scholarly journals PCR amplicon restriction endonuclease analysis of the chromosomal dhps gene of Neisseria meningitidis: a method for studying spread of the disease-causing strain in contacts of patients with meningococcal disease.

1995 ◽  
Vol 33 (5) ◽  
pp. 1174-1179 ◽  
Author(s):  
B E Kristiansen ◽  
C Fermér ◽  
A Jenkins ◽  
E Ask ◽  
G Swedberg ◽  
...  
Keyword(s):  
Neisseria Meningitidis ◽  
Restriction Endonuclease ◽  
Meningococcal Disease ◽  
Restriction Endonuclease Analysis ◽  
Dhps Gene ◽  
Endonuclease Analysis

scholarly journals Clonal Distribution of Invasive Neisseria meningitidis Isolates from the Norwegian County of Telemark, 1987 to 1995

1998 ◽  
Vol 36 (9) ◽  
pp. 2623-2628 ◽  
Author(s):  
Randi Kersten Aakre ◽  
Andrew Jenkins ◽  
Bjørn-Erik Kristiansen ◽  
L. Oddvar Frøholm
Keyword(s):  
Neisseria Meningitidis ◽  
Restriction Endonuclease ◽  
Meningococcal Disease ◽  
Restriction Endonuclease Analysis ◽  
Serological Analysis ◽  
Chromosomal Dna ◽  
Phenotypic Characteristics ◽  
Class 1 ◽  
Clonal Distribution ◽  
Endonuclease Analysis

Forty-two Neisseria meningitidis isolates were obtained from patients with meningococcal disease in the Norwegian county of Telemark (January 1987 to March 1995), and all were compared by PCR amplicon restriction endonuclease analysis (PCR-AREA) of thedhps gene, chromosomal DNA fingerprinting, and serological analysis. PCR-AREA divided the isolates into 11 classes, of which 4, comprising 15, 8, 6, and 2 isolates, were clonal while the remaining 8 classes were genetically heterogeneous or contained only 1 isolate. Three of the four clonal classes could be tentatively equated with recognized epidemic clones (ET5, ET37, and cluster A4) on the basis of their phenotypic characteristics, while the remaining clone appears to be new. There were significant differences in the geographical distribution of clones, with class 1 (ET5-like) isolates significantly overrepresented in rural parts of Telemark. Class 1 (ET5-like) isolates occurred throughout the study period and were dominant in 1987. Class 2 (ET37-like) isolates occurred from 1988 to 1992, and class 3 isolates (with no recognizable ET affinities) were found only in 1991 and 1992.

scholarly journals Continuing Mycobacterium bovis transmission from animals to humans in New Zealand

2006 ◽  
Vol 134 (5) ◽  
pp. 1068-1073 ◽  
Author(s):  
M. G. BAKER ◽  
L. D. LOPEZ ◽  
M. C. CANNON ◽  
G. W. DE LISLE ◽  
D. M. COLLINS
Keyword(s):  
New Zealand ◽  
Restriction Endonuclease ◽  
Mycobacterium Bovis ◽  
Restriction Endonuclease Analysis ◽  
Domestic Animals ◽  
Human Infection ◽  
Potential Contribution ◽  
Large Reservoir ◽  
Animal Reservoirs ◽  
Endonuclease Analysis

New Zealand has a large reservoir of Mycobacterium bovis infection in wild and farmed animals. This study aimed to assess the extent of human infection with this organism and the potential contribution of these animal sources. Combined epidemiological and laboratory investigation of human tuberculosis cases over the period 1995–2002 showed that M. bovis accounted for 2·7% (54/1997) of laboratory-confirmed human tuberculosis cases, a rate of 0·2/100000 population. M. bovis isolates from humans (23) were typed using restriction endonuclease analysis (REA) and compared with isolates from wild and domestic animals (2600). Fourteen (61%) of the human isolates had REA patterns that were identical to patterns for isolates from cattle, deer, possums, ferrets, pigs, and occasionally cats. These results suggest a low level of ongoing M. bovis transmission from animal reservoirs to humans in New Zealand.

Transfusion transmission of cytomegalovirus confirmed by restriction endonuclease analysis

1985 ◽  
Vol 107 (6) ◽  
pp. 953-956 ◽  
Author(s):  
Mark D. Tolpin ◽  
John A. Stewart ◽  
Dora Warren ◽  
Benjamin A. Mojica ◽  
Mary A. Collins ◽  
...  
Keyword(s):  
Restriction Endonuclease ◽  
Restriction Endonuclease Analysis ◽  
Endonuclease Analysis
Sign in / Sign up

Export Citation Format

Share Document