scholarly journals A Novel Multiresistant Streptococcus pneumoniae Serogroup 19 Clone from Washington State Identified by Pulsed-Field Gel Electrophoresis and Restriction Fragment Length Patterns

2000 ◽  
Vol 38 (4) ◽  
pp. 1575-1580 ◽  
Author(s):  
Vicki A. Luna ◽  
Daniel B. Jernigan ◽  
Alan Tice ◽  
James D. Kellner ◽  
Marilyn C. Roberts
Keyword(s):  
Streptococcus Pneumoniae ◽  
Restriction Fragment ◽  
Fragment Length ◽  
Gel Electrophoresis ◽  
Washington State ◽  
Pulsed Field Gel Electrophoresis ◽  
Erythromycin Resistance ◽  
Eastern Canada ◽  
Pulsed Field ◽  
Restriction Fragment Length

In 1997, a cluster of multiresistant invasive serogroup 19 pneumococcus infections, including two fatalities, was reported in Washington State. Further investigation identified other cases. Fourteen Washington Streptococcus pneumoniae isolates, four from Alaska, and eight isolates from eastern Canada with reduced penicillin susceptibility (MIC of ≥1 μg/ml) were included in the study. Pulsed-field gel electrophoresis (PFGE) with ApaI,SacII, and SmaI restriction enzymes and IS1167 and mef restriction fragment length polymorphism (RFLP) pattern analysis were performed. Twenty of the 26 isolates had identical or related PFGE patterns, with two or all three enzymes, and identical or related IS1167 RFLP patterns, indicating that they were genetically related. These 20 isolates contained the mef gene conferring erythromycin resistance and had identical mef RFLP patterns. The PFGE and RFLP patterns were distinct from those of six multiresistant clones previously described and suggest that a new multiresistant clone has appeared in Washington, Alaska, and eastern Canada. This newly characterized clone should be included in the Pneumococcal Molecular Epidemiology Network.

scholarly journals Serotype 19F Multiresistant Pneumococcal Clone Harboring Two Erythromycin Resistance Determinants [erm(B) and mef(A)] in South Africa

2001 ◽  
Vol 45 (5) ◽  
pp. 1595-1598 ◽  
Author(s):  
Lesley McGee ◽  
Keith P. Klugman ◽  
Avril Wasas ◽  
Thora Capper ◽  
Adrian Brink
Keyword(s):  
South Africa ◽  
Streptococcus Pneumoniae ◽  
Private Sector ◽  
Dna Fingerprinting ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Erythromycin Resistance ◽  
Pulsed Field ◽  
Resistance Determinants ◽  
Genes Encoding

ABSTRACT One hundred eighteen erythromycin-resistant Streptococcus pneumoniae (ERSP) strains (MICs of ≥0.5 μg/ml) from five laboratories serving the private sector in South Africa were analyzed for the genes encoding resistance to macrolides. Sixty-seven ERSP strains (56.8%) contained the erm(B) gene, and 15 isolates (12.7%) contained the mef(A) gene. Thirty-six isolates (30.5%) harbored both the erm(B) and mef(A) genes and were highly resistant to erythromycin and clindamycin. DNA fingerprinting by BOX-PCR and pulsed-field gel electrophoresis identified 83% of these strains as belonging to a single multiresistant serotype 19F clone.

scholarly journals Extensive deletions and insertions in different MHC supratypes detected by pusled field gel electrophoresis.

1988 ◽  
Vol 168 (3) ◽  
pp. 933-940 ◽  
Author(s):  
K Tokunaga ◽  
G Saueracker ◽  
P H Kay ◽  
F T Christiansen ◽  
R Anand ◽  
...  
Keyword(s):  
Restriction Fragment ◽  
Fragment Length ◽  
Gel Electrophoresis ◽  
Genomic Dna ◽  
Genomic Organization ◽  
Restriction Endonucleases ◽  
Band Intensity ◽  
Pulsed Field ◽  
Pulsed Field Electrophoresis ◽  
Restriction Fragment Length

The genomic organization of the human MHC was examined in multiple examples of six different supratypes using pulsed field electrophoresis (PFGE) after digestion of genomic DNA with infrequency cutting restriction endonucleases. Differences in restriction fragment length and band intensity were shown to be specific for each supratype. Mapping of the MHC revealed that each supratype contains previously undescribed deletions and insertions between HLA B and DQ regions.

scholarly journals Phenotypic and Molecular Analysis of Penicillin-Nonsusceptible Streptococcus pneumoniae Isolates in Poland

2006 ◽  
Vol 51 (1) ◽  
pp. 40-47 ◽  
Author(s):  
Ewa Sadowy ◽  
Radosław Izdebski ◽  
Anna Skoczyńska ◽  
Paweł Grzesiowski ◽  
Marek Gniadkowski ◽  
...  
Keyword(s):  
Streptococcus Pneumoniae ◽  
Molecular Analysis ◽  
Gel Electrophoresis ◽  
Multilocus Sequence Typing ◽  
Susceptibility Testing ◽  
Bacterial Pathogen ◽  
Pulsed Field Gel Electrophoresis ◽  
Pulsed Field ◽  
Related Clone ◽  
Sequence Types

ABSTRACT β-Lactams are the drugs of choice for the treatment of infections caused by the important bacterial pathogen Streptococcus pneumoniae. The recent growth of resistance of this organism to penicillin observed worldwide is of the highest concern. In this study, using 887 surveillance pneumococcal isolates recovered in Poland from 1998 to 2002, we observed the increase in penicillin nonsusceptibility from 8.7% to 20.3%. All of the 109 penicillin-nonsusceptible S. pneumoniae (PNSP) isolates identified, together with 22 archival PNSP isolates from 1995 to 1997, were subsequently analyzed by susceptibility testing, serotyping, profiling of pbp genes, pulsed-field gel electrophoresis, and multilocus sequence typing (MLST). Four predominant serotypes, serotypes 6B, 9V, 14, and 23F, characterized 85.5% of the isolates. MLST revealed the presence of 34 sequence types, 15 of which were novel types. Representatives of seven multiresistant international clones (Spain23F-1, Spain6B-2, Spain9V-3, Taiwan23F-15, Poland23F-16, Poland6B-20, and Sweden15A-25) or their closely related variants comprised the majority of the study isolates. The spread of Spain9V-3 and its related clone of serotype 14/ST143 has remarkably contributed to the recent increase in penicillin resistance in pneumococci in the country.

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