Role of Genotypic Analysis of the Thymidine Kinase Gene of Herpes Simplex Virus for Determination of Neurovirulence and Resistance to Acyclovir

2000 ◽  
Vol 38 (3) ◽  
pp. 1311-1311
Author(s):  
N. Y. Lee ◽  
Y.-W. Tang ◽  
M. J. Espy ◽  
C. P. Kolbert ◽  
P. N. Rys ◽  
...  
Keyword(s):  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Thymidine Kinase ◽  
Thymidine Kinase Gene ◽  
Kinase Gene ◽  
Genotypic Analysis ◽  
Simplex Virus

scholarly journals Role of Genotypic Analysis of the Thymidine Kinase Gene of Herpes Simplex Virus for Determination of Neurovirulence and Resistance to Acyclovir

1999 ◽  
Vol 37 (10) ◽  
pp. 3171-3174 ◽  
Author(s):  
N. Y. Lee ◽  
Y.-W. Tang ◽  
M. J. Espy ◽  
C. P. Kolbert ◽  
P. N. Rys ◽  
...  
Keyword(s):  
Herpes Simplex Virus ◽  
Amino Acid ◽  
Herpes Simplex ◽  
Thymidine Kinase ◽  
Kinase Gene ◽  
Genotypic Analysis ◽  
Simplex Virus ◽  
Hsv 1

Mutations in the thymidine kinase (TK) gene of herpes simplex virus (HSV) have been associated with resistance to acyclovir (ACY) and possible recognition of neurotropic strains. We sequenced a 335-bp segment of the TK gene to determine the frequency of mutations in HSV strains recovered from dermal, genital, and cerebrospinal fluid (CSF) specimens (n = 200; 102 HSV type 1 [HSV-1] 98 HSV-2 strains). Four polymorphic sites were detected in HSV-1 strains; C513T, A528G, C575T, and C672T. Among the polymorphisms, only C575T resulted in a change of amino acid sequence (residue 192, Ala→Val). For HSV-2 strains, only one polymorphism (G420T) which resulted in an amino acid substitution (residue 139, Leu→Phe) was detected. Phenotypic determination of resistance to ACY by a plaque reduction assay of 48 HSV isolates was not correlated with the sequence results of 11 strains in that 7 of these with genotypic polymorphisms were susceptible to the drug in vitro. In addition, of 32 ACY-resistant HSV strains, 28 (87.5%) had no polymorphisms detected in the 335-bp amplicon of the TK gene. There was no statistical difference in the frequency of polymorphisms according to the source of the specimens. We conclude that the detection of nucleic acid polymorphisms in a previously implicated 335-bp segment of the TK gene cannot be interpreted as indicative of either ACY resistance or neurotropism of HSV strains from dermal, genital, and CSF sources.

scholarly journals Hybrid plasmids containing an active thymidine kinase gene of Herpes simplex virus 1

1979 ◽  
Vol 7 (4) ◽  
pp. 859-878 ◽  
Author(s):  
N.M. Wilkie ◽  
J.B. Clements ◽  
W. Boll ◽  
N. Mantei ◽  
D. Lonsdale ◽  
...  
Keyword(s):  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Thymidine Kinase ◽  
Thymidine Kinase Gene ◽  
Herpes Simplex Virus 1 ◽  
Kinase Gene ◽  
Simplex Virus

Adenoviral-Mediated Herpes Simplex Virus Thymidine Kinase Gene Transfer

Pancreas ◽  
1997 ◽  
Vol 15 (1) ◽  
pp. 25-34 ◽  
Author(s):  
Andreas Block ◽  
Shu-Hsia Chen ◽  
Ken-Ichiro Kosai ◽  
Milton Finegold ◽  
Savio L. C. Woo
Keyword(s):  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Gene Transfer ◽  
Thymidine Kinase ◽  
Thymidine Kinase Gene ◽  
Kinase Gene ◽  
Simplex Virus

scholarly journals Adenoviral-Mediated Herpes Simplex Virus-Thymidine Kinase Gene Transfer in Vivo for Treatment of Experimental Human Melanoma

1996 ◽  
Vol 106 (6) ◽  
pp. 1163-1168 ◽  
Author(s):  
Bernd Bonnekoh ◽  
David A. Greenhalgh ◽  
Donnie S. Bundman ◽  
Ken-ichiro Kosai ◽  
Shu-Hsia Chen ◽  
...  
Keyword(s):  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Gene Transfer ◽  
Thymidine Kinase ◽  
Human Melanoma ◽  
Thymidine Kinase Gene ◽  
Kinase Gene ◽  
Simplex Virus

scholarly journals Expression of Porcine FSHβ Subunit Promoter-driven Herpes Simplex Virus Thymidine Kinase Gene in Transgenic Rats

2007 ◽  
Vol 53 (2) ◽  
pp. 201-209 ◽  
Author(s):  
Li-yi CAI ◽  
Takako KATO ◽  
Kazumi ITO ◽  
Michie NAKAYAMA ◽  
Takao SUSA ◽  
...  
Keyword(s):  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Thymidine Kinase ◽  
Thymidine Kinase Gene ◽  
Transgenic Rats ◽  
Kinase Gene ◽  
Simplex Virus

scholarly journals Severe hepatic dysfunction after adenovirus-mediated transfer of the herpes simplex virus thymidine kinase gene and ganciclovir administration

Gene Therapy ◽  
1998 ◽  
Vol 5 (4) ◽  
pp. 451-458 ◽  
Author(s):  
MM van der Eb ◽  
SJ Cramer ◽  
Y Vergouwe ◽  
FHE Schagen ◽  
J H J M van Krieken ◽  
...  
Keyword(s):  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Thymidine Kinase ◽  
Hepatic Dysfunction ◽  
Thymidine Kinase Gene ◽  
Kinase Gene ◽  
Simplex Virus
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