using the IPRO oral-fluid-collection system) at match day minus 1 (MD-1), minus 2 (MD-2), minus 3 (MD-3), and minus 4 (MD-4).Results:SIgA displayed a progressive decline (P = .01) during the 4-d training period (MD-4, 365 ± 127 μg/mL; MD-3, 348 ± 154 μg/mL; MD-2, 290 ± 138 μg/mL; MD-1, 256 ± 90 μg/mL) such that MD-1 values were significantly lower (P = .01) than both MD-4 and MD-3. The 95% confidence intervals for the differences between MD-1 and MD-4 were –191 to –26 and between MD-1 and MD-3 were –155 to –28.Conclusions:Data demonstrate that a short-term soccer-training camp in preparation for international competition induces detectable perturbations to mucosal immunity. Future studies should monitor SIgA (as a practical and noninvasive measure of immunity) alongside internal and external measures of training load in an attempt to strategically individualize training and nutritional strategies that may support optimal preparation for high-level competition.

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