In vitro replication of cowpea mosaic virus RNA. II. Solubilization of membrane-bound replicase and the partial purification of the solubilized enzyme.

1976 ◽  
Vol 17 (3) ◽  
pp. 679-685 ◽  
Author(s):  
P Zabel ◽  
I Jongen-Neven ◽  
A Van Krammen
Keyword(s):  
Mosaic Virus ◽  
Partial Purification ◽  
Cowpea Mosaic Virus ◽  
In Vitro Replication ◽  
Virus Rna ◽  
Solubilized Enzyme ◽  
Membrane Bound

Solubilization and partial purification of particulate catechol-O-methyltransferase from rat liver

1977 ◽  
Vol 55 (10) ◽  
pp. 1108-1113 ◽  
Author(s):  
J. H. Tong ◽  
A. D'Iorio
Keyword(s):  
Rat Liver ◽  
Partial Purification ◽  
Catecholamine Metabolism ◽  
Ph Profile ◽  
Significant Difference ◽  
Solubilized Enzyme ◽  
Relationship Of ◽  
The Relationship ◽  
Substrate Affinities

Particulate catechol-O-methyltransferase (COMT) from rat liver has been solubilized by acetone treatment and partially purified. Results from the present study demonstrate that the solubilized, partially purified enzyme is similar to the cytosol COMT with respect to molecular weight, pH profile, sensitivity toward inhibitors, Mg2+ requirement, and substrate affinities. However, a comparison of the crude particulate COMT and the solubilized enzyme shows that there is a significant difference in their affinity for catechol substrates. This finding suggests that membrane protein and (or) lipid components may play an important role in catecholamine metabolism. The relationship of particulate COMT to [3H]norepinephrine binding was investigated. No correlation between the COMT and [3H]norepinephrine binding activities was observed in vitro.

scholarly journals Enhancing effect of the 3′-untranslated region of tobacco mosaic virus RNA on protein synthesis in vitro

FEBS Letters ◽  
1994 ◽  
Vol 354 (3) ◽  
pp. 271-273 ◽  
Author(s):  
Vladimir V. Zeyenko ◽  
Lyubov A. Ryabova ◽  
Daniel R. Gallie ◽  
Alexander S. Spirin
Keyword(s):  
Protein Synthesis ◽  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Untranslated Region ◽  
Enhancing Effect ◽  
Virus Rna
Sign in / Sign up

Export Citation Format

Share Document