Induction and Distribution of Intestinal Immune Responses after Administration of Recombinant Cholera Toxin B Subunit in the Ileal Pouches of Colectomized Patients

2001 ◽  
Vol 69 (10) ◽  
pp. 6564-6564
Author(s):  
Jan Kilhamn ◽  
Hans Brevinge ◽  
Marianne Quiding-Järbrink ◽  
Ann-Mari Svennerholm ◽  
Marianne Jertborn
Keyword(s):  
Immune Responses ◽  
Cholera Toxin ◽  
Cholera Toxin B Subunit ◽  
Toxin B ◽  
Cholera Toxin B ◽  
B Subunit

Use of a genetic cholera toxin B subunit/allergen fusion molecule as mucosal delivery system with immunosuppressive activity against Th2 immune responses

Vaccine ◽  
2007 ◽  
Vol 25 (50) ◽  
pp. 8395-8404 ◽  
Author(s):  
Merima Bublin ◽  
Elisabeth Hoflehner ◽  
Birgit Wagner ◽  
Christian Radauer ◽  
Stefan Wagner ◽  
...  
Keyword(s):  
Immune Responses ◽  
Cholera Toxin ◽  
Delivery System ◽  
Cholera Toxin B Subunit ◽  
Immunosuppressive Activity ◽  
Toxin B ◽  
Cholera Toxin B ◽  
B Subunit ◽  
Mucosal Delivery

scholarly journals Local and Systemic Immune Responses to Rectal Administration of Recombinant Cholera Toxin B Subunit in Humans

2001 ◽  
Vol 69 (6) ◽  
pp. 4125-4128 ◽  
Author(s):  
Marianne Jertborn ◽  
Inger Nordström ◽  
Anders Kilander ◽  
Cecil Czerkinsky ◽  
Jan Holmgren
Keyword(s):  
Immune Responses ◽  
Cholera Toxin ◽  
Immunoglobulin A ◽  
Rectal Administration ◽  
Antibody Responses ◽  
Cholera Toxin B Subunit ◽  
Toxin B ◽  
Cholera Toxin B ◽  
B Subunit ◽  
Antibody Secreting Cells

ABSTRACT The induction of immune responses to rectally administered recombinant cholera toxin B subunit (CTB) in humans was studied. Three immunizations induced high levels of CTB-specific antibody-secreting cells, particular of the immunoglobulin A isotype, in both rectum and peripheral blood. Antitoxin antibody responses in rectal secretions and serum were also found.

Enhancement of enteric mucosal immune responses by cholera toxin or cholera toxin B-subunit

1990 ◽  
pp. 371-372
Author(s):  
P J van der Heijden ◽  
A T J Bianchi ◽  
M A Dol ◽  
J W Pals ◽  
W Stok ◽  
...  
Keyword(s):  
Immune Responses ◽  
Cholera Toxin ◽  
Cholera Toxin B Subunit ◽  
Toxin B ◽  
Cholera Toxin B ◽  
B Subunit ◽  
Mucosal Immune Responses

scholarly journals Transcutaneous Immunization with Cholera Toxin B Subunit Adjuvant Suppresses IgE Antibody Responses Via Selective Induction of Th1 Immune Responses

2003 ◽  
Vol 170 (3) ◽  
pp. 1586-1592 ◽  
Author(s):  
Fabienne Anjuère ◽  
Annie George-Chandy ◽  
Florence Audant ◽  
Déborah Rousseau ◽  
Jan Holmgren ◽  
...  
Keyword(s):  
Immune Responses ◽  
Cholera Toxin ◽  
Antibody Responses ◽  
Cholera Toxin B Subunit ◽  
Toxin B ◽  
Cholera Toxin B ◽  
Ige Antibody ◽  
B Subunit ◽  
Transcutaneous Immunization ◽  
Th1 Immune Responses

scholarly journals Induction and Distribution of Intestinal Immune Responses after Administration of Recombinant Cholera Toxin B Subunit in the Ileal Pouches of Colectomized Patients

2001 ◽  
Vol 69 (5) ◽  
pp. 3466-3471 ◽  
Author(s):  
Jan Kilhamn ◽  
Hans Brevinge ◽  
Marianne Quiding-Järbrink ◽  
Ann-Mari Svennerholm ◽  
Marianne Jertborn
Keyword(s):  
Immune Responses ◽  
Cholera Toxin ◽  
Peripheral Blood ◽  
Ileal Pouch ◽  
Immunoglobulin A ◽  
Cholera Toxin B Subunit ◽  
Toxin B ◽  
Antibody Secreting Cell ◽  
Cholera Toxin B ◽  
B Subunit

ABSTRACT The induction and dissemination of mucosal immune responses to recombinant cholera toxin B subunit (rCTB) administered into the ileal pouches of patients, who had been colectomized because of ulcerative colitis, was analyzed. Biopsies from the duodenum and ileal pouch were collected, along with peripheral blood and ileostomy fluids. Two immunizations induced strong CTB-specific immunoglobulin A (IgA) antibody-secreting cell (ASC) responses in the duodenum in five of five patients, whereas weaker and less-frequent ASC responses were noted in the ileal pouch. Intestine-derived CTB-specific IgA ASCs were found in peripheral blood in three of the five patients. The vaccination also induced significant IgA antitoxin titer rises in ileostomy fluid in all of the patients. Increased production of gamma interferon in cell cultures from the ileal pouch was found in four of five patients after the vaccination. These results clearly indicate that rCTB administered into the distal ileum is capable of inducing B-cell responses in the “entire” small intestine and that homing of immunocompetent cells occurs preferentially to the duodenum.

scholarly journals Kinetics of Local and Systemic Immune Responses after Vaginal Immunization with Recombinant Cholera Toxin B Subunit in Humans

2005 ◽  
Vol 12 (3) ◽  
pp. 447-452 ◽  
Author(s):  
Lotta Wassen ◽  
Marianne Jertborn
Keyword(s):  
Immune Responses ◽  
Cholera Toxin ◽  
Female Genital Tract ◽  
Antibody Responses ◽  
Cholera Toxin B Subunit ◽  
Igg Antibody ◽  
Toxin B ◽  
Cholera Toxin B ◽  
B Subunit ◽  
Kinetics Of

ABSTRACT Vaginal vaccination seems to be the best strategy for inducing specific immunoglobulin A (IgA) and IgG antibody responses in the female genital tract. The relative efficiencies of one, two, and three vaginal doses of recombinant cholera toxin B subunit (CTB) in generating mucosal and systemic immune responses in healthy women were evaluated, and the kinetics of the immune responses were monitored for responding volunteers for up to 12 months after the last vaccination. A single dose of CTB failed to generate CTB-specific IgA antibody responses in cervical secretions. Two vaccinations induced significant increases in IgA antitoxin titers in seven of nine volunteers, and four volunteers also developed IgG antitoxin responses. The magnitudes of the responses were 20-fold for IgA antitoxin and 7.1-fold for IgG antitoxin. A third vaccination did not significantly increase the antitoxin responses, although the frequency of IgG responses was slightly higher than that after the second vaccination. In serum, CTB-specific antibodies were observed already after a single vaccination. However, two vaccinations were required to induce marked IgA as well as IgG antitoxin titer increases in the majority of volunteers. The postvaccination levels of antitoxin antibodies in serum were comparable after two and three vaccinations. At 12 months after vaccination, significantly elevated IgA and IgG antitoxin levels in cervical secretions could still be detected in approximately half of the volunteers who had initially responded to the vaccine. Antitoxin titer increases in serum were found in most of the vaccinees at follow-up.

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