scholarly journals The Transmission Efficiency of Tomato Yellow Leaf Curl Virus by the Whitefly Bemisia tabaci Is Correlated with the Presence of a Specific Symbiotic Bacterium Species

2010 ◽  
Vol 84 (18) ◽  
pp. 9310-9317 ◽  
Author(s):  
Yuval Gottlieb ◽  
Einat Zchori-Fein ◽  
Netta Mozes-Daube ◽  
Svetlana Kontsedalov ◽  
Marisa Skaljac ◽  
...  
Keyword(s):  
Bemisia Tabaci ◽  
Tomato Yellow Leaf ◽  
Yellow Leaf ◽  
Groel Protein ◽  
Q Biotype ◽  
Leaf Curl Virus ◽  
B Biotype ◽  
Leaf Curl

ABSTRACT Tomato yellow leaf curl virus (TYLCV) (Geminiviridae: Begomovirus) is exclusively vectored by the whitefly Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae). TYLCV transmission depends upon a 63-kDa GroEL protein produced by the vector's endosymbiotic bacteria. B. tabaci is a species complex comprising several genetically distinct biotypes that show different secondary-symbiont fauna. In Israel, the B biotype harbors Hamiltonella, and the Q biotype harbors Wolbachia and Arsenophonus. Both biotypes harbor Rickettsia and Portiera (the obligatory primary symbionts). The aim of this study was to determine which B. tabaci symbionts are involved in TYLCV transmission using B. tabaci populations collected in Israel. Virus transmission assays by B. tabaci showed that the B biotype efficiently transmits the virus, while the Q biotype scarcely transmits it. Yeast two-hybrid and protein pulldown assays showed that while the GroEL protein produced by Hamiltonella interacts with TYLCV coat protein, GroEL produced by Rickettsia and Portiera does not. To assess the role of Wolbachia and Arsenophonus GroEL proteins (GroELs), we used an immune capture PCR (IC-PCR) assay, employing in vivo- and in vitro-synthesized GroEL proteins from all symbionts and whitefly artificial feeding through membranes. Interaction between GroEL and TYLCV was found to occur in the B biotype, but not in the Q biotype. This assay further showed that release of virions protected by GroEL occurs adjacent to the primary salivary glands. Taken together, the GroEL protein produced by Hamiltonella (present in the B biotype, but absent in the Q biotype) facilitates TYLCV transmission. The other symbionts from both biotypes do not seem to be involved in transmission of this virus.

Control of the Bemisia tabaci/Tomato yellow leaf curl virus complex on protected tomato crops in Algarve (Portugal)*

EPPO Bulletin ◽  
2002 ◽  
Vol 32 (1) ◽  
pp. 31-35
Author(s):  
A. F. Arsenio ◽  
E. Neto ◽  
N. Ramos ◽  
S. Mangerico ◽  
E. Fortunato ◽  
...  
Keyword(s):  
Bemisia Tabaci ◽  
Tomato Yellow Leaf ◽  
Yellow Leaf ◽  
Virus Complex ◽  
Leaf Curl Virus ◽  
Leaf Curl

scholarly journals Rapid accumulation and low degradation: key parameters of Tomato yellow leaf curl virus persistence in its insect vector Bemisia tabaci

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Nathalie Becker ◽  
Loup Rimbaud ◽  
Frédéric Chiroleu ◽  
Bernard Reynaud ◽  
Gaël Thébaud ◽  
...  
Keyword(s):  
Bemisia Tabaci ◽  
Tomato Yellow Leaf ◽  
Insect Vector ◽  
Yellow Leaf ◽  
Virus Persistence ◽  
Rapid Accumulation ◽  
Leaf Curl Virus ◽  
Leaf Curl

scholarly journals The Influence of Visual and Olfactory Cues in Host Selection for Bemisia tabaci Biotype B in the Presence or Absence of Tomato Yellow Leaf Curl Virus

Insects ◽  
2020 ◽  
Vol 11 (2) ◽  
pp. 115 ◽  
Author(s):  
Nicholas Johnston ◽  
Xavier Martini
Keyword(s):  
Bemisia Tabaci ◽  
Host Selection ◽  
Tomato Yellow Leaf ◽  
Olfactory Cues ◽  
Yellow Leaf ◽  
Agricultural Pests ◽  
Sensory Stimuli ◽  
Infected Tomato ◽  
Leaf Curl Virus ◽  
Leaf Curl

The silverleaf whitefly, Bemisia tabaci, is one of the most destructive agricultural pests in the world, vectoring a large number of devastating viruses, including Tomato Yellow Leaf Curl Virus (TYLCV). When selecting a host, B. tabaci is primarily influenced by a range of visual and olfactory cues. Therefore, elucidating how such cues become modified in the presence of whitefly-vectored begomoviruses is critical to better understanding the epidemiology of many economically important diseases. The goal of this study was to determine how both visual and odor cues interact in the presence of TYLCV. In Y-tube olfactometer assays, whiteflies were submitted to a range of isolated visual and olfactory cues to determine behavioral changes. B. tabaci choices were then compared to both stimuli combined in the presence or absence of TYLCV. Under visual stimuli only, B. tabaci exhibited a visual attraction to the color yellow, TYLCV-infected tomato leaves, and TYLCV-infected tomato volatiles. Attraction was the strongest overall when both visual and olfactory cues from TYLCV-symptomatic tomato plants were combined, as opposed to a single isolated cue. These results highlight the importance of both sensory stimuli during B. tabaci host selection in the presence of an associated begomovirus.

scholarly journals Bio-rational management of whitefly (Bemisia tabaci) for suppressing tomato yellow leaf curl virus

2016 ◽  
Vol 41 (4) ◽  
pp. 583-597
Author(s):  
MM Alam ◽  
MN Islam ◽  
MZ Haque ◽  
R Humayun ◽  
KM Khalequzzaman
Keyword(s):  
Bemisia Tabaci ◽  
Untreated Control ◽  
Agricultural Research ◽  
Tomato Yellow Leaf ◽  
Diseased Plant ◽  
Strong Positive Correlation ◽  
Yellow Leaf ◽  
Rational Management ◽  
Leaf Curl Virus ◽  
Leaf Curl

Whitefly (Bemisia tabaci) is the vector of tomato yellow leaf curl virus (TYLCV), is a serious pest of vegetables and other crops worldwide. The experiment was conducted at Bangladesh Agricultural Research institute (BARI) during two consecutive years of 2009 and 2010 to select a suitable bio-rational management practice against white fly, transmitting TYLCV. Treatments comprising tomato variety Ratan with diseased plant uprooting, spraying admire, applying admire on trap crop (marigold), spraying neem, sesame and mustard oil with trix and the untreated control were used in this experiment. The variety Opurba with similar materials as described above was used. Percent virus infected tomato plants ranged from 1.33 to 19.00 in two consecutive years, where the highest infection was recorded in control plot with variety Opurba and the lowest was recorded in variety Ratan treated with Admire. Consequently, the highest yield (47.70 and 52.36 t ha-1 in 1st and 2nd year, respectively) in the plots of variety Ratan treated with admire and the lowest yield was recorded in untreated control plots with variety Opurba (14.75 and 30.30 t ha-1) for the two consecutive years. A strong positive correlation was observed between whitefly population and % TYLCV infection for both the years and both varieties of tomato. While a negative correlation was observed between % TYLCV infection with number of fruits plants-1 and yield (t ha-1) for both the years and in both varieties of tomato. These results are consistent with the occurrences of TYLCV, which have been associated with the percent virus infection in relation to yield and yield contributing characters of tomato.Bangladesh J. Agril. Res. 41(4): 583-597, December 2016

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