scholarly journals Multiplexed Tandem PCR (MT-PCR) Assay Using the Major Piroplasm Surface Protein Gene for the Diagnosis of Theileria orientalis Infection in Cattle

2017 ◽  
Vol 56 (3) ◽  
Author(s):  
Hagos Gebrekidan ◽  
Robin B. Gasser ◽  
Mark A. Stevenson ◽  
Abdul Jabbar
2014 ◽  
Vol 104 (2) ◽  
pp. 195-202 ◽  
Author(s):  
A. Bordbar ◽  
S. Soleimani ◽  
F. Fardid ◽  
M.R. Zolfaghari ◽  
P. Parvizi

AbstractIndividual wild-caught sandflies from Iran were examined for infections of Wolbachia pipientis by targeting the major surface protein gene wsp of this intracellular α-proteobacterium. In total, 638 male and female sandflies were screened, of which 241 were found to be positive for one of three wsp haplotypes. Regardless of geographical origins and habitats, Phlebotomus (Phlebotomus) papatasi and other sandfly species were found to be infected with one common, widespread strain of A-group W. pipientis (Turk 54, GenBank accession EU780683; AY288297). In addition, a new A-group haplotype (Turk07, GenBank accession KC576916) was isolated from Phlebotomus (Paraphlebotomus) mongolensis and Phlebotomus (Pa.) caucasicus, and a new B-group haplotype (AZ2331, GenBank accession JX488735) was isolated from Phlebotomus (Larroussius) perfiliewi. Therefore, Wolbachia was found to occur in at least three of the incriminated vectors of zoonotic cutaneous leishmaniasis and zoonotic visceral leishmaniasis in different geographical regions of Iran. It may provide a new tool for the future control of leishmaniasis.


1988 ◽  
Vol 8 (11) ◽  
pp. 5043-5046
Author(s):  
J P Kile ◽  
H D Love ◽  
C A Hubach ◽  
G A Bannon

The expression of Tetrahymena surface proteins serotype H3 (SerH3) and serotype T (SerT) is under environmental regulation. SerH3 is expressed when cells are incubated between the temperatures of 20 and 35 degrees C, while SerT is expressed when cells are grown at temperatures above 35 degrees C. Using a SerH3 cDNA clone as a hybridization probe, we determined that (i) the SerH3 gene is a member of a multigene family; (ii) most members of this multigene family are variably rearranged during macronuclear development; and (iii) the gene which produces the SerH3 mRNA is reproducibly rearranged during macronuclear development.


2008 ◽  
Vol 9 (2) ◽  
pp. 155 ◽  
Author(s):  
Myung-Soon Ko ◽  
Kyoung-Kap Lee ◽  
Kyu-Kye Hwang ◽  
Byung-Sun Kim ◽  
Gui-Cheol Choi ◽  
...  

2017 ◽  
Vol 59 (1) ◽  
Author(s):  
Suhee Kim ◽  
Do-Hyeon Yu ◽  
Jeong-Byoung Chae ◽  
Kyoung-Seong Choi ◽  
Hyeon-Cheol Kim ◽  
...  

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