Rapid detection of bovine viral diarrhea virus by using RNA extracted directly from assorted specimens and a one-tube reverse transcription PCR assay.

1995 ◽  
Vol 33 (2) ◽  
pp. 287-291 ◽  
Author(s):  
A L Hamel ◽  
M D Wasylyshen ◽  
G P Nayar
Keyword(s):  
Bovine Viral Diarrhea Virus ◽  
Reverse Transcription ◽  
Rapid Detection ◽  
Bovine Viral Diarrhea ◽  
Pcr Assay ◽  
Diarrhea Virus ◽  
Viral Diarrhea ◽  
Reverse Transcription Pcr ◽  
Viral Diarrhea Virus

scholarly journals Multiplex real-time RT-PCR assay for bovine viral diarrhea virus type 1, type 2 and HoBi-like pestivirus

2016 ◽  
Vol 229 ◽  
pp. 1-7 ◽  
Author(s):  
Viviana Mari ◽  
Michele Losurdo ◽  
Maria Stella Lucente ◽  
Eleonora Lorusso ◽  
Gabriella Elia ◽  
...  
Keyword(s):  
Real Time ◽  
Bovine Viral Diarrhea Virus ◽  
Bovine Viral Diarrhea ◽  
Rt Pcr ◽  
Pcr Assay ◽  
Diarrhea Virus ◽  
Viral Diarrhea ◽  
Viral Diarrhea Virus

Bovine viral diarrhea virus subgenotype 1a in a mummified fetus from a Brazilian dairy cattle herd

2021 ◽  
pp. 104063872110220
Author(s):  
Juliana T. T. Fritzen ◽  
Arthur B. Morettin ◽  
Elis Lorenzetti ◽  
Alice F. Alfieri ◽  
Amauri A. Alfieri
Keyword(s):  
Dairy Cattle ◽  
Bovine Viral Diarrhea Virus ◽  
Cattle Herd ◽  
Bovine Viral Diarrhea ◽  
Rt Pcr ◽  
Pcr Assay ◽  
Diarrhea Virus ◽  
Viral Diarrhea ◽  
Dairy Cattle Herd ◽  
Viral Diarrhea Virus

We describe the molecular analysis of a wild-type field strain of bovine viral diarrhea virus (BVDV) identified in a mummified fetus from a small Brazilian dairy cattle herd. Nucleic acids extracted from samples of the lung, liver, heart, spleen, and kidney were tested by PCR assays for bovine alphaherpesvirus 1, Neospora caninum, Leptospira spp., Histophilus somni, and Brucella abortus, a nested PCR assay for Mycoplasma bovigenitalium and Ureaplasma diversum, and a RT-PCR assay for BVDV. Amplicons were only obtained in the RT-PCR assay for the partial amplification of the BVDV 5′UTR (288 bp) in kidney and spleen samples and the Npro (438 bp) gene in the kidney sample. Nucleotide sequencing of the amplified products and phylogenetic analyses based on the 2 BVDV genomic regions enabled the BVDV strain to be classified as subgenotype 1a.

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