scholarly journals Comparison of the Directigen Flu A+B Membrane Enzyme Immunoassay with Viral Culture for Rapid Detection of Influenza A and B Viruses in Respiratory Specimens

2004 ◽  
Vol 42 (8) ◽  
pp. 3707-3710 ◽  
Author(s):  
A. C. Cazacu ◽  
S. E. Chung ◽  
J. Greer ◽  
G. J. Demmler
1992 ◽  
Vol 40 (1) ◽  
pp. 77-84 ◽  
Author(s):  
Gilles Duverlie ◽  
Laurent Houbart ◽  
Bertrand Visse ◽  
Jean-Jacques Chomel ◽  
Jean-Claude Manuguerra ◽  
...  

2004 ◽  
Vol 78 (7) ◽  
pp. 597-603 ◽  
Author(s):  
Keiko MITAMURA ◽  
Masahiko YAMAZAKI ◽  
Masataka ICHIKAWA ◽  
Kazuhiro KIMURA ◽  
Chiharu KAWAKAMI ◽  
...  

1994 ◽  
Vol 139 (3-4) ◽  
pp. 439-444 ◽  
Author(s):  
B. Schweiger ◽  
I. Lange ◽  
R. Heckler ◽  
H. Willers ◽  
E. Schreier

2016 ◽  
Vol 54 (7) ◽  
pp. 1820-1825 ◽  
Author(s):  
Jonathan H. K. Chen ◽  
Ho-Yin Lam ◽  
Cyril C. Y. Yip ◽  
Sally C. Y. Wong ◽  
Jasper F. W. Chan ◽  
...  

A broad range of viral and bacterial pathogens can cause acute respiratory tract infection. For rapid detection of a broad respiratory pathogen spectrum, multiplex real-time PCR is ideal. This study evaluated the performance of the new Luminex NxTAG Respiratory Pathogen Panel (NxTAG-RPP) in comparison with the BioFire FilmArray Respiratory Panel (FA-RP) or singleplex real-time PCR as reference. A total of 284 clinical respiratory specimens and 3 influenza A/H7N9 viral culture samples were tested. All clinical specimens were processed and analyzed in parallel using NxTAG-RPP and the reference standard method. The H7N9 viral culture samples were tested using NxTAG-RPP only. Overall, the NxTAG-RPP demonstrated ≥93% sensitivity and specificity for all respiratory targets except human coronavirus OC43 (HCoV-OC43) and HCoV-HKU1. The H7N9 virus was detected by the influenza A virus matrix gene target, while other influenza A virus subtyping gene targets in the panel remained negative. Complete concordance between NxTAG-RPP and FA-RP was observed in 98.8% (318/322) of positive results (kappa = 0.92). Substantial agreement was found for most respiratory targets, but significant differences were observed in human metapneumovirus (P= 0.001) and parainfluenza virus type 3 (P= 0.031). NxTAG-RPP has a higher sample throughput than FA-RP (96 samples versus 1 sample per run) while the turnaround times for NxTAG-RPP and FA-RP were 5 h (up to 96 samples) and 1 h (for one sample), respectively. Overall, NxTAG-RPP demonstrated good diagnostic performance for most respiratory pathogens. The high sample throughput with reasonable turnaround time of this new assay makes it a suitable multiplex platform for routine screening of respiratory specimens in hospital-based laboratories.


2010 ◽  
Vol 59 (6) ◽  
pp. 713-717 ◽  
Author(s):  
Tina Ganzenmueller ◽  
Jeanette Kluba ◽  
Birgit Hilfrich ◽  
Wolfram Puppe ◽  
Willem Verhagen ◽  
...  

Although infections with the novel pandemic 2009 influenza A (H1N1) virus (A/H1N1/2009) appeared to be relatively mild during the first summer of circulation (‘off season’), there has been significant morbidity and hospitalization and several fatal cases. Thus, rapid detection of A/H1N1/2009 is crucial for efficient treatment and infection control measures. In contrast to seasonal influenza, where point-of-care (POC) rapid antigen tests and direct fluorescent antibody (DFA) staining ensure rapid detection, diagnosis of A/H1N1/2009 has so far been based on RT-PCR. This study retrospectively compared the performance of the Quidel QuickVue POC test, DFA staining and virus isolation with that of RT-PCR for A/H1N1/2009 detection in 526 respiratory specimens collected during the first wave of the outbreak from May to September 2009. A/H1N1/2009 was detected in 9.1 % (48/526) of samples. One hundred and thirty-seven of the A/H1N1/2009 PCR-negative samples were additionally tested using a RealAccurate Respiratory RT-PCR panel, revealing other respiratory viruses (mainly entero/rhino- and adenoviruses) in 42.3 % (58/137). All methods analysed detected A/H1N1/2009 with excellent specificity but different sensitivities (POC test: 18.2 %; DFA staining: 38.7 %; virus isolation: 45.7 %). Therefore, the POC test was not suitable for diagnosis, detecting A/H1N1/2009 only if present in high concentrations (corresponding median C t value=19.0; range=16.5–21.4). DFA staining was also able to detect A/H1N1/2009 in specimens with a lower virus concentration (median C t value=24.0; range=16.5–29.8). Virus isolation, which was positive after a median time of 7.5 days, was too time-consuming. In summary, DFA staining is superior to POC testing and may be appropriate for patients expected to have a rather high level of virus replication. Nevertheless, in DFA-negative specimens, A/H1N1/2009 should be excluded by RT-PCR.


2000 ◽  
Vol 17 (2) ◽  
pp. 119-126 ◽  
Author(s):  
M Hindiyeh ◽  
C Goulding ◽  
H Morgan ◽  
B Kenyon ◽  
J Langer ◽  
...  

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