scholarly journals Association of Rice Gall Dwarf Virus with Microtubules Is Necessary for Viral Release from Cultured Insect Vector Cells

2009 ◽  
Vol 83 (20) ◽  
pp. 10830-10835 ◽  
Author(s):  
Taiyun Wei ◽  
Tamaki Uehara-Ichiki ◽  
Naoyuki Miyazaki ◽  
Hiroyuki Hibino ◽  
Kenji Iwasaki ◽  
...  
Keyword(s):  
Insect Cells ◽  
Core Protein ◽  
Dwarf Virus ◽  
Insect Vector ◽  
Viral Transport ◽  
Transmission Electron ◽  
Viral Particles ◽  
The Family ◽  
Rice Gall Dwarf Virus ◽  
Vector Insect

ABSTRACT Vector insect cells infected with Rice gall dwarf virus, a member of the family Reoviridae, contained the virus-associated microtubules adjacent to the viroplasms, as revealed by transmission electron, electron tomographic, and confocal microscopy. The viroplasms, putative sites of viral replication, contained the nonstructural viral proteins Pns7 and Pns12, as well as core protein P5, of the virus. Microtubule-depolymerizing drugs suppressed the association of viral particles with microtubules and prevented the release of viruses from cells without significantly affecting viral multiplication. Thus, microtubules appear to mediate viral transport within and release of viruses from infected vector cells.

scholarly journals Rice dwarf virus is engulfed into and released via vesicular compartments in cultured insect vector cells

2008 ◽  
Vol 89 (11) ◽  
pp. 2915-2920 ◽  
Author(s):  
Taiyun Wei ◽  
Hiroyuki Hibino ◽  
Toshihiro Omura
Keyword(s):  
Brefeldin A ◽  
Virus Multiplication ◽  
Dwarf Virus ◽  
Insect Vector ◽  
Rice Dwarf Virus ◽  
Transmission Electron ◽  
Viral Particles ◽  
Infected Cells ◽  
Vector Insect ◽  
Butanedione Monoxime

Vector insect cells infected with Rice dwarf virus had vesicular compartments containing viral particles located adjacent to the viroplasm when examined by transmission electron and confocal microscopy. Such compartments were often at the periphery of infected cells. Inhibitors of vesicular transport, brefeldin A and monensin, and an inhibitor of myosin motor activity, butanedione monoxime, abolished the formation of such vesicles and prevented the release of viral particles from infected cells without significant effects on virus multiplication. Furthermore, the actin-depolymerizing drug, cytochalasin D, inhibited the formation of actin filaments without significantly interfering with formation of vesicular compartments and the release of viruses from treated cells. These results together revealed intracellular vesicular compartments as a mode for viral transport in and release from insect vector cells infected with a plant-infecting reovirus.

scholarly journals Expression of rice gall dwarf virus outer coat protein gene (S8) in insect cells

2010 ◽  
Vol 25 (6) ◽  
pp. 401-408 ◽  
Author(s):  
Guo-cheng Fan ◽  
Fang-luan Gao ◽  
Tai-yun Wei ◽  
Mei-ying Huang ◽  
Li-yan Xie ◽  
...  
Keyword(s):  
Coat Protein ◽  
Coat Protein Gene ◽  
Insect Cells ◽  
Protein Gene ◽  
Dwarf Virus ◽  
Outer Coat ◽  
Rice Gall Dwarf Virus

scholarly journals Adverse Effects of Rice gall dwarf virus upon its Insect Vector Recilia dorsalis (Hemiptera: Cicadellidae)

Plant Disease ◽  
2016 ◽  
Vol 100 (4) ◽  
pp. 784-790 ◽  
Author(s):  
Yong Chen ◽  
Chengcong Lu ◽  
Manman Li ◽  
Wei Wu ◽  
Guohui Zhou ◽  
...  
Keyword(s):  
Adverse Effects ◽  
Molecular Level ◽  
Southern China ◽  
Culture Method ◽  
Protein Product ◽  
Dwarf Virus ◽  
Insect Vector ◽  
Direct Effects ◽  
Rice Gall Dwarf Virus ◽  
Polymerase Chain

Rice gall dwarf virus (RGDV), a plant reovirus that threatens rice production in Southeast Asia and Southern China, is transmitted by the leafhopper vector Recilia dorsalis in a persistent-propagative manner. To assess the direct effects of RGDV on R. dorsalis, we established an infected leafhopper population from eggs laid by viruliferous females using the water-soaked filter paper culture method. Life history parameters indicated that the virus was harmful to its vector in terms of all biotic indices, including reduced survival rate, emergence rate, fecundity, and longevity of adults, compared with a nonviruliferous control population. Those findings were supported by systematic monitoring of viruliferous rates of R. dorsalis in different overwintering generations. To better elucidate the adverse effects of RGDV on its vector, we measured fecundity at the molecular level using quantitative reverse-transcription polymerase chain reaction and Western blot assays, which revealed differential expression of vitellogenin (Vg) in viruliferous versus nonviruliferous adult females. We infer that RGDV reduced levels of Vg transcript and protein product, resulting in the lower fecundity of its vector. Overall, this study demonstrates how RGDV exerts an adverse effect on R. dorsalis, which hinders the expansion of viruliferous populations of the insect.

scholarly journals Particles of Rice Gall Dwarf Virus in Thin Sections of Diseased Rice Plants and Insect Vector Cells

1985 ◽  
Vol 66 (12) ◽  
pp. 2581-2587 ◽  
Author(s):  
T. Omura ◽  
H. Hibino ◽  
H. Inoue ◽  
T. Tsuchizaki
Keyword(s):  
Dwarf Virus ◽  
Insect Vector ◽  
Thin Sections ◽  
Rice Plants ◽  
Rice Gall Dwarf Virus

scholarly journals Synergism Between Southern rice black-streaked dwarf virus and Rice ragged stunt virus Enhances Their Insect Vector Acquisition

2014 ◽  
Vol 104 (7) ◽  
pp. 794-799 ◽  
Author(s):  
Shu Li ◽  
Han Wang ◽  
Guohui Zhou
Keyword(s):  
Brown Planthopper ◽  
Nilaparvata Lugens ◽  
Plant Viruses ◽  
Dwarf Virus ◽  
Insect Vector ◽  
Sogatella Furcifera ◽  
Rice Plants ◽  
The Family ◽  
Low Incidence ◽  
Rice Ragged Stunt Virus

Southern rice black-streaked dwarf virus (SRBSDV), a tentative species in the genus Fijivirus, family Reoviridae, is a novel rice virus transmitted by the white-backed planthopper (Sogatella furcifera). Since its discovery in 2001, SRBSDV has spread rapidly throughout eastern and southeastern Asia and caused large rice losses in China and Vietnam. Rice ragged stunt virus (RRSV) (genus Oryzavirus, family Reoviridae) is a common rice virus vectored by the brown planthopper (Nilaparvata lugens). RRSV is also widely distributed in eastern and southeastern Asia but has not previously caused serious problems in China owing to its low incidence. With SRBSDV's spread, however, RRSV has become increasingly common in China, and is frequently found in co-infection with SRBSDV. In this study, we show that SRBSDV and RRSV interact synergistically, the first example of synergism between plant viruses in the family Reoviridae. Rice plants co-infected with both viruses displayed enhanced stunting, earlier symptoms, and higher virus titers compared with singly infected plants. Furthermore, white-backed and brown planthoppers acquired SRBSDV and RRSV, respectively, from co-infected plants at higher rates. We propose that increased RRSV incidence in Chinese fields is partly due to synergism between SRBSDV and RRSV.

Three-Dimensional Analysis of the Association of Viral Particles with Mitochondria during the Replication of Rice Gall Dwarf Virus

2011 ◽  
Vol 410 (3) ◽  
pp. 436-446 ◽  
Author(s):  
Taiyun Wei ◽  
Naoyuki Miyazaki ◽  
Tamaki Uehara-Ichiki ◽  
Hiroyuki Hibino ◽  
Takumi Shimizu ◽  
...  
Keyword(s):  
Dimensional Analysis ◽  
Three Dimensional ◽  
Dwarf Virus ◽  
Viral Particles ◽  
Rice Gall Dwarf Virus

scholarly journals Sequence Analysis and GTP-Binding Ability of the Minor Core Protein P5 of Rice Gall Dwarf Virus

2002 ◽  
Vol 36 (2) ◽  
pp. 83-87 ◽  
Author(s):  
Kenzaburo ICHIMI ◽  
Akira KIKUCHI ◽  
Yusuke MORIYASU ◽  
Boxiong ZHONG ◽  
Kyoji HAGIWARA ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Core Protein ◽  
Dwarf Virus ◽  
Binding Ability ◽  
Gtp Binding ◽  
Rice Gall Dwarf Virus

scholarly journals Genome Organizations and Functional Analyses of a Novel Gammapartitivirus from Rhizoctonia solani AG-1 IA Strain D122

Viruses ◽  
2021 ◽  
Vol 13 (11) ◽  
pp. 2254
Author(s):  
Meiling Zhang ◽  
Zhenrui He ◽  
Xiaotong Huang ◽  
Canwei Shu ◽  
Erxun Zhou
Keyword(s):  
Rhizoctonia Solani ◽  
Phylogenetic Analyses ◽  
Protoplast Regeneration ◽  
Sequence Alignments ◽  
Reading Frame ◽  
Transmission Electron ◽  
Viral Particles ◽  
The Family ◽  
20 Nm ◽  
Functional Analyses

Here, we describe a novel double-stranded (ds) RNA mycovirus designated Rhizoctonia solani dsRNA virus 5 (RsRV5) from strain D122 of Rhizoctonia solani AG-1 IA, the causal agent of rice sheath blight. The RsRV5 genome consists of two segments of dsRNA (dsRNA-1, 1894 bp and dsRNA-2, 1755 bp), each possessing a single open reading frame (ORF). Sequence alignments and phylogenetic analyses showed that RsRV5 is a new member of the genus Gammapartitivirus in the family Partitiviridae. Transmission electron microscope (TEM) images revealed that RsRV5 has isometric viral particles with a diameter of approximately 20 nm. The mycovirus RsRV5 was successfully removed from strain D122 by using the protoplast regeneration technique, thus resulting in derivative isogenic RsRV5-cured strain D122-P being obtained. RsRV5-cured strain D122-P possessed the traits of accelerated mycelial growth rate, increased sclerotia production and enhanced pathogenicity to rice leaves compared with wild type RsRV5-infection strain D122. Transcriptome analysis showed that three genes were differentially expressed between two isogenic strains, D122 and D122-P. These findings provided new insights into the molecular mechanism of the interaction between RsRV5 and its host, D122 of R. solani AG-1 IA.

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