Host genetic background effect on the frequency of mouse mammary tumor virus-induced rearrangements of the int-1 and int-2 loci in mouse mammary tumors.

Morphological, biological, and immunological studies indicate a possible interrelationship between mouse mammary tumor virus and leukemia virus in the development of mouse mammary tumors. Electron microscope studies have shown the presence of both mouse mammary tumor virus (type B) particles and mouse leukemia virus (type C) particles in mouse milk, in tissues, and in tissue cultures from spontaneous and induced mouse mammary tumors (Dmochowski, L., et; al.: Carcinogenesis, A Broad Critique, Williams and Wilkins Co., Baltimore, p.211, 1967;., Dmochowski, L., et al.: J.Nat. Cancer Inst., 40:1339, 1968).

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A New Common Integration Site, Int7, for the Mouse Mammary Tumor Virus in Mouse Mammary Tumors Identifies a Gene Whose Product Has Furin-Like and Thrombospondin-Like Sequences

Journal of Virology ◽

10.1128/jvi.79.15.10093-10096.2005 ◽

2005 ◽

Vol 79 (15) ◽

pp. 10093-10096 ◽

Cited By ~ 34

Author(s):

William Lowther ◽

Korah Wiley ◽

Gilbert H. Smith ◽

Robert Callahan

Keyword(s):

Mammary Tumor ◽

Transcription Initiation ◽

Mouse Mammary Tumor Virus ◽

Integration Site ◽

Mammary Tumors ◽

Mammary Tumor Virus ◽

Mouse Mammary Tumor ◽

Mouse Mammary Tumors ◽

Tumor Virus ◽

Common Integration Site

ABSTRACT A novel common integration site for the mouse mammary tumor virus (MMTV) was identified (designated Int7) in five independently arising mouse mammary tumors. The insertion sites all cluster within a 1-kb region that is 2 to 3 kb 5′ of the transcription initiation site of a gene, 2610028F08RIK, whose gene product contains furin-like and thrombospondin-like sequences. Expression of Int7 is normally very low or silent during various stages of mammary gland development, but MMTV integration at this site results in the activation of high steady-state levels of expression of the gene. These five tumors were also found to have two or three additional viral insertions, which in each case occurred flanking a member of either the Wnt and/or FGF gene family. Reverse transcriptase PCR results demonstrated that each of the viral insertions led to elevated expression of the presumed target flanking genes.

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Genes affected by mouse mammary tumor virus (MMTV) proviral insertions in mouse mammary tumors are deregulated or mutated in primary human mammary tumors

Oncotarget ◽

10.18632/oncotarget.682 ◽

2012 ◽

Vol 3 (11) ◽

pp. 1320-1334 ◽

Cited By ~ 19

Author(s):

Robert Callahan ◽

Uma Mudunuri ◽

Sharon Bargo ◽

Ahmed Raafat ◽

David McCurdy ◽

...

Keyword(s):

Mammary Tumor ◽

Mouse Mammary Tumor Virus ◽

Mammary Tumors ◽

Mammary Tumor Virus ◽

Mouse Mammary Tumor ◽

Mouse Mammary Tumors ◽

Tumor Virus ◽

Human Mammary Tumors

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Electron Microscopy of the Nucleic Acid of Mouse Mammary Tumor Virus

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100099854 ◽

1968 ◽

Vol 26 ◽

pp. 22-23

Author(s):

N. H. Sarkar ◽

Dan H. Moore

Keyword(s):

Mammary Tumor ◽

Mouse Mammary Tumor Virus ◽

Ammonium Acetate ◽

Dry Weight ◽

Mouse Tumor ◽

Mammary Tumor Virus ◽

Rna Molecules ◽

Mouse Mammary Tumor ◽

Tumor Virus ◽

The Subject

Mouse mammary tumor virus (MTV) is believed to contain about 0.8% single stranded ribonucleic acid (RNA). This value of RNA content was estimated on a dry weight basis. The subject of this report is an attempt to visualize the RNA molecules of MTV particles.MTV particles were isolated from RIII mouse (tumor incidence approximately 80%) milk according to the method described by Lyons and Moore. Purified virions from 5 ml of milk were finally suspended in 0.2 ml of PBS, pH 7.4 and was mixed with an equal volume of pronase (5 mg/ml). This mixture was incubated at 37°C for an hour. RNA was extracted three times using freshly prepared cold phenol. It was then treated three times with cold ethyl ether to remove any trace of phenol. The RNA thus extracted was divided into two parts. One part was diluted four fold with 8M urea to avoid aggregation of the molecules. The other part was left untreated. Both samples were then mixed with an equal volume of 1M ammonium acetate, adjusted to pH 8.0 with NH3 containing chymotrypsin at a concentration of 0.01%.

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