scholarly journals Effect of Actinomycin D on Virus-induced Ribonucleic Acid Polymerase Formation in Foot-and-Mouth Disease Virus-Infected Baby Hamster Kidney Cells

1967 ◽  
Vol 1 (6) ◽  
pp. 1130-1134 ◽  
Author(s):  
Jerome Polatnick ◽  
Ralph B. Arlinghaus
Keyword(s):  
Disease Virus ◽  
Ribonucleic Acid ◽  
Actinomycin D ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Kidney Cells ◽  
Baby Hamster Kidney ◽  
Mouth Disease Virus ◽  
Foot And Mouth ◽  
Baby Hamster Kidney Cells

scholarly journals A study of the heterogeneous 37s ribonucleic acid induced by foot-and-mouth-disease virus

1968 ◽  
Vol 107 (3) ◽  
pp. 395-401 ◽  
Author(s):  
T. F. Wild ◽  
S J Martin ◽  
F. Brown
Keyword(s):  
Disease Virus ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Kidney Cells ◽  
Virus Particles ◽  
Mouth Disease Virus ◽  
Heterogeneous Nature ◽  
Foot And Mouth ◽  
Sepharose 4B ◽  
Baby Hamster Kidney Cells

1. The 37s RNA induced in baby-hamster kidney cells by infection with foot-and-mouth-disease virus was examined on sucrose gradients and by filtration through Sepharose 4B. 2. The RNA sedimented faster (37s) and as a broader band than the 35s RNA from purified virus. 3. Treatment with deoxyribonuclease, Pronase or amylase did not alter the sedimentation profile of the 37s RNA. 4. Treatment of individual fractions of the RNA with phenol, dimethyl sulphoxide or methylCellosolve did not decrease the sedimentation rate of the faster-sedimenting molecules. 5. Sedimentation in sucrose gradients of different ionic strengths or containing EDTA had no effect on the heterogeneous nature of the profile. 6. On filtration through Sepharose 4B columns, the 37s virus-induced RNA was eluted before viral RNA. 7. Only 20% of the rapidly sedimenting RNA was incorporated into complete virus particles.

scholarly journals Effect of actinomycin D and guanidine on the formation of a ribonucleic acid polymerase induced by foot-and-mouth-disease virus and on the replication of virus and viral ribonucleic acid

1969 ◽  
Vol 112 (3) ◽  
pp. 317-323 ◽  
Author(s):  
D. N. Black ◽  
F. Brown
Keyword(s):  
Rna Polymerase ◽  
Disease Virus ◽  
Ribonucleic Acid ◽  
Actinomycin D ◽  
Foot And Mouth Disease ◽  
Growth Cycle ◽  
Mouth Disease ◽  
Viral Rna ◽  
Mouth Disease Virus ◽  
Foot And Mouth

The RNA-dependent RNA polymerase induced in baby-hamster kidney cells by infection with foot-and-mouth-disease virus can be detected as early as 60min. after infection, which is 60min. before viral RNA synthesis commences. The time at which the polymerase can first be detected coincides with the latest time at which actinomycin D (50μg./107 cells) or guanidine (1mg./107 cells) inhibits virus replication. However, by increasing the concentration of guanidine, viral replication can be inhibited later in the growth cycle, casting doubt on the validity of the hypothesis that guanidine acts specifically on the formation of the viral RNA polymerase.

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