scholarly journals Measles Virus DNA Vaccination: Antibody Isotype Is Determined by the Method of Immunization and by the Nature of both the Antigen and the Coimmunized Antigen

1998 ◽  
Vol 72 (3) ◽  
pp. 2516-2518 ◽  
Author(s):  
Alicia I. Cardoso ◽  
Nathalie Sixt ◽  
Agnes Vallier ◽  
Joel Fayolle ◽  
Robin Buckland ◽  
...  
Keyword(s):  
Immune Responses ◽  
Measles Virus ◽  
Dna Vaccination ◽  
Class I ◽  
Gene Gun ◽  
Cytotoxic Lymphocyte ◽  
Antibody Isotype ◽  
Igg2a Antibody ◽  
Igg1 Isotype

ABSTRACT Plasmids encoding the measles virus hemagglutinin (HA) and nucleoprotein (NP) proteins inoculated into the skin of BALB/c mice by the gene gun method induced both humoral and cytotoxic lymphocyte class I-restrict- ed immune responses. Although intramuscular immunization induces the immunoglobulin G2a (IgG2a) antibody isotype for both antigens, with gene gun immunization, the NP still generated mainly IgG2a and the major isotype induced by the HA was IgG1. Interestingly, gene gun coimmunization of HA and NP plasmids resulted in a dominant IgG1 HA response and the switching of antibodies generated against the NP to the IgG1 isotype.

Induction of immunodeficiency virus-specific immune responses in rhesus monkeys following gene gun-mediated DNA vaccination

1996 ◽  
Vol 25 (3) ◽  
pp. 236-241 ◽  
Author(s):  
Deborah Heydenburg Fuller ◽  
Michael Murphey-Corb ◽  
Janice Clements ◽  
Susan Barnett ◽  
Joel R. Haynes
Keyword(s):  
Immune Responses ◽  
Rhesus Monkeys ◽  
Dna Vaccination ◽  
Gene Gun ◽  
Immunodeficiency Virus

scholarly journals Coadministration of DNA Encoding Interleukin-6 and Hemagglutinin Confers Protection from Influenza Virus Challenge in Mice

1998 ◽  
Vol 72 (2) ◽  
pp. 1704-1708 ◽  
Author(s):  
Diane L. Larsen ◽  
Naomi Dybdahl-Sissoko ◽  
Martha W. McGregor ◽  
Robert Drape ◽  
Veronica Neumann ◽  
...  
Keyword(s):  
Immune Responses ◽  
Interleukin 6 ◽  
Influenza A ◽  
Dna Vaccination ◽  
Gene Gun ◽  
Dna Encoding ◽  
Challenge Virus ◽  
Virus Challenge ◽  
Equine Influenza ◽  
Homologous Challenge

ABSTRACT This study was conducted to investigate whether Accell gene gun coadministration of DNA encoding human interleukin-6 (IL-6) would enhance protective immune responses in mice to an equine influenza A virus hemagglutinin (HA) DNA vaccine. Mice that received HA DNA alone exhibited accelerated clearance of homologous challenge virus but were not protected from infection. In contrast, mice that received both HA and IL-6 DNA had no detectable virus in their lungs after challenge. These results strongly support the use of IL-6 as a cytokine adjuvant in DNA vaccination.

Priming of measles virus-specific humoral- and cellular-immune responses in macaques by DNA vaccination

Vaccine ◽  
2002 ◽  
Vol 20 (16) ◽  
pp. 2022-2026 ◽  
Author(s):  
Koert J. Stittelaar ◽  
Rik L. de Swart ◽  
Helma W. Vos ◽  
Geert van Amerongen ◽  
Nathalie Sixt ◽  
...  
Keyword(s):  
Immune Responses ◽  
Measles Virus ◽  
Dna Vaccination ◽  
Cellular Immune Responses ◽  
Cellular Immune

scholarly journals Canine Distemper Virus DNA Vaccination Induces Humoral and Cellular Immunity and Protects against a Lethal Intracerebral Challenge

1998 ◽  
Vol 72 (11) ◽  
pp. 8472-8476 ◽  
Author(s):  
Nathalie Sixt ◽  
Alicia Cardoso ◽  
Agnès Vallier ◽  
Joël Fayolle ◽  
Robin Buckland ◽  
...  
Keyword(s):  
Canine Distemper Virus ◽  
Lethal Dose ◽  
Serum Antibody ◽  
Dna Vaccination ◽  
Gene Gun ◽  
Canine Distemper ◽  
Cytotoxic Lymphocyte ◽  
Th2 Response ◽  
Humoral And Cellular Immunity ◽  
Igg2a Response

ABSTRACT We have studied the immune responses to the two glycoproteins of the Morbillivirus canine distemper virus (CDV) after DNA vaccination of BALB/c mice. The plasmids coding for both CDV hemagglutinin (H) and fusion protein (F) induce high levels of antibodies which persist for more than 6 months. Intramuscular inoculation of the CDV DNA induces a predominantly immunoglobulin G2a (IgG2a) response (Th1 response), whereas gene gun immunization with CDV H evokes exclusively an IgG1 response (Th2 response). In contrast, the CDV F gene elicited a mixed, IgG1 and IgG2a response. Mice vaccinated (by gene gun) with either the CDV H or F DNA showed a class I-restricted cytotoxic lymphocyte response. Immunized mice challenged intracerebrally with a lethal dose of a neurovirulent strain of CDV were protected. However, approximately 30% of the mice vaccinated with the CDV F DNA became obese in the first 2 months following the challenge. This was not correlated with the serum antibody levels.

Immunologic significance of HLA class I genes in measles virus-specific IFN-γ and IL-4 cytokine immune responses

2005 ◽  
Vol 57 (11) ◽  
pp. 828-836 ◽  
Author(s):  
Inna G. Ovsyannikova ◽  
Jenna E. Ryan ◽  
Robert A. Vierkant ◽  
V. Shane Pankratz ◽  
Robert M. Jacobson ◽  
...  
Keyword(s):  
Immune Responses ◽  
Measles Virus ◽  
Hla Class I ◽  
Class I ◽  
Ifn Γ

Factors determining antibody isotype in measles virus DNA vaccination

1998 ◽  
Vol 149 (1) ◽  
pp. 99-100
Author(s):  
I. Cardoso ◽  
N. Sixt ◽  
A. Valuer ◽  
J. Fayolle ◽  
R. Buckland ◽  
...  
Keyword(s):  
Measles Virus ◽  
Dna Vaccination ◽  
Antibody Isotype

Upregulation of HLA class I and antiviral immune responses in graves’ disease

2020 ◽  
Author(s):  
Therese Weider ◽  
Sarah Richardson ◽  
Noel G. Morgan ◽  
Trond H. Paulsen ◽  
Knut Dahl-Jørgensen ◽  
...  
Keyword(s):  
Immune Responses ◽  
Hla Class I ◽  
Class I ◽  
Graves Disease

scholarly journals An ultra-low-cost electroporator with microneedle electrodes (ePatch) for SARS-CoV-2 vaccination

2021 ◽  
Vol 118 (45) ◽  
pp. e2110817118
Author(s):  
Dengning Xia ◽  
Rui Jin ◽  
Gaurav Byagathvalli ◽  
Huan Yu ◽  
Ling Ye ◽  
...  
Keyword(s):  
Immune Responses ◽  
Large Scale ◽  
Low Cost ◽  
Electrode Array ◽  
Dna Vaccination ◽  
Intradermal Injection ◽  
Antibody Responses ◽  
Transient Effects ◽  
Electric Pulses ◽  
High Electric Field Strength

Vaccination against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and other pathogens with pandemic potential requires safe, protective, inexpensive, and easily accessible vaccines that can be developed and manufactured rapidly at a large scale. DNA vaccines can achieve these criteria, but induction of strong immune responses has often required bulky, expensive electroporation devices. Here, we report an ultra-low-cost (<1 USD), handheld (<50 g) electroporation system utilizing a microneedle electrode array (“ePatch”) for DNA vaccination against SARS-CoV-2. The low cost and small size are achieved by combining a thumb-operated piezoelectric pulser derived from a common household stove lighter that emits microsecond, bipolar, oscillatory electric pulses and a microneedle electrode array that targets delivery of high electric field strength pulses to the skin’s epidermis. Antibody responses against SARS-CoV-2 induced by this electroporation system in mice were strong and enabled at least 10-fold dose sparing compared to conventional intramuscular or intradermal injection of the DNA vaccine. Vaccination was well tolerated with mild, transient effects on the skin. This ePatch system is easily portable, without any battery or other power source supply, offering an attractive, inexpensive approach for rapid and accessible DNA vaccination to combat COVID-19, as well as other epidemics.

scholarly journals Synergistic interaction between interleukin-12 and steel factor in support of proliferation of murine lymphohematopoietic progenitors in culture

Blood ◽  
1994 ◽  
Vol 83 (1) ◽  
pp. 92-98 ◽  
Author(s):  
F Hirayama ◽  
N Katayama ◽  
S Neben ◽  
D Donaldson ◽  
EB Nickbarg ◽  
...  
Keyword(s):  
Immune Responses ◽  
Colony Formation ◽  
Killer Cell ◽  
Interleukin 12 ◽  
Cytotoxic Lymphocyte ◽  
Steel Factor ◽  
Maturation Factor ◽  
Secondary Culture ◽  
Stimulatory Factor ◽  
Marrow Cells

We have investigated the effects of interleukin (IL)-12 (natural killer cell stimulatory factor/cytotoxic lymphocyte maturation factor) on the proliferation of murine myeloid and lymphohematopoietic progenitors in methylcellulose culture. In the presence of erythropoietin (Ep), IL-12 alone failed to support colony formation by mononuclear and enriched marrow cells of normal mice. Steel factor (SF) alone supported primarily formation of granulocyte/macrophage (GM) colony formation. However, the combination of the two cytokines yielded a significant number of multilineage colonies. When tested on marrow cells from 5- fluorouracil (5-FU)-treated mice, the combination of IL-12 and SF, but not the single factors, was effective in support of formation of various types of colonies. Approximately 25% of these colonies yielded pre-B-cell colonies when replated in secondary culture containing SF and IL-7, indicating that IL-12 can interact with SF in supporting the development of primitive lymphohematopoietic progenitors. These results demonstrate that IL-12, a cytokine believed to be involved in the development of cell-mediated immune responses, has a wider range of activity, including committed myeloid and multipotent lymphohematopoietic progenitors.

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