scholarly journals Zika Virus Testing Considerations: Lessons Learned from the First 80 Real-Time Reverse Transcription-PCR-Positive Cases Diagnosed in New York State

2016 ◽  
Vol 55 (2) ◽  
pp. 535-544 ◽  
Author(s):  
Kirsten St. George ◽  
Inderbir S. Sohi ◽  
Elizabeth M. Dufort ◽  
Amy B. Dean ◽  
Jennifer L. White ◽  
...  
Keyword(s):  
New York ◽  
Pregnant Women ◽  
Real Time ◽  
Molecular Diagnosis ◽  
Reverse Transcription ◽  
Zika Virus ◽  
New York State ◽  
Lessons Learned ◽  
York State ◽  
Reverse Transcription Pcr

ABSTRACTThe performance and interpretation of laboratory tests for Zika virus (ZKV) continue to be evaluated. Serology is cross-reactive, laborious, and frequently difficult to interpret, and serum was initially solely recommended for molecular diagnosis. ZKV testing was initiated in January 2016 in New York State for symptomatic patients, pregnant women, their infants, and patients with Guillain-Barré syndrome who had traveled to areas with ZKV transmission. Subsequently, eligibility was expanded to pregnant women with sexual partners with similar travel histories. Serum and urine collected within 4 weeks of symptom onset or within 6 weeks of travel were tested with real-time reverse transcription-PCR (RT-PCR) assays targeting the ZKV envelope and NS2B genes. In this review of lessons learned from the first 80 positive cases in NYS, ZKV RNA was detected in urine only in 50 patients, in serum only in 19 patients, and in both samples concurrently in 11 patients, with average viral loads in urine a log higher than those in serum. Among 93 positive samples from the 80 patients, 41 were positive on both gene assays, 52 were positive on the envelope only, and none were positive on the NS2B only. Of the 80 infected patients, test results for 74 (93%) would have defined their infection status as not detected or equivocal if the requirement for positive results from two assay targets (two-target-positive requirement) in the initial federal guidance to public health laboratories was enforced, if urine was not tested, or if the extended eligibility time for molecular testing was not implemented. These changes facilitated more extensive molecular diagnosis of ZKV, reducing reliance on time-consuming and potentially inconclusive serology.

scholarly journals Evaluation of a Real-Time Reverse Transcription-PCR Assay for Detection of Enterovirus D68 in Clinical Samples from an Outbreak in New York State in 2014

2015 ◽  
Vol 53 (6) ◽  
pp. 1915-1920 ◽  
Author(s):  
Jian Zhuge ◽  
Eric Vail ◽  
Jeffrey L. Bush ◽  
Lauren Singelakis ◽  
Weihua Huang ◽  
...  
Keyword(s):  
New York ◽  
Real Time ◽  
Reverse Transcription ◽  
New York State ◽  
Respiratory Illness ◽  
Clinical Samples ◽  
Sequencing Analysis ◽  
York State ◽  
Reverse Transcription Pcr ◽  
Enterovirus D68

An outbreak of severe respiratory illness associated with enterovirus D68 (EV-D68) infection was reported in mid-August 2014 in the United States. In this study, we evaluated the diagnostic utility of an EV-D68-specific real-time reverse transcription-PCR (rRT-PCR) that was recently developed by the Centers for Disease Control and Prevention in clinical samples. Nasopharyngeal (NP) swab specimens from patients in a recent outbreak of respiratory illness in the lower Hudson Valley, New York State, were collected and examined for the presence of human rhinovirus or enterovirus using the FilmArray Respiratory Panel (RP) assay. Samples positive by RP were assessed using EV-D68 rRT-PCR, and the data were compared to results from sequencing analysis of partial VP1 and 5′ untranslated region (5′-UTR) sequences of the EV genome. A total of 285 RP-positive NP specimens (260 from the 2014 outbreak and 25 from 2013) were analyzed by rRT-PCR; EV-D68 was detected in 74 of 285 (26.0%) specimens examined. Data for comparisons between rRT-PCR and sequencing analysis were obtained from 194 NP specimens. EV-D68 detection was confirmed by sequencing analysis in 71 of 74 positive and in 1 of 120 randomly selected negative specimens by rRT-PCR. The EV-D68 rRT-PCR showed diagnostic sensitivity and specificity of 98.6% and 97.5%, respectively. Our data suggest that the EV-D68 rRT-PCR is a reliable assay for detection of EV-D68 in clinical samples and has a potential to be used as a tool for rapid diagnosis and outbreak investigation of EV-D68-associated infections in clinical and public health laboratories.

scholarly journals Proximity to risk-appropriate perinatal hospitals for pregnant women with congenital heart defects in New York state

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Lauren E. Schlichting ◽  
Tabassum Insaf ◽  
George Lui ◽  
Ali Zaidi ◽  
Alissa Van Zutphen
Keyword(s):  
New York ◽  
Pregnant Women ◽  
Congenital Heart Defects ◽  
Congenital Heart ◽  
Heart Defects ◽  
New York State ◽  
York State

Lessons Learned While Implementing Mandatory Health care–Associated Infection Reporting in New York State

2013 ◽  
Vol 19 (4) ◽  
pp. 294-299 ◽  
Author(s):  
Rachel L. Stricof ◽  
Carole Van Antwerpen ◽  
Perry F. Smith ◽  
Guthrie S. Birkhead
Keyword(s):  
Health Care ◽  
New York ◽  
New York State ◽  
Lessons Learned ◽  
York State ◽  
Health Care Associated Infection

Experiences of HIV/STD Providers in New York State During Hurricane Sandy: Strengths, Challenges, and Recommendations

2020 ◽  
pp. 1-6
Author(s):  
Kristen Vacca ◽  
Asante Shipp-Hilts ◽  
Stephanie Mack ◽  
Yunshu Li ◽  
Millicent Eidson ◽  
...  
Keyword(s):  
New York ◽  
Key Words ◽  
Natural Disaster ◽  
Emergency Preparedness ◽  
New York State ◽  
Hurricane Sandy ◽  
Lessons Learned ◽  
Federal State ◽  
York State ◽  
State And Local

ABSTRACT Objectives: The aim of this study was to assess strengths and challenges experienced by HIV/STD providers in providing care during the response to Hurricane Sandy (Sandy) in New York State, and their recommendations for future preparedness. Methods: A mixed methods approach, including a focus group (n = 3), interviews (n = 3), and survey (n = 31) of HIV/STD providers, was used. Key words identified by means of open coding methodology from collected data were organized into strengths, challenges, and recommendations and then grouped into federal and study-associated preparedness capabilities. Results: Key words were organized into 81 strengths (38.8%), 73 challenges (34.9%), and 55 recommendations (26.3%). Services most interrupted during Sandy were related to HIV/STD outreach and education. While providers reported challenges with external agency communication, the ability to still connect clients to needed resources was reported as a strength. Strengthening partnerships with federal, state, and local agencies was among the major recommendations made by these providers. Conclusions: This study presents unique information about challenges experienced by HIV/STD providers in providing services during a natural disaster and the use of national public health emergency preparedness capabilities to address and overcome those challenges. Lessons learned and recommendations regarding inter-agency communications emerged as an important priority during a natural disaster to minimize or reduce service interruption.

scholarly journals Improved Safety for Molecular Diagnosis of Classical Rabies Viruses by Use of a TaqMan Real-Time Reverse Transcription-PCR "Double Check" Strategy

2010 ◽  
Vol 48 (11) ◽  
pp. 3970-3978 ◽  
Author(s):  
B. Hoffmann ◽  
C. M. Freuling ◽  
P. R. Wakeley ◽  
T. B. Rasmussen ◽  
S. Leech ◽  
...  
Keyword(s):  
Real Time ◽  
Molecular Diagnosis ◽  
Reverse Transcription ◽  
Reverse Transcription Pcr

Abstract WP185: The Risk of Pregnancy-associated Stroke Does not Increase With Age: an Analysis of Claims Data From New York State

Stroke ◽  
2016 ◽  
Vol 47 (suppl_1) ◽  
Author(s):  
Hajere J Gatollari ◽  
Eliza C Miller ◽  
Gloria Too ◽  
Amelia K Boehme ◽  
Anna Colello ◽  
...  
Keyword(s):  
New York ◽  
Pregnant Women ◽  
Older Women ◽  
Post Partum ◽  
New York State ◽  
Vital Statistics ◽  
York State Department ◽  
Limited Information ◽  
York State ◽  
Increased Risk

Introduction: Stroke is a rare but feared complication of pregnancy and the postpartum period. Prior studies have found that older age is associated with increased risk of pregnancy-associated stroke (PAS). It is unclear to what extent pregnancy contributes to this increased risk of PAS. There is limited information on age-specific incidence ratios to assess whether the risk of PAS is comparable to stroke in similarly aged non-pregnant women. Hypothesis: Increased incidence of PAS in older women is attributable to the increasing risk of stroke with age. Methods: We used the 2008-2012 New York State Department of Health (NYS DOH) Statewide Planning and Research Cooperative System (SPARCS) inpatient database to identify women aged 12-50 with ischemic (ICD-9 433, 434, 436), ICH (ICD-9 431), SAH (ICD-9 430), and non-specified PAS, including post-partum stroke (ICD-9 671.7, 674). Population data were obtained from NYS DOH Vital Statistics. Age-specific incidence ratios were calculated for strokes in pregnant or postpartum women compared to non-pregnant women. We used standard age groups considered by obstetricians to be at low, moderate, or high risk for pregnancy complications, including stroke. Results: Of 7099 stroke hospitalizations occurring in women, 386 were in pregnant women. The table shows the incidence of stroke in women stratified by pregnancy status and age group, and age-specific incidence risk ratios associated with pregnancy. Conclusions: Compared to non-pregnant women of the same age, pregnant women appeared to be at similarly increased risk of stroke at all ages, except for those over age 40. These results suggest that pregnancy itself does not further increase the risk of stroke in older women, though they could also reflect a tendency for healthier women to have pregnancies at later ages. More research is needed to investigate the characteristics that may put younger women at increased risk of stroke, compared to their non-pregnant contemporaries.

Management Challenges in Broadbend Networking

2001 ◽  
pp. 189-218
Author(s):  
Murali Venkatesh
Keyword(s):  
New York ◽  
Support Services ◽  
New York State ◽  
Lessons Learned ◽  
Action Research Project ◽  
Bandwidth Management ◽  
York State ◽  
The Public ◽  
K 12 ◽  
Business Sectors

This paper examines the implications of technological discontinuity for organizations in six communities in New York State. From the network manager’s perspective, two broad sets of challenges are presented: technological – including specific issues such as bandwidth management and access and backbone issues, and applications–and those related to acquisition of technical support services. Applications prototyping is presented as a response to the technological challenges. The action research project (and the broader research program of which it is a part) underlying this chapter is focused on the public, nonprofit—including government and healthcare institutions, so-called social sector nonprofits, K-12 and higher education—and small business sectors, but lessons learned are broadly applicable.

Lessons Learned From the New York State Mental Health Response to the September 11, 2001, Attacks

2011 ◽  
Vol 62 (9) ◽  
pp. 1085-1089 ◽  
Author(s):  
Lloyd I. Sederer ◽  
Carol B. Lanzara ◽  
Susan M. Essock ◽  
Sheila A. Donahue ◽  
James L. Stone ◽  
...  
Keyword(s):  
Mental Health ◽  
New York ◽  
New York State ◽  
September 11 ◽  
State Mental Health ◽  
Lessons Learned ◽  
York State ◽  
Health Response ◽  
State Mental

scholarly journals Ending the HIV Epidemic: New York’s Quest to Become the First State to Reduce HIV Prevalence

2020 ◽  
Vol 135 (1_suppl) ◽  
pp. 65S-74S
Author(s):  
Johanne E. Morne ◽  
James M. Tesoriero ◽  
Erika G. Martin ◽  
Guthrie S. Birkhead ◽  
David R. Holtgrave ◽  
...  
Keyword(s):  
New York ◽  
Service Providers ◽  
New York State ◽  
Hiv Prevalence ◽  
Hiv Treatment ◽  
Lessons Learned ◽  
York State ◽  
Key Stakeholders ◽  
Persons Living With Hiv ◽  
Living With Hiv

In 2014, New York State became the first jurisdiction to launch a statewide initiative to end AIDS by reducing the number of persons living with HIV for the first time since effective HIV treatment became available. The Ending the Epidemic (ETE) initiative encompasses (1) identifying and linking undiagnosed persons with HIV to care, (2) retaining persons with HIV in care, and (3) facilitating access to preexposure prophylaxis for persons at risk for acquiring HIV. We used a framework for public health program implementation to describe key characteristics of the ETE initiative, present progress toward 13 ETE target metrics, and identify areas in need of increased programming. We provide evidence suggesting that New York State is on track to end AIDS as an epidemic by the end of 2020. As of 2017, 76% of progress toward our primary ETE target had been achieved. Substantial progress on several additional metrics critical to decreasing HIV prevalence and to improving the health of persons living with HIV had also been achieved. Lessons learned included the following: (1) ETE-based programming should be tailored to each jurisdiction’s unique political and social climate, HIV epidemiology, fiscal resources, and network of HIV service providers; (2) key stakeholders should be involved in developing ETE metrics and setting targets; (3) performance-based measurement and timely communication to key stakeholders in real time are essential; and (4) examining trends in HIV prevention and care metrics is important for developing realistic ETE timelines.

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