The Enigma ML FluAB-RSV assay: a fully automated molecular test for the rapid detection of influenza A, B and respiratory syncytial viruses in respiratory specimens

2014 ◽  
Vol 15 (1) ◽  
pp. 23-32 ◽  
Author(s):  
Simon D Goldenberg ◽  
Jonathan D Edgeworth
Keyword(s):  
Rapid Detection ◽  
Influenza A ◽  
Molecular Test ◽  
Respiratory Syncytial Viruses ◽  
Respiratory Specimens

scholarly journals Comparison of the performance of direct fluorescent antibody staining, a point-of-care rapid antigen test and virus isolation with that of RT-PCR for the detection of novel 2009 influenza A (H1N1) virus in respiratory specimens

2010 ◽  
Vol 59 (6) ◽  
pp. 713-717 ◽  
Author(s):  
Tina Ganzenmueller ◽  
Jeanette Kluba ◽  
Birgit Hilfrich ◽  
Wolfram Puppe ◽  
Willem Verhagen ◽  
...  
Keyword(s):  
Rapid Detection ◽  
Influenza A ◽  
Virus Isolation ◽  
H1n1 Virus ◽  
Point Of Care ◽  
Fluorescent Antibody ◽  
Rt Pcr ◽  
Influenza A H1n1 ◽  
Direct Fluorescent Antibody ◽  
Respiratory Specimens

Although infections with the novel pandemic 2009 influenza A (H1N1) virus (A/H1N1/2009) appeared to be relatively mild during the first summer of circulation (‘off season’), there has been significant morbidity and hospitalization and several fatal cases. Thus, rapid detection of A/H1N1/2009 is crucial for efficient treatment and infection control measures. In contrast to seasonal influenza, where point-of-care (POC) rapid antigen tests and direct fluorescent antibody (DFA) staining ensure rapid detection, diagnosis of A/H1N1/2009 has so far been based on RT-PCR. This study retrospectively compared the performance of the Quidel QuickVue POC test, DFA staining and virus isolation with that of RT-PCR for A/H1N1/2009 detection in 526 respiratory specimens collected during the first wave of the outbreak from May to September 2009. A/H1N1/2009 was detected in 9.1 % (48/526) of samples. One hundred and thirty-seven of the A/H1N1/2009 PCR-negative samples were additionally tested using a RealAccurate Respiratory RT-PCR panel, revealing other respiratory viruses (mainly entero/rhino- and adenoviruses) in 42.3 % (58/137). All methods analysed detected A/H1N1/2009 with excellent specificity but different sensitivities (POC test: 18.2 %; DFA staining: 38.7 %; virus isolation: 45.7 %). Therefore, the POC test was not suitable for diagnosis, detecting A/H1N1/2009 only if present in high concentrations (corresponding median C t value=19.0; range=16.5–21.4). DFA staining was also able to detect A/H1N1/2009 in specimens with a lower virus concentration (median C t value=24.0; range=16.5–29.8). Virus isolation, which was positive after a median time of 7.5 days, was too time-consuming. In summary, DFA staining is superior to POC testing and may be appropriate for patients expected to have a rather high level of virus replication. Nevertheless, in DFA-negative specimens, A/H1N1/2009 should be excluded by RT-PCR.

scholarly journals Performance assessment of Respiratory Viral ELITe MGB® assay for the quantitative detection of influenza A/B and respiratory syncytial viruses

2020 ◽  
Author(s):  
Antonio Piralla ◽  
Federica Giardina ◽  
Alice Fratini ◽  
Davide Sapia ◽  
Francesca Rovida ◽  
...  
Keyword(s):  
Influenza A ◽  
Linear Regression Analysis ◽  
Simultaneous Detection ◽  
Great Majority ◽  
Quantitative Detection ◽  
Total Percentage ◽  
Respiratory Syncytial Viruses ◽  
Syncytial Virus ◽  
Tract Infections ◽  
Respiratory Specimens

AbstractInfluenza (Flu) and respiratory syncytial virus (RSV) are responsible for lower respiratory tract infections (LRTIs) associated with significant hospitalization among young children. In the present study, the performances of a triplex PCR assay detecting Flu A/B and RSV were compared with our in-house single-plex assays using 160 stored respiratory specimens previously tested using a panel of laboratory-developed real-time RT-PCR. Of them, 61 were positive for FluA, 41 for FluB, and 58 for RSV. All samples were retrospectively quantified with Respiratory Viral (RV) ELITe MGB® Panel (ELITechGroup Molecular Diagnostics, Puteaux, France) processed using ELITe InGenius® system. Overall, the total percentage agreement observed was 93.4% (57/61) for FluA, 92.7% (38/41) for FluB, and 86.2% (50/58) for RSV. A significant correlation of VL values was observed between the two methods for FluA and RSV (ρ= 0.91 and 0.84). This finding was supported by the strength of agreement between the two methods, as showed by the linear regression analysis (R2 =0.84 and 0.80). FluB viral load values measured by RV Panel were less significantly correlated (ρ= 0.77 and R2 =0.56). The bland-Altman analysis showed how 84.2% (48/57) of FluA and 86.0% of RSV (43/50) samples fell within ±1.0 Log10 variation from our laboratory results, while only 21.1% (8/38) of FluB results fell within this range. The great majority of FluB samples (29/30) outside range had values higher than +1.0 Log10 (median +2.1 Log10 range +1.0 to +3.5 Log10). In conclusion, RV ELITe MGB® Panel constitutes a valid and robust system for simultaneous detection and quantification of Flu A/B and RSV.

Evaluation of BioStar® FLU OIA® assay for rapid detection of influenza A and B viruses in respiratory specimens

2000 ◽  
Vol 17 (2) ◽  
pp. 119-126 ◽  
Author(s):  
M Hindiyeh ◽  
C Goulding ◽  
H Morgan ◽  
B Kenyon ◽  
J Langer ◽  
...  
Keyword(s):  
Rapid Detection ◽  
Influenza A ◽  
Respiratory Specimens

Development of an antigen-ELISA and a colloidal gold–based immunochromatographic strip based on monoclonal antibodies for detection of avian influenza A(H5) viruses

2021 ◽  
pp. 104063872110275
Author(s):  
Yixin Xiao ◽  
Fan Yang ◽  
Fumin Liu ◽  
Linfang Cheng ◽  
Hangping Yao ◽  
...  
Keyword(s):  
Avian Influenza ◽  
Disease Virus ◽  
Colloidal Gold ◽  
Rapid Detection ◽  
Influenza A ◽  
Field Investigation ◽  
Cross Reactivity ◽  
Detection Methods ◽  
Immunochromatographic Strip ◽  
Antigen Elisa

Avian influenza A(H5) viruses (avian IAVs) pose a major threat to the economy and public health. We developed an antigen-ELISA (ag-ELISA) and a colloidal gold–based immunochromatographic strip for the rapid detection of avian A(H5) viruses. Both detection methods displayed no cross-reactivity with other viruses (e.g., other avian IAVs, infectious bursal disease virus, Newcastle disease virus, infectious bronchitis virus, avian paramyxovirus). The ag-ELISA was sensitive down to 0.5 hemagglutinin (HA) units/100 µL of avian A(H5) viruses and 7.5 ng/mL of purified H5 HA proteins. The immunochromatographic strip was sensitive down to 1 HA unit/100 µL of avian A(H5) viruses. Both detection methods exhibited good reproducibility with CVs < 10%. For 200 random poultry samples, the sensitivity and specificity of the ag-ELISA were 92.6% and 98.8%, respectively, and for test strips were 88.9% and 98.3%, respectively. Both detection methods displayed high specificity, sensitivity, and stability, making them suitable for rapid detection and field investigation of avian A(H5) viruses.

scholarly journals Microfluidic Chip for Molecular Amplification of Influenza A RNA in Human Respiratory Specimens

PLoS ONE ◽  
2012 ◽  
Vol 7 (3) ◽  
pp. e33176 ◽  
Author(s):  
Qingqing Cao ◽  
Madhumita Mahalanabis ◽  
Jessie Chang ◽  
Brendan Carey ◽  
Christopher Hsieh ◽  
...  
Keyword(s):  
Microfluidic Chip ◽  
Influenza A ◽  
Respiratory Specimens

scholarly journals Automated multiplex nucleic acid tests for rapid detection of SARS-CoV-2, influenza A and B infection with direct reverse-transcription quantitative PCR (dirRT-qPCR) assay in a centrifugal microfluidic platform

RSC Advances ◽  
2020 ◽  
Vol 10 (56) ◽  
pp. 34088-34098
Author(s):  
Minghui Ji ◽  
Yun Xia ◽  
Jacky Fong-Chuen Loo ◽  
Lang Li ◽  
Ho-Pui Ho ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Quantitative Pcr ◽  
Reverse Transcription ◽  
Rapid Detection ◽  
Influenza A ◽  
Qpcr Assay ◽  
Multiplex Detection ◽  
Microfluidic Platform ◽  
Reverse Transcription Quantitative Pcr ◽  
Nucleic Acid Tests

Development of a microfluidic disc-direct reverse-transcription quantitative PCR platform to perform automated multiplex nucleic acid tests for rapid multiplex detection of disease infection.

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