Bovine Nebovirus Interacts with a Wide Spectrum of Histo-Blood Group Antigens

ABSTRACTSome viruses within theCaliciviridaefamily initiate their replication cycle by attachment to cell surface carbohydrate moieties, histo-blood group antigens (HBGAs), and/or terminal sialic acids (SAs). Although bovine nebovirus (BNeV), one of the enteric caliciviruses, is an important causative agent of acute gastroenteritis in cattle, its attachment factors and possibly other cellular receptors remain unknown. Using a comprehensive series of protein-ligand biochemical assays, we sought to determine whether BNeV recognizes cell surface HBGAs and/or SAs as attachment factors. It was found that BNeV virus-like particles (VLPs) bound to A type/H type 2/LeyHBGAs expressed in the bovine digestive tract and are related to HBGAs expressed in humans and other host species, suggesting a wide spectrum of HBGA recognition by BNeV. BNeV VLPs also bound to a large variety of different bovine and human saliva samples of all ABH and Lewis types, supporting previously obtained results and suggesting a zoonotic potential of BNeV transmission. Removal of α1,2-linked fucose and α1,3/4-linked fucose epitopes of target HBGAs by confirmation-specific enzymes reduced the binding of BNeV VLPs to synthetic HBGAs, bovine and human saliva, cultured cell lines, and bovine small intestine mucosa, further supporting a wide HBGA binding spectrum of BNeV through recognition of α1,2-linked fucose and α1,3/4-linked fucose epitopes of targeted HBGAs. However, removal of terminal α2,3- and α2,6-linked SAs by their specific enzyme had no inhibitory effects on binding of BNeV VLPs, indicating that BNeV does not use terminal SAs as attachment factors. Further details of the binding specificity of BNeV remain to be explored.IMPORTANCEEnteric caliciviruses such as noroviruses, sapoviruses, and recoviruses are the most important etiological agents of severe acute gastroenteritis in humans and many other mammalian host species. They initiate infection by attachment to cell surface carbohydrate moieties, HBGAs, and/or terminal SAs. However, the attachment factor(s) for BNeV, a recently classified enteric calicivirus genus/type species, remains unexplored. Here, we demonstrate that BNeV VLPs have a wide spectrum of binding to synthetic HBGAs, bovine and human saliva samples, and bovine duodenal sections. We further discovered that α1,2-linked fucose and α1,3/4-linked fucose epitopes are essential for binding of BNeV VLPs. However, BNeV VLPs do not bind to terminal SAs on cell carbohydrates. Continued investigation regarding the proteinaceous receptor(s) will be necessary for better understanding of the tropism, pathogenesis, and host range of this important viral genus.

Download Full-text

Norovirus and Histo-Blood Group Antigens: Demonstration of a Wide Spectrum of Strain Specificities and Classification of Two Major Binding Groups among Multiple Binding Patterns

Journal of Virology ◽

10.1128/jvi.79.11.6714-6722.2005 ◽

2005 ◽

Vol 79 (11) ◽

pp. 6714-6722 ◽

Cited By ~ 278

Author(s):

Pengwei Huang ◽

Tibor Farkas ◽

Weiming Zhong ◽

Ming Tan ◽

Scott Thornton ◽

...

Keyword(s):

Ligand Binding ◽

Blood Group ◽

Acute Gastroenteritis ◽

Specific Binding ◽

Wide Spectrum ◽

Phylogenetic Analyses ◽

Lewis Antigens ◽

Blood Group Antigens ◽

Multiple Binding

ABSTRACT Noroviruses, an important cause of acute gastroenteritis, have been found to recognize human histo-blood group antigens (HBGAs) as receptors. Four strain-specific binding patterns to HBGAs have been described in our previous report. In this study, we have extended the binding patterns to seven based on 14 noroviruses examined. The oligosaccharide-based assays revealed additional epitopes that were not detected by the saliva-based assays. The seven patterns have been classified into two groups according to their interactions with three major epitopes (A/B, H, and Lewis) of human HBGAs: the A/B-binding group and the Lewis-binding group. Strains in the A/B binding group recognize the A and/or B and H antigens, but not the Lewis antigens, while strains in the Lewis-binding group react only to the Lewis and/or H antigens. This classification also resulted in a model of the norovirus/HBGA interaction. Phylogenetic analyses showed that strains with identical or closely related binding patterns tend to be clustered, but strains in both binding group can be found in both genogroups I and II. Our results suggest that noroviruses have a wide spectrum of host range and that human HBGAs play an important role in norovirus evolution. The high polymorphism of the human HBGA system, the involvement of multiple epitopes, and the typical protein/carbohydrate interaction between norovirus VLPs and HBGAs provide an explanation for the virus-ligand binding diversities.

Download Full-text

Interaction ofVicia gramineaAnti-N Lectin with Cell Surface Glycoproteins from Erythrocytes with Rare Blood Group Antigens

Hoppe-Seyler´s Zeitschrift für physiologische Chemie ◽

10.1515/bchm2.1984.365.1.469 ◽

1984 ◽

Vol 365 (1) ◽

pp. 469-478 ◽

Cited By ~ 15

Author(s):

Dominique BLANCHARD ◽

Alain ASSERAF ◽

Marie José PRiGENT ◽

John J. MOULDS ◽

Dasnayanee CHANDANAYINGYONG ◽

...

Keyword(s):

Cell Surface ◽

Blood Group ◽

Blood Group Antigens ◽

Cell Surface Glycoproteins ◽

Surface Glycoproteins

Download Full-text

Cell surface glyceraldehyde-3-phosphate dehydrogenase (GAPDH) of Lactobacillus plantarum LA 318 recognizes human A and B blood group antigens

Research in Microbiology ◽

10.1016/j.resmic.2008.07.005 ◽

2008 ◽

Vol 159 (9-10) ◽

pp. 685-691 ◽

Cited By ~ 48

Author(s):

Hideki Kinoshita ◽

Nozomi Wakahara ◽

Masamichi Watanabe ◽

Tomomi Kawasaki ◽

Hiroki Matsuo ◽

...

Keyword(s):

Cell Surface ◽

Lactobacillus Plantarum ◽

Blood Group ◽

Blood Group Antigens ◽

B Blood Group

Download Full-text

Association between circulating rotavirus genotypes and histo‐blood group antigens in the children hospitalized with acute gastroenteritis in Iran

Journal of Medical Virology ◽

10.1002/jmv.26808 ◽

2021 ◽

Author(s):

Mohammad Farahmand ◽

Somayeh Jalilvand ◽

Arash Arashkia ◽

Shohreh Shahmahmoodi ◽

Atefeh Afchangi ◽

...

Keyword(s):

Blood Group ◽

Acute Gastroenteritis ◽

Blood Group Antigens

Download Full-text

The cell surface carbohydrate blood group A regulates the selective fasciculation of regenerating accessory olfactory axons

Brain Research ◽

10.1016/j.brainres.2008.01.084 ◽

2008 ◽

Vol 1203 ◽

pp. 32-38 ◽

Cited By ~ 7

Author(s):

Fatemeh Chehrehasa ◽

Brian Key ◽

James A. St John

Keyword(s):

Cell Surface ◽

Blood Group ◽

Blood Group A ◽

Cell Surface Carbohydrate ◽

Group A

Download Full-text

An examination of co-infection in acute gastroenteritis and histo-blood group antigens leading to viral infection susceptibility

Biomedical Reports ◽

10.3892/br.2016.585 ◽

2016 ◽

Vol 4 (3) ◽

pp. 331-334 ◽

Cited By ~ 2

Author(s):

KENTA FURUYA ◽

HITOSHI NAKAJIMA ◽

YOUSUKE SASAKI ◽

YOSHIHISA URITA

Keyword(s):

Viral Infection ◽

Blood Group ◽

Acute Gastroenteritis ◽

Blood Group Antigens ◽

Infection Susceptibility

Download Full-text

ABO Blood-group Antigens in Oral Cancer

Journal of Dental Research ◽

10.1177/154405910508400103 ◽

2005 ◽

Vol 84 (1) ◽

pp. 21-28 ◽

Cited By ~ 46

Author(s):

E. Dabelsteen ◽

S. Gao

Keyword(s):

Cell Surface ◽

Blood Group ◽

Tumor Development ◽

Gene Promoter ◽

Surface Proteins ◽

Transferase Activity ◽

Blood Group Antigens ◽

Aberrant Expression ◽

Specific Tumor ◽

Altered Blood

Tumor progression is often associated with altered glycosylation of the cell-surface proteins and lipids. The peripheral part of these cell-surface glycoconjugates often carries carbohydrate structures related to the ABO and Lewis blood-group antigens. The expression of histo-blood-group antigens in normal human tissues is dependent on the type of differentiation of the epithelium. In most human carcinomas, including oral carcinoma, a significant event is decreased expression of histo-blood-group antigens A and B. The mechanisms of aberrant expression of blood-group antigens are not clear in all cases. A relative down-regulation of the glycosyltransferase that is involved in the biosynthesis of A and B antigens is seen in oral carcinomas in association with tumor development. The events leading to loss of A transferase activity are related, in some instances, to loss of heterozygosity (LOH) involving chromosome 9q34, which is the locus for the ABO gene, and in other cases, to a hypermethylation of the ABO gene promoter. The fact that hypermethylation targets the ABO locus, but not surrounding genes, suggests that the hypermethylation is a specific tumor-related event. However, since not all situations with lack of expression of A/B antigens can be explained by LOH or hypermethylation, other regulatory factors outside the ABO promoter may be functional in transcriptional regulation of the ABO gene. Altered blood group antigens in malignant oral tissues may indicate increased cell migration. This hypothesis is supported by studies showing that normal migrating oral epithelial cells like malignant cells show lack of expression of A/B antigens, and by studies that target ABH antigens to key receptors controlling adhesion and motility, such as integrins, cadherins, and CD-44.

Download Full-text

Reexpression of blood group ABH antigens on the surface of human thyroid cells in culture.

The Journal of Cell Biology ◽

10.1083/jcb.94.1.193 ◽

1982 ◽

Vol 94 (1) ◽

pp. 193-200 ◽

Cited By ~ 5

Author(s):

E L Khoury

Keyword(s):

Cell Surface ◽

Blood Group ◽

Enzymatic Digestion ◽

Cell Suspensions ◽

Human Thyroid ◽

Blood Group Antigens ◽

Thyroid Cells ◽

Blood Group Abh

Using indirect immunofluorescence (IFL) on viable human thyroid cultures, it has been shown that, although adult follicular cells do not express blood group ABH antigens in vivo, they invariably reexpress the corresponding antigens on the cell surface when cultured in monolayers, even for very short periods. The absence of blood group antigens on noncultured thyroid cells was confirmed by negative IFL on cell suspensions obtained after enzymatic digestion of the glands, whereas these antigens were readily demonstrable on cell suspensions obtained by trypsinization of established monolayers. The quantitative expression of ABH antigens on individual thyroid cells was variable and the cell-surface IFL pattern due to binding of blood group isoantibodies was different from that given by organ-specific thyroid autoantibodies on viable cultures. Reexpression of blood group antigens by cultured thyroid cells could not be related to the secretor status of the donors, the presence of a particular source of serum in the culture medium or cell division in vitro. After 2-3 wk in culture, thyroid cells became morphologically dedifferentiated and no longer displayed blood group antigens, though they still expressed cell-surface beta 2-microglobulin. Fibroblasts present in the primary thyroid cultures were invariably negative for ABH antigens. These results demonstrate that the surface antigenic repertoire of cultured human cells is not necessarily identical to that present on the same cells in vivo. Furthermore, the possibility that blood group natural isoantibodies bind to the cell surface must be taken into account in experiments in which cultured thyroid cells are exposed to human sera.

Download Full-text