Induction of mucosal and systemic immune responses by intranasal immunization using recombinant cholera toxin B subunit as an adjuvant

ABSTRACT The induction of immune responses to rectally administered recombinant cholera toxin B subunit (CTB) in humans was studied. Three immunizations induced high levels of CTB-specific antibody-secreting cells, particular of the immunoglobulin A isotype, in both rectum and peripheral blood. Antitoxin antibody responses in rectal secretions and serum were also found.

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Intranasal immunization of mice with recombinant protein antigen of serotype cStreptococcus mutans and cholera toxin B subunit

Archives of Oral Biology ◽

10.1016/0003-9969(90)90211-r ◽

1990 ◽

Vol 35 (6) ◽

pp. 475-477 ◽

Cited By ~ 14

Author(s):

I. Takahashi ◽

N. Okahashi ◽

T. Kanamoto ◽

H. Asakawa ◽

T. Koga

Keyword(s):

Recombinant Protein ◽

Cholera Toxin ◽

Protein Antigen ◽

Cholera Toxin B Subunit ◽

Toxin B ◽

Intranasal Immunization ◽

Cholera Toxin B ◽

B Subunit

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Intranasal Immunization of Mice with Group B Streptococcal Protein Rib and Cholera Toxin B Subunit Confers Protection against Lethal Infection

Infection and Immunity ◽

10.1128/iai.72.2.1184-1187.2004 ◽

2004 ◽

Vol 72 (2) ◽

pp. 1184-1187 ◽

Cited By ~ 21

Author(s):

Charlotte Larsson ◽

Jan Holmgren ◽

Gunnar Lindahl ◽

Charlotta Bergquist

Keyword(s):

Cholera Toxin ◽

Group B Streptococcus ◽

Surface Protein ◽

Cholera Toxin B Subunit ◽

Toxin B ◽

Intranasal Immunization ◽

Cholera Toxin B ◽

B Subunit ◽

A Cell ◽

Group B

ABSTRACT Intranasal immunization of mice with Rib, a cell surface protein of group B streptococcus (GBS), conjugated to or simply coadministered with the recombinant cholera toxin B subunit, induces systemic immunoglobulin G (IgG) and local IgA antibody responses and confers protection against lethal GBS infection. These findings have implications for the development of a human GBS vaccine.

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Intranasal Immunization with the Cholera Toxin B Subunit-Pneumococcal Surface Antigen A Fusion Protein Induces Protection against Colonization with Streptococcus pneumoniae and Has Negligible Impact on the Nasopharyngeal and Oral Microbiota of Mice

Infection and Immunity ◽

10.1128/iai.00134-06 ◽

2006 ◽

Vol 74 (8) ◽

pp. 4939-4944 ◽

Cited By ~ 30

Author(s):

F. C. Pimenta ◽

E. N. Miyaji ◽

A. P. M. Arêas ◽

M. L. S. Oliveira ◽

A. L. S. S. de Andrade ◽

...

Keyword(s):

Streptococcus Pneumoniae ◽

Fusion Protein ◽

Cholera Toxin ◽

Surface Antigen ◽

Cholera Toxin B Subunit ◽

Toxin B ◽

Intranasal Immunization ◽

Cholera Toxin B ◽

B Subunit ◽

Antigen A

ABSTRACT One of the candidate proteins for a mucosal vaccine antigen against Streptococcus pneumoniae is PsaA (pneumococcal surface antigen A). Vaccines targeting mucosal immunity may raise concerns as to possible alterations in the normal microbiota, especially in the case of PsaA, which was shown to have homologs with elevated sequence identity in other viridans group streptococci. In this work, we demonstrate that intranasal immunization with a cholera toxin B subunit-PsaA fusion protein is able to protect mice against colonization with S. pneumoniae but does not significantly alter the natural oral or nasopharyngeal microbiota of mice.

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