scholarly journals Indirect fluorescent antibody procedure for the rapid detection and identification of Bacteroides and Fusobacterium in clinical specimens

1975 ◽  
Vol 2 (4) ◽  
pp. 337-344
Author(s):  
L R Stauffer ◽  
E O Hill ◽  
J W Holland ◽  
W A Altemeier
Keyword(s):  
Rapid Detection ◽  
Fluorescent Antibody ◽  
Indirect Fluorescent Antibody ◽  
Clinical Specimens ◽  
Detection And Identification ◽  
Cellular Debris ◽  
Practical Tool ◽  
Human Infections ◽  
Fusobacterium Species

An indirect fluorescent antibody (IFA) technique was evaluated as a procedure for rapid detection and identification of members of the Bacteroidaceae. Antisera were prepared against 31 members of this family, including species of Bacteroides and Fusobacterium commonly isolated from human infections. The antisera had demonstrated species and/or subspecies specificity. Thirty clinical specimens were studied. Of 13 specimens yielding Bacteroidaceae, for which antisera were available, 23 were presumptively diagnosed by IFA to contain subspecies of B. fragilis and/or Fusobacterium species. Of 17 specimens yielding negative culture results, two were positive by IFA on direct smear. Frequently the in vivo morphology of cells detected in direct smears by this procedure closely mimicked that of cellular debris, tissue cells, and leukocytes. Polyvalent antisera pools facilitated use of the IFA procedure as a practical tool for rapid diagnosis of infections involving the Bacteroidaceae.

The blocking of human antibody-dependent, eosinophil-mediated killing ofSchistosoma mansonischistosomula by monoclonal antibodies which cross-react with a polysaccharide-containing egg antigen

Parasitology ◽  
1987 ◽  
Vol 94 (2) ◽  
pp. 269-280 ◽  
Author(s):  
D. W. Dunne ◽  
Q. D. Bickle ◽  
A. E. Butter Worth ◽  
B. A. Richardson
Keyword(s):  
Monoclonal Antibody ◽  
Monoclonal Antibodies ◽  
Fluorescent Antibody ◽  
Human Infection ◽  
Human Antibody ◽  
Indirect Fluorescent Antibody ◽  
Antibody Assay ◽  
Antigen Preparation ◽  
Human Infections ◽  
Egg Antigen

SUMMARYThree IgM monoclonal antibodies, M22G11P, M7B7 and M22B3G, which reacted with the surfaceof Schistosoma mansonischistosomula in an indirect fluorescent antibody assay, were found to recognize a polysaccharide-containing egg antigen previously designated K3. The monoclonal antibodies and a monospecific rabbit anti-K3serum also recognized a crossreacting antigen in a crude cercarial antigen preparation. In an eosinophil-mediated schistosomulum killing assay, all three monoclonal antibodies significantly inhibited the level of killing produced by human infection serum. An IgG3 monoclonal antibody, M22C1C, which also recognized the egg antigen K3, did not inhibit eosinophil-mediated killing. However, when lower concentrations of human serum were used in the assay, this monoclonal antibody significantly enhanced the level of killing, despite having no capacity to induce eosinophil-mediated damage in the absence of human infection serum. On the basis of these and other results we suggest the possibility that antibodies toS. mansoniegg antigens which cross-react with the surface of the early post-penetration schistosomulum may influence the effective expression of antibody-dependent, eosinophil-mediated effector mechanisms in human infections.

Indirect Fluorescent Antibody Technique for Detection of RM Bacterium of Rainbow Trout (Salmo gairdneri)

1974 ◽  
Vol 31 (12) ◽  
pp. 1957-1959 ◽  
Author(s):  
G. R. Johnson ◽  
G. Wobeser ◽  
B. T. Rouse
Keyword(s):  
Rainbow Trout ◽  
Rapid Detection ◽  
Fluorescent Antibody ◽  
Salmo Gairdneri ◽  
Fluorescent Antibody Technique ◽  
Indirect Fluorescent Antibody ◽  
Antibody Technique

An indirect fluorescent antibody technique was evaluated as a method for the rapid detection of RM bacterium of rainbow trout (Salmo gairdneri). Antibody produced by inoculating rabbits with washed RM bacterium antigen was combined with commercially prepared fluorescein-labelled antiglobulin. The technique demonstrated strong specificity for the RM bacterium in washed and broth cultures, and in kidney smears from experimentally infected rainbow trout.

Solid-Phase Radioimmunossay for Rapid Detection and Identification of Western Equine Encephalomyelitis Virus

1976 ◽  
Vol 4 (4) ◽  
pp. 382-383
Author(s):  
Neil H. Levitt ◽  
Helen V. Miller ◽  
Gerald A. Eddy
Keyword(s):  
Rapid Detection ◽  
Solid Phase ◽  
Clinical Specimens ◽  
Detection And Identification ◽  
Encephalomyelitis Virus ◽  
Solid Phase Radioimmunoassay ◽  
Western Equine Encephalomyelitis Virus

A solid-phase radioimmunoassay technique was adapted for the rapid detection and identification of western equine encephalomyelitis virus in clinical specimens.

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