scholarly journals Genetic Classification of Severe Early Childhood Caries by Use of Subtracted DNA Fragments from Streptococcus mutans

2008 ◽  
Vol 46 (9) ◽  
pp. 2868-2873 ◽  
Author(s):  
D. Saxena ◽  
P. W. Caufield ◽  
Y. Li ◽  
S. Brown ◽  
J. Song ◽  
...  
Keyword(s):  
Early Childhood ◽  
Streptococcus Mutans ◽  
Early Childhood Caries ◽  
Dna Fragments ◽  
Genetic Classification ◽  
Severe Early Childhood Caries ◽  
Childhood Caries

Streptococcus mutans isolated from children with severe-early childhood caries form higher levels of persisters

2020 ◽  
Vol 110 ◽  
pp. 104601 ◽  
Author(s):  
Aaron Bottner ◽  
Richard Y. He ◽  
Andrea Sarbu ◽  
S.M. Hashim Nainar ◽  
Delphine Dufour ◽  
...  
Keyword(s):  
Early Childhood ◽  
Streptococcus Mutans ◽  
Early Childhood Caries ◽  
Severe Early Childhood Caries ◽  
Childhood Caries

scholarly journals The enhancing antibiofilm activity of curcumin on Streptococcus mutans strains from severe early childhood caries

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Bingchun Li ◽  
Ting Pan ◽  
Huancai Lin ◽  
Yan Zhou
Keyword(s):  
Early Childhood ◽  
Streptococcus Mutans ◽  
Early Childhood Caries ◽  
Antibiofilm Activity ◽  
Curcumin Treatment ◽  
Clinical Strains ◽  
Live Bacteria ◽  
Total Bacteria ◽  
Severe Early Childhood Caries ◽  
Childhood Caries

Abstract Background Streptococcus mutans (S. mutans) is one of the main cariogenic bacteria for caries. It was found that the clinical strains of S. mutans isolated from caries active population have stronger cariogenic ability than the isolates from caries-free (CF) people. Previous studies have found that curcumin can inhibit biofilm formation of S. mutans UA159. The objective of this study is to explore the antibiofilm effect of curcumin on the clinical isolates of S. mutans from severe early childhood caries(SECC). Results The isolates from SECC group had more biomass than CF group (t = 4.296, P < 0.001). The acidogenicity and aciduricity of the strains from two groups showed no significant difference. After treatment with curcumin, the viability of biofilm was reduced to 61.865% ± 7.108% in SECC and to 84.059% ± 10.227% in CF group at 24 h (P < 0.05). The net reduction of live bacteria and total bacteria in the SECC group was significantly higher than that of the CF group (live bacteria t = 3.305, P = 0.016; total bacteria t = 2.378, P = 0.045) at 5 min. For 24 h, the net reduction of live bacteria and total bacteria in the SECC group was significantly higher than that of the CF group (live bacteria t = 3.305, P = 0.016; total bacteria t = 2.378, P = 0.045). The reduction of biofilm thickness reduced significantly in 5 min (t = 4.110, P = 0.015) and in 24 h (t = 3.453, P = 0.014). Long-term (24 h) curcumin treatment inhibited the amount of EPS in SECC group from (25.980 ± 1.156) μm3/μm2 to (20.136 ± 1.042) μm3/μm2, the difference was statistically significant (t = 7.510, P < 0.001). The gene of gtfC, gtfD, ftf, gbpB, fruA and srtA in the CF group and the gtfB, gtfC, gtfD, ftf, gbpB, srtA in SECC group were respectively reduced after 5 min curcumin treatment. After 24 h treatment, the gtfB, gtfC, gtfD, ftf, gbpB, fruA and srtA in both two groups were downregulation, all the differences were statistically significant. Conclusions Curcumin has antibiofilm activity on clinical strains of S. mutans, especially for those isolated from SECC.

scholarly journals The enhancing antibiofilm activity of curcumin on Streptococcus mutans strains from severe early childhood caries

2020 ◽  
Author(s):  
Bingchun Li ◽  
Ting Pan ◽  
Huancai Lin ◽  
Yan Zhou
Keyword(s):  
Early Childhood ◽  
Streptococcus Mutans ◽  
Early Childhood Caries ◽  
Antibiofilm Activity ◽  
Curcumin Treatment ◽  
Clinical Strains ◽  
Live Bacteria ◽  
Total Bacteria ◽  
Severe Early Childhood Caries ◽  
Childhood Caries

Abstract Background Streptococcus mutans ( S. mutans ) is one of the main cariogenic bacteria for caries. It was found that the clinical strains of S. mutans isolated from caries active population have stronger cariogenic ability than the isolates from caries-free (CF) people. Previous studies have found that curcumin can inhibit biofilm formation of S. mutans UA159. The objective of this study is to explore the antibiofilm effect of curcumin on the clinical isolates of S. mutans from severe early childhood caries(SECC). Results The isolates from SECC group had more biomass than CF group (t=4.296, P<0.001). The acidogenicity and aciduricity of the strains from two groups showed no significant difference. After treatment with curcumin, the viability of biofilm was reduced to 61.865%±7.108% in SECC and to 84.059%±10.227% in CF group at 24 h (P<0.05). The net reduction of live bacteria and total bacteria in the SECC group was significantly higher than that of the CF group (live bacteria t=3.305, P=0.016; total bacteria t=2.378, P=0.045) at 5min. For 24h, the net reduction of live bacteria and total bacteria in the SECC group was significantly higher than that of the CF group (live bacteria t=3.305, P=0.016; total bacteria t=2.378, P=0.045). The reduction of biofilm thickness reduced significantly in 5min (t = 4.110, P = 0.015) and in 24h (t=3.453, P =0.014). Long-term (24h) curcumin treatment inhibited the amount of EPS in SECC group from (25.980±1.156) μm 3 /μm 2 to (20.136±1.042) μm 3 /μm 2 , the difference was statistically significant (t=7.510, P<0.001). The gene of gtfC, gtfD, ftf, gbpB, fruA and srtA in the CF group and the gtfB, gtfC, gtfD, ftf, gbpB, srtA in SECC group were respectively reduced after 5min curcumin treatment. After 24h treatment, the gtfB, gtfC, gtfD, ftf, gbpB, fruA and srtA in both two groups were downregulation, all the differences were statistically significant. Conclusions Curcumin has antibiofilm activity on clinical strains of S. mutans , especially for those isolated from SECC.

scholarly journals The enhancing antibiofilm activity of curcumin on Streptococcus mutans strains from severe early childhood caries

2020 ◽  
Author(s):  
Bingchun Li ◽  
Ting Pan ◽  
Huancai Lin ◽  
Yan Zhou
Keyword(s):  
Early Childhood ◽  
Streptococcus Mutans ◽  
Early Childhood Caries ◽  
Antibiofilm Activity ◽  
Curcumin Treatment ◽  
Clinical Strains ◽  
Live Bacteria ◽  
Total Bacteria ◽  
Severe Early Childhood Caries ◽  
Childhood Caries

Abstract Background Streptococcus mutans (S. mutans) is one of the main cariogenic bacteria for caries. It was found that the clinical strains of S. mutans isolated from caries active population have stronger cariogenic ability than the isolates from caries-free (CF) people. Previous studies have found that curcumin can inhibit biofilm formation of S. mutans UA159. The objective of this study is to explore the antibiofilm effect of curcumin on the clinical isolates of S. mutans from severe early childhood caries(SECC).Results The isolates from SECC group had more biomass than CF group (t = 4.296, P < 0.001). The acidogenicity and aciduricity of the strains from two groups showed no significant difference. After treatment with curcumin, the viability of biofilm was reduced to 61.865%±7.108% in SECC and to 84.059%±10.227% in CF group at 24 h (P < 0.05). The net reduction of live bacteria and total bacteria in the SECC group was significantly higher than that of the CF group (live bacteria t = 3.305, P = 0.016; total bacteria t = 2.378, P = 0.045) at 5min. For 24h, the net reduction of live bacteria and total bacteria in the SECC group was significantly higher than that of the CF group (live bacteria t = 3.305, P = 0.016; total bacteria t = 2.378, P = 0.045). The reduction of biofilm thickness reduced significantly in 5min (t = 4.110, P = 0.015) and in 24h (t = 3.453, P = 0.014). Long-term (24h) curcumin treatment inhibited the amount of EPS in SECC group from (25.980±1.156) μm3/μm2 to (20.136±1.042) μm3/μm2, the difference was statistically significant (t = 7.510, P < 0.001). The gene of gtfC, gtfD, ftf, gbpB, fruA and srtA in the CF group and the gtfB, gtfC, gtfD, ftf, gbpB, srtA in SECC group were respectively reduced after 5min curcumin treatment. After 24h treatment, the gtfB, gtfC, gtfD, ftf, gbpB, fruA and srtA in both two groups were downregulation, all the differences were statistically significant.Conclusions Curcumin has antibiofilm activity on clinical strains of S. mutans, especially for those isolated from SECC.

scholarly journals Characterization of Supragingival Plaque and Oral Swab Microbiomes in Children With Severe Early Childhood Caries

2021 ◽  
Vol 12 ◽  
Author(s):  
Vivianne Cruz de Jesus ◽  
Mohd Wasif Khan ◽  
Betty-Anne Mittermuller ◽  
Kangmin Duan ◽  
Pingzhao Hu ◽  
...  
Keyword(s):  
Early Childhood ◽  
Dental Plaque ◽  
Early Childhood Caries ◽  
Oral Microbiome ◽  
Rrna Genes ◽  
Oral Swab ◽  
Supragingival Plaque ◽  
Severe Early Childhood Caries ◽  
Childhood Caries

The human oral cavity harbors one of the most diverse microbial communities with different oral microenvironments allowing the colonization of unique microbial species. This study aimed to determine which of two commonly used sampling sites (dental plaque vs. oral swab) would provide a better prediction model for caries-free vs. severe early childhood caries (S-ECC) using next generation sequencing and machine learning (ML). In this cross-sectional study, a total of 80 children (40 S-ECC and 40 caries-free) &lt; 72 months of age were recruited. Supragingival plaque and oral swab samples were used for the amplicon sequencing of the V4-16S rRNA and ITS1 rRNA genes. The results showed significant differences in alpha and beta diversity between dental plaque and oral swab bacterial and fungal microbiomes. Differential abundance analyses showed that, among others, the cariogenic species Streptococcus mutans was enriched in the dental plaque, compared to oral swabs, of children with S-ECC. The fungal species Candida dubliniensis and C. tropicalis were more abundant in the oral swab samples of children with S-ECC compared to caries-free controls. They were also among the top 20 most important features for the classification of S-ECC vs. caries-free in oral swabs and for the classification of dental plaque vs. oral swab in the S-ECC group. ML approaches revealed the possibility of classifying samples according to both caries status and sampling sites. The tested site of sample collection did not change the predictability of the disease. However, the species considered to be important for the classification of disease in each sampling site were slightly different. Being able to determine the origin of the samples could be very useful during the design of oral microbiome studies. This study provides important insights into the differences between the dental plaque and oral swab bacteriome and mycobiome of children with S-ECC and those caries-free.

scholarly journals The enhancing antibiofilm activity of curcumin on Streptococcus mutans strains from severe early childhood caries

2020 ◽  
Author(s):  
Bingchun Li ◽  
Ting Pan ◽  
Huancai Lin ◽  
Yan Zhou
Keyword(s):  
Early Childhood ◽  
Streptococcus Mutans ◽  
Early Childhood Caries ◽  
Antibiofilm Activity ◽  
Curcumin Treatment ◽  
Clinical Strains ◽  
Live Bacteria ◽  
Total Bacteria ◽  
Severe Early Childhood Caries ◽  
Childhood Caries

Abstract BackgroundStreptococcus mutans (S. mutans) is one of the main cariogenic bacteria for caries. It was found that the clinical strains of S. mutans isolated from caries active population have stronger cariogenic ability than the isolates from caries-free (CF) people. Previous studies have found that curcumin can inhibit biofilm formation of S. mutans UA159. The objective of this study is to explore the antibiofilm effect of curcumin on the clinical isolates of S. mutans from severe early childhood caries(SECC).ResultsThe isolates from SECC group had more biomass than CF group (t = 4.296, P < 0.001). The acidogenicity and aciduricity of the strains from two groups showed no significant difference. After treatment with curcumin, the viability of biofilm was reduced to 61.865%±7.108% in SECC and to 84.059%±10.227% in CF group at 24 h (P < 0.05). The net reduction of live bacteria and total bacteria in the SECC group was significantly higher than that of the CF group (live bacteria t = 3.305, P = 0.016; total bacteria t = 2.378, P = 0.045) at 5 min. For 24 h, the net reduction of live bacteria and total bacteria in the SECC group was significantly higher than that of the CF group (live bacteria t = 3.305, P = 0.016; total bacteria t = 2.378, P = 0.045). The reduction of biofilm thickness reduced significantly in 5 min (t = 4.110, P = 0.015) and in 24 h (t = 3.453, P = 0.014). Long-term (24 h) curcumin treatment inhibited the amount of EPS in SECC group from (25.980 ± 1.156) µm3/µm2 to (20.136 ± 1.042) µm3/µm2, the difference was statistically significant (t = 7.510, P < 0.001). The gene of gtfC, gtfD, ftf, gbpB, fruA and srtA in the CF group and the gtfB, gtfC, gtfD, ftf, gbpB, srtA in SECC group were respectively reduced after 5 min curcumin treatment. After 24 h treatment, the gtfB, gtfC, gtfD, ftf, gbpB, fruA and srtA in both two groups were downregulation, all the differences were statistically significant.ConclusionsCurcumin has antibiofilm activity on clinical strains of S. mutans, especially for those isolated from SECC.

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