scholarly journals Simultaneous Detection of Entamoeba histolytica, Giardia lamblia, and Cryptosporidium parvum in Fecal Samples by Using Multiplex Real-Time PCR

2004 ◽  
Vol 42 (3) ◽  
pp. 1220-1223 ◽  
Author(s):  
J. J. Verweij ◽  
R. A. Blange ◽  
K. Templeton ◽  
J. Schinkel ◽  
E. A. T. Brienen ◽  
...  
Keyword(s):  
Real Time ◽  
Entamoeba Histolytica ◽  
Cryptosporidium Parvum ◽  
Real Time Pcr ◽  
Giardia Lamblia ◽  
Simultaneous Detection ◽  
Fecal Samples

Detecting Cryptosporidium parvum and Giardia lamblia by coagulation concentration and real-time PCR quantification

2012 ◽  
Vol 7 (1) ◽  
pp. 49-54 ◽  
Author(s):  
Huining Zhang ◽  
Xiaohu Zhang ◽  
Shuting Zhang ◽  
Bo Wei ◽  
Qipei Jiang ◽  
...  
Keyword(s):  
Real Time ◽  
Cryptosporidium Parvum ◽  
Real Time Pcr ◽  
Giardia Lamblia

Detection of Cryptosporidium parvum and Giardia lamblia in Water Supply by Quantitative Real-Time PCR

2012 ◽  
Vol 518-523 ◽  
pp. 3707-3711
Author(s):  
Peng Peng ◽  
Rui Bao Jia ◽  
Yu Mei Liu ◽  
Li Li
Keyword(s):  
Water Supply ◽  
Real Time ◽  
Cryptosporidium Parvum ◽  
Real Time Pcr ◽  
Giardia Lamblia ◽  
Pcr Primers ◽  
Quantitative Real Time Pcr ◽  
Rt Pcr ◽  
High Recovery ◽  
Pcr Method

Cryptosporidium parvum and Giardia lamblia are common pathogenic protozoa in water, which pose high risk to drinking water supply. In the present study, detection of C. parvum and G. lamblia was performed by quantitative real-time PCR (RT-PCR). Pairs of PCR primers were evaluated for the detection specificity to pathogenic C. parvum and G. lamblia. The recovery of the RT-PCR detection procedure was examined and high recovery rates (i.e., more than 45％ for C. parvum and more than 50％ for G. lamblia ) were achieved. The RT-PCR method was used to detect C. parvum and G. lamblia in a secondary water supply. The results indicated the potential application of the quantitative RT- PCR method in detection of C. parvum and G. lamblia in water supply.

scholarly journals Real-Time PCR for Detection and Differentiation of Entamoeba histolytica and Entamoeba dispar in Fecal Samples

2002 ◽  
Vol 40 (12) ◽  
pp. 4413-4417 ◽  
Author(s):  
J. Blessmann ◽  
H. Buss ◽  
P. A. T. Nu ◽  
B. T. Dinh ◽  
Q. T. V. Ngo ◽  
...  
Keyword(s):  
Real Time ◽  
Entamoeba Histolytica ◽  
Real Time Pcr ◽  
Entamoeba Dispar ◽  
Fecal Samples

Real-time PCR assay targeting the actin gene for the detection of Cryptosporidium parvum in calf fecal samples

2011 ◽  
Vol 110 (5) ◽  
pp. 1741-1745 ◽  
Author(s):  
Camila G. Homem ◽  
Alex A. Nakamura ◽  
Deuvânia C. Silva ◽  
Weslen F. P. Teixeira ◽  
Willian M. D. Coelho ◽  
...  
Keyword(s):  
Real Time ◽  
Cryptosporidium Parvum ◽  
Real Time Pcr ◽  
Actin Gene ◽  
Pcr Assay ◽  
Fecal Samples
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