scholarly journals Molecular Epidemiological Survey of Benign Theileria Parasites of Cattle in Japan: Detection of a New Type of Major Piroplasm Surface Protein Gene

2004 ◽  
Vol 66 (3) ◽  
pp. 251-256 ◽  
Author(s):  
Jung-Yeon KIM ◽  
Naoaki YOKOYAMA ◽  
Sanjay KUMAR ◽  
Noboru INOUE ◽  
Toshiaki YAMAGUCHI ◽  
...  
Keyword(s):  
Protein Gene ◽  
Surface Protein ◽  
Epidemiological Survey ◽  
New Type ◽  
Surface Protein Gene ◽  
Major Piroplasm Surface Protein

Three strains of Wolbachia pipientis and high rates of infection in Iranian sandfly species

2014 ◽  
Vol 104 (2) ◽  
pp. 195-202 ◽  
Author(s):  
A. Bordbar ◽  
S. Soleimani ◽  
F. Fardid ◽  
M.R. Zolfaghari ◽  
P. Parvizi
Keyword(s):  
Protein Gene ◽  
Surface Protein ◽  
Male And Female ◽  
Wolbachia Pipientis ◽  
Major Surface Protein ◽  
Zoonotic Cutaneous Leishmaniasis ◽  
Geographical Regions ◽  
Surface Protein Gene ◽  
Major Surface ◽  
Zoonotic Visceral Leishmaniasis

AbstractIndividual wild-caught sandflies from Iran were examined for infections of Wolbachia pipientis by targeting the major surface protein gene wsp of this intracellular α-proteobacterium. In total, 638 male and female sandflies were screened, of which 241 were found to be positive for one of three wsp haplotypes. Regardless of geographical origins and habitats, Phlebotomus (Phlebotomus) papatasi and other sandfly species were found to be infected with one common, widespread strain of A-group W. pipientis (Turk 54, GenBank accession EU780683; AY288297). In addition, a new A-group haplotype (Turk07, GenBank accession KC576916) was isolated from Phlebotomus (Paraphlebotomus) mongolensis and Phlebotomus (Pa.) caucasicus, and a new B-group haplotype (AZ2331, GenBank accession JX488735) was isolated from Phlebotomus (Larroussius) perfiliewi. Therefore, Wolbachia was found to occur in at least three of the incriminated vectors of zoonotic cutaneous leishmaniasis and zoonotic visceral leishmaniasis in different geographical regions of Iran. It may provide a new tool for the future control of leishmaniasis.

Reproducible and variable rearrangements of a Tetrahymena thermophila surface protein gene family occur during macronuclear development

1988 ◽  
Vol 8 (11) ◽  
pp. 5043-5046
Author(s):  
J P Kile ◽  
H D Love ◽  
C A Hubach ◽  
G A Bannon
Keyword(s):  
Environmental Regulation ◽  
Cdna Clone ◽  
Multigene Family ◽  
Protein Gene ◽  
Tetrahymena Thermophila ◽  
Surface Protein ◽  
Surface Proteins ◽  
Hybridization Probe ◽  
Macronuclear Development ◽  
Surface Protein Gene

The expression of Tetrahymena surface proteins serotype H3 (SerH3) and serotype T (SerT) is under environmental regulation. SerH3 is expressed when cells are incubated between the temperatures of 20 and 35 degrees C, while SerT is expressed when cells are grown at temperatures above 35 degrees C. Using a SerH3 cDNA clone as a hybridization probe, we determined that (i) the SerH3 gene is a member of a multigene family; (ii) most members of this multigene family are variably rearranged during macronuclear development; and (iii) the gene which produces the SerH3 mRNA is reproducibly rearranged during macronuclear development.

scholarly journals Antigenic diversity ofTheileriamajor piroplasm surface protein gene in Jeju black cattle

2008 ◽  
Vol 9 (2) ◽  
pp. 155 ◽  
Author(s):  
Myung-Soon Ko ◽  
Kyoung-Kap Lee ◽  
Kyu-Kye Hwang ◽  
Byung-Sun Kim ◽  
Gui-Cheol Choi ◽  
...  
Keyword(s):  
Protein Gene ◽  
Surface Protein ◽  
Antigenic Diversity ◽  
Surface Protein Gene ◽  
Jeju Black Cattle

scholarly journals Enterococcal Surface Protein Esp Is Important for Biofilm Formation of Enterococcus faecium E1162

2007 ◽  
Vol 189 (22) ◽  
pp. 8233-8240 ◽  
Author(s):  
Esther Heikens ◽  
Marc J. M. Bonten ◽  
Rob J. L. Willems
Keyword(s):  
Enterococcus Faecium ◽  
Biofilm Formation ◽  
Deletion Mutant ◽  
Wild Type Strain ◽  
Protein Gene ◽  
Surface Protein ◽  
Essential Factor ◽  
Wild Type ◽  
Surface Protein Gene ◽  
Multiple Antibiotics

ABSTRACT Enterococci have emerged as important nosocomial pathogens with resistance to multiple antibiotics. Adhesion to abiotic materials and biofilm formation on medical devices are considered important virulence properties. A single clonal lineage of Enterococcus faecium, complex 17 (CC17), appears to be a successful nosocomial pathogen, and most CC17 isolates harbor the enterococcal surface protein gene, esp. In this study, we constructed an esp insertion-deletion mutant in a clinical E. faecium CC17 isolate. In addition, initial adherence and biofilm assays were performed. Compared to the wild-type strain, the esp insertion-deletion mutant no longer produced Esp on the cell surface and had significantly lower initial adherence to polystyrene and significantly less biofilm formation, resulting in levels of biofilm comparable to those of an esp-negative isolate. Capacities for initial adherence and biofilm formation were restored in the insertion-deletion mutant by in trans complementation with esp. These results identify Esp as the first documented determinant in E. faecium CC17 with an important role in biofilm formation, which is an essential factor in infection pathogenesis.

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