scholarly journals Broth Microdilution Susceptibility Testing of Brucella Species: Quality Control Limits for Ten Antimicrobial Agents against Three Standard Quality Control Strains

2005 ◽  
Vol 43 (11) ◽  
pp. 5804-5807 ◽  
Author(s):  
S. D. Brown ◽  
M. M. Traczewski ◽  
Keyword(s):  
Quality Control ◽  
Antimicrobial Agents ◽  
Susceptibility Testing ◽  
Brucella Species ◽  
Broth Microdilution ◽  
Standard Quality ◽  
Control Limits

scholarly journals Provisional Use of CLSI-Approved Quality Control Strains for Antimicrobial Susceptibility Testing of Mycoplasma (‘Mesomycoplasma’) hyorhinis

2021 ◽  
Vol 9 (9) ◽  
pp. 1829
Author(s):  
Lisa Käbisch ◽  
Anne-Kathrin Schink ◽  
Corinna Kehrenberg ◽  
Stefan Schwarz
Keyword(s):  
Quality Control ◽  
Antimicrobial Susceptibility ◽  
Antimicrobial Agents ◽  
Susceptibility Testing ◽  
Antimicrobial Treatment ◽  
Antimicrobial Susceptibility Testing ◽  
Broth Microdilution ◽  
Minimal Inhibitory Concentrations ◽  
Microtiter Plates ◽  
And Staphylococcus Aureus

Antimicrobial susceptibility testing (AST) should be conducted in a standardized manner prior to the start of an antimicrobial treatment. For fastidious bacteria, such as porcine Mycoplasma (‘Mesomycoplasma’) spp., specifically M. hyorhinis, neither guidelines or standards for the performance of AST, nor quality control strains for the validation of AST results are approved by organizations like the Clinical and Laboratory Standards Institute (CLSI) or the European Committee on Antimicrobial Susceptibility Testing (EUCAST). The CLSI- and EUCAST-approved quality control strains Enterococcus faecalis ATCC 29212 and Staphylococcus aureus ATCC 29213 were chosen to validate AST by broth microdilution using modified Friis broth, developed as growth medium for porcine Mycoplasma (‘Mesomycoplasma’) spp. The antimicrobial agents doxycycline, enrofloxacin, erythromycin, florfenicol, gentamicin, marbofloxacin, tetracycline, tiamulin, tilmicosin, tulathromycin, and tylosin were examined using customized SensititreTM microtiter plates. Minimal inhibitory concentrations, determined after 24, 48, and 72 h, were mostly within the CLSI-approved quality control ranges for defined antimicrobial agents. We propose the use of the combination of E. faecalis ATCC 29212 and S. aureus ATCC 29213 as surrogate quality control strains for the validation of future AST results obtained for M. hyorhinis by broth microdilution using modified Friis broth.

scholarly journals Broth Microdilution Susceptibility Testing of Campylobacter jejuni and the Determination of Quality Control Ranges for Fourteen Antimicrobial Agents

2006 ◽  
Vol 44 (2) ◽  
pp. 677-677 ◽  
Author(s):  
P. F. McDermott ◽  
S. M. Bodeis-Jones ◽  
T. R. Fritsche ◽  
R. N. Jones ◽  
R. D. Walker ◽  
...  
Keyword(s):  
Quality Control ◽  
Campylobacter Jejuni ◽  
Antimicrobial Agents ◽  
Susceptibility Testing ◽  
Broth Microdilution

scholarly journals Impact of Variations in Test Method Parameters onIn VitroActivity of Surotomycin against Clostridium difficile and Surotomycin Quality Control Limits for Broth Microdilution and Agar Dilution Susceptibility Testing

2015 ◽  
Vol 54 (3) ◽  
pp. 749-753 ◽  
Author(s):  
Maria M. Traczewski ◽  
Jennifer Deane ◽  
Daniel Sahm ◽  
Steven D. Brown ◽  
Laurent Chesnel
Keyword(s):  
Quality Control ◽  
Clostridium Difficile ◽  
Calcium Concentration ◽  
Susceptibility Testing ◽  
Test Method ◽  
Test Parameter ◽  
Content Type ◽  
Parameter Variations ◽  
Agar Dilution ◽  
Control Limits

Test parameter variations were evaluated for their effects on surotomycin MICs. Calcium concentration was the only variable that influenced MICs; therefore, 50 μg/ml (standard for lipopeptide testing) is recommended. Quality control ranges forClostridium difficile(0.12 to 1 μg/ml) andEggerthella lenta(broth, 1 to 4 μg/ml; agar, 1 to 8 μg/ml) were approved by the Clinical and Laboratory Standards Institute based on these data.

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