scholarly journals Portal-Large Terminase Interactions of the Bacteriophage T4 DNA Packaging Machine Implicate a Molecular Lever Mechanism for Coupling ATPase to DNA Translocation

2012 ◽  
Vol 86 (8) ◽  
pp. 4046-4057 ◽  
Author(s):  
Shylaja Hegde ◽  
Victor Padilla-Sanchez ◽  
Bonnie Draper ◽  
Venigalla B. Rao
Keyword(s):  
Bacteriophage T4 ◽  
Dna Packaging ◽  
Dna Translocation ◽  
Packaging Machine ◽  
Lever Mechanism ◽  
Large Terminase

scholarly journals A Promiscuous DNA Packaging Machine from Bacteriophage T4

PLoS Biology ◽  
2011 ◽  
Vol 9 (2) ◽  
pp. e1000592 ◽  
Author(s):  
Zhihong Zhang ◽  
Vishal I. Kottadiel ◽  
Reza Vafabakhsh ◽  
Li Dai ◽  
Yann R. Chemla ◽  
...  
Keyword(s):  
Bacteriophage T4 ◽  
Dna Packaging ◽  
Promiscuous Dna ◽  
Packaging Machine

scholarly journals In vitro and in vivo delivery of genes and proteins using the bacteriophage T4 DNA packaging machine

2013 ◽  
Vol 110 (15) ◽  
pp. 5846-5851 ◽  
Author(s):  
P. Tao ◽  
M. Mahalingam ◽  
B. S. Marasa ◽  
Z. Zhang ◽  
A. K. Chopra ◽  
...  
Keyword(s):  
Bacteriophage T4 ◽  
Dna Packaging ◽  
Packaging Machine ◽  
In Vivo Delivery

scholarly journals A viral genome packaging ring-ATPase is a flexibly coordinated pentamer

2021 ◽  
Author(s):  
Li Dai ◽  
Digvijay Singh ◽  
Suoang Lu ◽  
Vishal Kottadiel ◽  
Reza Vafabakhsh ◽  
...  
Keyword(s):  
Single Molecule ◽  
Viral Genome ◽  
Motor Coordination ◽  
Bacteriophage T4 ◽  
Dna Packaging ◽  
Biological Functions ◽  
Dna Translocation ◽  
Genome Packaging ◽  
Dna Packaging Motor ◽  
Direct Counting

Multi-subunit ring-ATPases carry out a myriad of biological functions, including genome packaging in viruses. Though the basic structures and functions of these motors have been well-established, the mechanisms of ATPase firing and motor coordination are poorly understood. Here, by direct counting using single-molecule fluorescence, we have determined that the active bacteriophage T4 DNA packaging motor consists of five subunits of gp17. By systematically doping motors with an ATPase-defective subunit and selecting single motors containing a precise count of active/inactive subunit(s), we found, unexpectedly, that the packaging motor can tolerate an inactive sub-unit. However, motors containing an inactive subunit(s) exhibit fewer DNA engagements, a higher failure rate in encapsidation, reduced packaging velocity, and increased pausing. These findings suggest a new packaging model in which the motor, by re-adjusting its grip on DNA, can skip an inactive subunit and resume DNA translocation, contrary to the prevailing notion of strict coordination amongst motor subunits of other packaging motors.

scholarly journals A viral genome packaging ring-ATPase is a flexibly coordinated pentamer

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Li Dai ◽  
Digvijay Singh ◽  
Suoang Lu ◽  
Vishal I. Kottadiel ◽  
Reza Vafabakhsh ◽  
...  
Keyword(s):  
Single Molecule ◽  
Viral Genome ◽  
Motor Coordination ◽  
Bacteriophage T4 ◽  
Dna Packaging ◽  
Biological Functions ◽  
Dna Translocation ◽  
Single Molecule Fluorescence ◽  
Genome Packaging ◽  
Dna Packaging Motor

AbstractMulti-subunit ring-ATPases carry out a myriad of biological functions, including genome packaging in viruses. Though the basic structures and functions of these motors have been well-established, the mechanisms of ATPase firing and motor coordination are poorly understood. Here, using single-molecule fluorescence, we determine that the active bacteriophage T4 DNA packaging motor consists of five subunits of gp17. By systematically doping motors with an ATPase-defective subunit and selecting single motors containing a precise number of active or inactive subunits, we find that the packaging motor can tolerate an inactive subunit. However, motors containing one or more inactive subunits exhibit fewer DNA engagements, a higher failure rate in encapsidation, reduced packaging velocity, and increased pausing. These findings suggest a DNA packaging model in which the motor, by re-adjusting its grip on DNA, can skip an inactive subunit and resume DNA translocation, suggesting that strict coordination amongst motor subunits of packaging motors is not crucial for function.

scholarly journals Single-molecule packaging initiation in real time by a viral DNA packaging machine from bacteriophage T4

2014 ◽  
Vol 111 (42) ◽  
pp. 15096-15101 ◽  
Author(s):  
Reza Vafabakhsh ◽  
Kiran Kondabagil ◽  
Tyler Earnest ◽  
Kyung Suk Lee ◽  
Zhihong Zhang ◽  
...  
Keyword(s):  
Real Time ◽  
Single Molecule ◽  
Bacteriophage T4 ◽  
Dna Packaging ◽  
Viral Dna ◽  
Packaging Machine ◽  
Viral Dna Packaging

scholarly journals The C-terminal domain of the bacteriophage T4 terminase docks on the prohead portal clip region during DNA packaging

Virology ◽  
2013 ◽  
Vol 446 (1-2) ◽  
pp. 293-302 ◽  
Author(s):  
Aparna Banerjee Dixit ◽  
Krishanu Ray ◽  
Julie A. Thomas ◽  
Lindsay W. Black
Keyword(s):  
Bacteriophage T4 ◽  
Dna Packaging ◽  
Terminal Domain
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