scholarly journals Genetic polymorphism of scrA gene of Streptococcus mutans isolates is not associated with biofilm formation in severe early childhood caries

2017 ◽  
Vol 17 (1) ◽  
Author(s):  
Yan Zhou ◽  
Lixia Yu ◽  
Ye Tao ◽  
Qinghui Zhi ◽  
Huancai Lin
Keyword(s):  
Early Childhood ◽  
Genetic Polymorphism ◽  
Streptococcus Mutans ◽  
Biofilm Formation ◽  
Early Childhood Caries ◽  
Severe Early Childhood Caries ◽  
Childhood Caries

Streptococcus mutans isolated from children with severe-early childhood caries form higher levels of persisters

2020 ◽  
Vol 110 ◽  
pp. 104601 ◽  
Author(s):  
Aaron Bottner ◽  
Richard Y. He ◽  
Andrea Sarbu ◽  
S.M. Hashim Nainar ◽  
Delphine Dufour ◽  
...  
Keyword(s):  
Early Childhood ◽  
Streptococcus Mutans ◽  
Early Childhood Caries ◽  
Severe Early Childhood Caries ◽  
Childhood Caries

scholarly journals Bacterial diversity and functional analysis of severe early childhood caries and recurrence in India

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Balakrishnan Kalpana ◽  
Puniethaa Prabhu ◽  
Ashaq Hussain Bhat ◽  
Arunsaikiran Senthilkumar ◽  
Raj Pranap Arun ◽  
...  
Keyword(s):  
Early Childhood ◽  
Bacterial Diversity ◽  
Biofilm Formation ◽  
Early Childhood Caries ◽  
Oral Microbiome ◽  
Oral Disease ◽  
Indian Children ◽  
Severe Early Childhood Caries ◽  
Childhood Caries

AbstractDental caries is the most prevalent oral disease affecting nearly 70% of children in India and elsewhere. Micro-ecological niche based acidification due to dysbiosis in oral microbiome are crucial for caries onset and progression. Here we report the tooth bacteriome diversity compared in Indian children with caries free (CF), severe early childhood caries (SC) and recurrent caries (RC). High quality V3–V4 amplicon sequencing revealed that SC exhibited high bacterial diversity with unique combination and interrelationship. Gracillibacteria_GN02 and TM7 were unique in CF and SC respectively, while Bacteroidetes, Fusobacteria were significantly high in RC. Interestingly, we found Streptococcus oralis subsp. tigurinus clade 071 in all groups with significant abundance in SC and RC. Positive correlation between low and high abundant bacteria as well as with TCS, PTS and ABC transporters were seen from co-occurrence network analysis. This could lead to persistence of SC niche resulting in RC. Comparative in vitro assessment of biofilm formation showed that the standard culture of S. oralis and its phylogenetically similar clinical isolates showed profound biofilm formation and augmented the growth and enhanced biofilm formation in S. mutans in both dual and multispecies cultures.

scholarly journals The enhancing antibiofilm activity of curcumin on Streptococcus mutans strains from severe early childhood caries

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Bingchun Li ◽  
Ting Pan ◽  
Huancai Lin ◽  
Yan Zhou
Keyword(s):  
Early Childhood ◽  
Streptococcus Mutans ◽  
Early Childhood Caries ◽  
Antibiofilm Activity ◽  
Curcumin Treatment ◽  
Clinical Strains ◽  
Live Bacteria ◽  
Total Bacteria ◽  
Severe Early Childhood Caries ◽  
Childhood Caries

Abstract Background Streptococcus mutans (S. mutans) is one of the main cariogenic bacteria for caries. It was found that the clinical strains of S. mutans isolated from caries active population have stronger cariogenic ability than the isolates from caries-free (CF) people. Previous studies have found that curcumin can inhibit biofilm formation of S. mutans UA159. The objective of this study is to explore the antibiofilm effect of curcumin on the clinical isolates of S. mutans from severe early childhood caries(SECC). Results The isolates from SECC group had more biomass than CF group (t = 4.296, P < 0.001). The acidogenicity and aciduricity of the strains from two groups showed no significant difference. After treatment with curcumin, the viability of biofilm was reduced to 61.865% ± 7.108% in SECC and to 84.059% ± 10.227% in CF group at 24 h (P < 0.05). The net reduction of live bacteria and total bacteria in the SECC group was significantly higher than that of the CF group (live bacteria t = 3.305, P = 0.016; total bacteria t = 2.378, P = 0.045) at 5 min. For 24 h, the net reduction of live bacteria and total bacteria in the SECC group was significantly higher than that of the CF group (live bacteria t = 3.305, P = 0.016; total bacteria t = 2.378, P = 0.045). The reduction of biofilm thickness reduced significantly in 5 min (t = 4.110, P = 0.015) and in 24 h (t = 3.453, P = 0.014). Long-term (24 h) curcumin treatment inhibited the amount of EPS in SECC group from (25.980 ± 1.156) μm3/μm2 to (20.136 ± 1.042) μm3/μm2, the difference was statistically significant (t = 7.510, P < 0.001). The gene of gtfC, gtfD, ftf, gbpB, fruA and srtA in the CF group and the gtfB, gtfC, gtfD, ftf, gbpB, srtA in SECC group were respectively reduced after 5 min curcumin treatment. After 24 h treatment, the gtfB, gtfC, gtfD, ftf, gbpB, fruA and srtA in both two groups were downregulation, all the differences were statistically significant. Conclusions Curcumin has antibiofilm activity on clinical strains of S. mutans, especially for those isolated from SECC.

scholarly journals The enhancing antibiofilm activity of curcumin on Streptococcus mutans strains from severe early childhood caries

2020 ◽  
Author(s):  
Bingchun Li ◽  
Ting Pan ◽  
Huancai Lin ◽  
Yan Zhou
Keyword(s):  
Early Childhood ◽  
Streptococcus Mutans ◽  
Early Childhood Caries ◽  
Antibiofilm Activity ◽  
Curcumin Treatment ◽  
Clinical Strains ◽  
Live Bacteria ◽  
Total Bacteria ◽  
Severe Early Childhood Caries ◽  
Childhood Caries

Abstract Background Streptococcus mutans ( S. mutans ) is one of the main cariogenic bacteria for caries. It was found that the clinical strains of S. mutans isolated from caries active population have stronger cariogenic ability than the isolates from caries-free (CF) people. Previous studies have found that curcumin can inhibit biofilm formation of S. mutans UA159. The objective of this study is to explore the antibiofilm effect of curcumin on the clinical isolates of S. mutans from severe early childhood caries(SECC). Results The isolates from SECC group had more biomass than CF group (t=4.296, P<0.001). The acidogenicity and aciduricity of the strains from two groups showed no significant difference. After treatment with curcumin, the viability of biofilm was reduced to 61.865%±7.108% in SECC and to 84.059%±10.227% in CF group at 24 h (P<0.05). The net reduction of live bacteria and total bacteria in the SECC group was significantly higher than that of the CF group (live bacteria t=3.305, P=0.016; total bacteria t=2.378, P=0.045) at 5min. For 24h, the net reduction of live bacteria and total bacteria in the SECC group was significantly higher than that of the CF group (live bacteria t=3.305, P=0.016; total bacteria t=2.378, P=0.045). The reduction of biofilm thickness reduced significantly in 5min (t = 4.110, P = 0.015) and in 24h (t=3.453, P =0.014). Long-term (24h) curcumin treatment inhibited the amount of EPS in SECC group from (25.980±1.156) μm 3 /μm 2 to (20.136±1.042) μm 3 /μm 2 , the difference was statistically significant (t=7.510, P<0.001). The gene of gtfC, gtfD, ftf, gbpB, fruA and srtA in the CF group and the gtfB, gtfC, gtfD, ftf, gbpB, srtA in SECC group were respectively reduced after 5min curcumin treatment. After 24h treatment, the gtfB, gtfC, gtfD, ftf, gbpB, fruA and srtA in both two groups were downregulation, all the differences were statistically significant. Conclusions Curcumin has antibiofilm activity on clinical strains of S. mutans , especially for those isolated from SECC.

scholarly journals The enhancing antibiofilm activity of curcumin on Streptococcus mutans strains from severe early childhood caries

2020 ◽  
Author(s):  
Bingchun Li ◽  
Ting Pan ◽  
Huancai Lin ◽  
Yan Zhou
Keyword(s):  
Early Childhood ◽  
Streptococcus Mutans ◽  
Early Childhood Caries ◽  
Antibiofilm Activity ◽  
Curcumin Treatment ◽  
Clinical Strains ◽  
Live Bacteria ◽  
Total Bacteria ◽  
Severe Early Childhood Caries ◽  
Childhood Caries

Abstract Background Streptococcus mutans (S. mutans) is one of the main cariogenic bacteria for caries. It was found that the clinical strains of S. mutans isolated from caries active population have stronger cariogenic ability than the isolates from caries-free (CF) people. Previous studies have found that curcumin can inhibit biofilm formation of S. mutans UA159. The objective of this study is to explore the antibiofilm effect of curcumin on the clinical isolates of S. mutans from severe early childhood caries(SECC).Results The isolates from SECC group had more biomass than CF group (t = 4.296, P < 0.001). The acidogenicity and aciduricity of the strains from two groups showed no significant difference. After treatment with curcumin, the viability of biofilm was reduced to 61.865%±7.108% in SECC and to 84.059%±10.227% in CF group at 24 h (P < 0.05). The net reduction of live bacteria and total bacteria in the SECC group was significantly higher than that of the CF group (live bacteria t = 3.305, P = 0.016; total bacteria t = 2.378, P = 0.045) at 5min. For 24h, the net reduction of live bacteria and total bacteria in the SECC group was significantly higher than that of the CF group (live bacteria t = 3.305, P = 0.016; total bacteria t = 2.378, P = 0.045). The reduction of biofilm thickness reduced significantly in 5min (t = 4.110, P = 0.015) and in 24h (t = 3.453, P = 0.014). Long-term (24h) curcumin treatment inhibited the amount of EPS in SECC group from (25.980±1.156) μm3/μm2 to (20.136±1.042) μm3/μm2, the difference was statistically significant (t = 7.510, P < 0.001). The gene of gtfC, gtfD, ftf, gbpB, fruA and srtA in the CF group and the gtfB, gtfC, gtfD, ftf, gbpB, srtA in SECC group were respectively reduced after 5min curcumin treatment. After 24h treatment, the gtfB, gtfC, gtfD, ftf, gbpB, fruA and srtA in both two groups were downregulation, all the differences were statistically significant.Conclusions Curcumin has antibiofilm activity on clinical strains of S. mutans, especially for those isolated from SECC.

scholarly journals The enhancing antibiofilm activity of curcumin on Streptococcus mutans strains from severe early childhood caries

2020 ◽  
Author(s):  
Bingchun Li ◽  
Ting Pan ◽  
Huancai Lin ◽  
Yan Zhou
Keyword(s):  
Early Childhood ◽  
Streptococcus Mutans ◽  
Early Childhood Caries ◽  
Antibiofilm Activity ◽  
Curcumin Treatment ◽  
Clinical Strains ◽  
Live Bacteria ◽  
Total Bacteria ◽  
Severe Early Childhood Caries ◽  
Childhood Caries

Abstract BackgroundStreptococcus mutans (S. mutans) is one of the main cariogenic bacteria for caries. It was found that the clinical strains of S. mutans isolated from caries active population have stronger cariogenic ability than the isolates from caries-free (CF) people. Previous studies have found that curcumin can inhibit biofilm formation of S. mutans UA159. The objective of this study is to explore the antibiofilm effect of curcumin on the clinical isolates of S. mutans from severe early childhood caries(SECC).ResultsThe isolates from SECC group had more biomass than CF group (t = 4.296, P < 0.001). The acidogenicity and aciduricity of the strains from two groups showed no significant difference. After treatment with curcumin, the viability of biofilm was reduced to 61.865%±7.108% in SECC and to 84.059%±10.227% in CF group at 24 h (P < 0.05). The net reduction of live bacteria and total bacteria in the SECC group was significantly higher than that of the CF group (live bacteria t = 3.305, P = 0.016; total bacteria t = 2.378, P = 0.045) at 5 min. For 24 h, the net reduction of live bacteria and total bacteria in the SECC group was significantly higher than that of the CF group (live bacteria t = 3.305, P = 0.016; total bacteria t = 2.378, P = 0.045). The reduction of biofilm thickness reduced significantly in 5 min (t = 4.110, P = 0.015) and in 24 h (t = 3.453, P = 0.014). Long-term (24 h) curcumin treatment inhibited the amount of EPS in SECC group from (25.980 ± 1.156) µm3/µm2 to (20.136 ± 1.042) µm3/µm2, the difference was statistically significant (t = 7.510, P < 0.001). The gene of gtfC, gtfD, ftf, gbpB, fruA and srtA in the CF group and the gtfB, gtfC, gtfD, ftf, gbpB, srtA in SECC group were respectively reduced after 5 min curcumin treatment. After 24 h treatment, the gtfB, gtfC, gtfD, ftf, gbpB, fruA and srtA in both two groups were downregulation, all the differences were statistically significant.ConclusionsCurcumin has antibiofilm activity on clinical strains of S. mutans, especially for those isolated from SECC.

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