scholarly journals Replication-Dependent Recruitment of the β-Subunit of DNA Polymerase III from Cytosolic Spaces to Replication Forks in Escherichia coli

2002 ◽  
Vol 184 (3) ◽  
pp. 867-870 ◽  
Author(s):  
Toshinari Onogi ◽  
Katsufumi Ohsumi ◽  
Tsutomu Katayama ◽  
Sota Hiraga
Keyword(s):  
Escherichia Coli ◽  
Dna Polymerase ◽  
Chromosome Replication ◽  
Temperature Sensitive ◽  
Replication Forks ◽  
Dna Polymerase Iii ◽  
Β Subunit ◽  
Nonpermissive Temperature ◽  
Polymerase Iii

ABSTRACT The β-subunit of DNA polymerase III is located as one or two condensed clusters within the nucleoid-occupied space in exponentially growing cells of Escherichia coli. When chromosome replication is terminated after incubation at nonpermissive temperature in a temperature-sensitive dnaC mutant, the β-subunit is located in the cytosolic spaces of the cell poles.

scholarly journals INTERACTION OF DNA POLYMERASE III γ AND β SUBUNITS IN VIVO IN SALMONELLA TYPHIMURIUM

Genetics ◽  
1986 ◽  
Vol 113 (3) ◽  
pp. 499-515
Author(s):  
Joyce Engstrom ◽  
Annette Wong ◽  
Russell Maurer
Keyword(s):  
Salmonella Typhimurium ◽  
Dna Polymerase ◽  
Regulatory Gene ◽  
Temperature Sensitive ◽  
Physical Interaction ◽  
Dna Polymerase Iii ◽  
Β Subunit ◽  
Γ Subunit ◽  
Polymerase Iii

ABSTRACT We show that temperature-sensitive mutations in dnaZ, the gene for the γ subunit of DNA polymerase III holoenzyme, can be suppressed by mutations in the dnaN gene, which encodes the β subunit. These results support a direct physical interaction of these two subunits during polymerase assembly or function. The suppressor phenotype is also sensitive to modulation by the dnaA genotype. Since dnaA is organized in an operon with dnaN, and dnaA is a regulatory gene of this operon, we propose that the dnaA effect on suppression can best be explained by modulation of suppressor dnaN levels.

scholarly journals β*, a UV-inducible Smaller Form of the β Subunit Sliding Clamp of DNA Polymerase III of Escherichia coli

1996 ◽  
Vol 271 (5) ◽  
pp. 2482-2490 ◽  
Author(s):  
Tamar Paz-Elizur ◽  
Rami Skaliter ◽  
Sara Blumenstein ◽  
Zvi Livneh
Keyword(s):  
Escherichia Coli ◽  
Dna Polymerase ◽  
Dna Polymerase Iii ◽  
Β Subunit ◽  
Sliding Clamp ◽  
Polymerase Iii

scholarly journals The interaction of DNA polymerase III and the product of the Escherichia coli mutator gene, mutD.

1984 ◽  
Vol 259 (9) ◽  
pp. 5567-5573
Author(s):  
R DiFrancesco ◽  
S K Bhatnagar ◽  
A Brown ◽  
M J Bessman
Keyword(s):  
Escherichia Coli ◽  
Dna Polymerase ◽  
Dna Polymerase Iii ◽  
Mutator Gene ◽  
Polymerase Iii
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