scholarly journals PEGylation of a Vesicular Stomatitis Virus G Pseudotyped Lentivirus Vector Prevents Inactivation in Serum

2004 ◽  
Vol 78 (2) ◽  
pp. 912-921 ◽  
Author(s):  
Maria A. Croyle ◽  
Shellie M. Callahan ◽  
Alberto Auricchio ◽  
Gregg Schumer ◽  
Klause D. Linse ◽  
...  
Keyword(s):  
Ethylene Glycol ◽  
Vesicular Stomatitis Virus ◽  
Transduction Efficiency ◽  
Virus Particles ◽  
Pharmacokinetic Profiles ◽  
Vesicular Stomatitis ◽  
Poly Ethylene ◽  
Lentivirus Vectors

ABSTRACT One disadvantage of vesicular stomatitis virus G (VSV-G) pseudotyped lentivirus vectors for clinical application is inactivation of the vector by human serum complement. To prevent this, monomethoxypoly(ethylene) glycol was conjugated to a VSV-G-human immunodeficiency virus vector expressing Escherichia coli beta-galactosidase. The modification did not affect transduction efficiency in vitro and protected the vector from inactivation in complement-active human and mouse sera. Blood from mice dosed intravenously with either the unmodified or the PEGylated virus particles was assayed for active vector by a limiting-dilution assay to evaluate transduction efficiency and for p24, an indicator of the total number of virus particles present. PEGylation extended the circulation half-life of active vector by a factor of 5 and reduced the rate of vector inactivation in the serum by a factor of 1,000. Pharmacokinetic profiles for the total number of virus particles present in the circulation were unaffected by PEGylation. Modification of the vector with poly(ethylene) glycol significantly enhanced transduction efficiency in the bone marrow and in the spleen 14 days after systemic administration of the virus. These results, in concert with the pharmacokinetic profiles, indicate that PEGylation does protect the virus from inactivation in the serum and, as a result, improves the transduction efficiency of VSV-G pseudotyped lentivirus vectors in susceptible organs in vivo.

scholarly journals 160. Truncated Vesicular Stomatitis Virus G- Protein Improves Baculovirus Transduction Efficiency In Vitro and In Vivo

2006 ◽  
Vol 13 ◽  
pp. S63
Author(s):  
Minna U. Kaikkonen ◽  
Jani K. Raty ◽  
Kari J. Airenne ◽  
Thomas Wirth ◽  
Tommi Heikura ◽  
...  
Keyword(s):  
G Protein ◽  
Vesicular Stomatitis Virus ◽  
Transduction Efficiency ◽  
Vesicular Stomatitis

scholarly journals Truncated vesicular stomatitis virus G protein improves baculovirus transduction efficiency in vitro and in vivo

Gene Therapy ◽  
2005 ◽  
Vol 13 (4) ◽  
pp. 304-312 ◽  
Author(s):  
M U Kaikkonen ◽  
J K Räty ◽  
K J Airenne ◽  
T Wirth ◽  
T Heikura ◽  
...  
Keyword(s):  
G Protein ◽  
Vesicular Stomatitis Virus ◽  
Transduction Efficiency ◽  
Vesicular Stomatitis

scholarly journals Local Toxicity of Topically Administrated Thermoresponsive Systems: In Vitro Studies with In Vivo Correlation

2018 ◽  
Vol 47 (3) ◽  
pp. 426-432 ◽  
Author(s):  
Sivan Yogev ◽  
Ayelet Shabtay-Orbach ◽  
Abraham Nyska ◽  
Boaz Mizrahi
Keyword(s):  
Block Copolymer ◽  
Ethylene Glycol ◽  
Medical Devices ◽  
Poly Lactic Acid ◽  
Poly Ethylene Glycol ◽  
Thermoresponsive Polymers ◽  
Wide Range ◽  
Poly Ethylene

Thermoresponsive materials have the ability to respond to a small change in temperature—a property that makes them useful in a wide range of applications and medical devices. Although very promising, there is only little conclusive data about the cytotoxicity and tissue toxicity of these materials. This work studied the biocompatibility of three Food and Drug Administration approved thermoresponsive polymers: poly( N-isopropyl acrylamide), poly(ethylene glycol)-poly(propylene glycol)-poly(ethylene glycol) tri-block copolymer, and poly(lactic acid-co-glycolic acid) and poly(ethylene glycol) tri-block copolymer. Fibroblast NIH 3T3 and HaCaT keratinocyte cells were used for the cytotoxicity testing and a mouse model for the in vivo evaluation. In vivo results generally showed similar trends as the results seen in vitro, with all tested materials presenting a satisfactory biocompatibility in vivo. pNIPAM, however, showed the highest toxicity both in vitro and in vivo, which was explained by the release of harmful monomers and impurities. More data focusing on the biocompatibility of novel thermoresponsive biomaterials will facilitate the use of existing and future medical devices.

In vivo and in vitro degradation of poly(ether ester) block copolymers based on poly(ethylene glycol) and poly(butylene terephthalate)

Biomaterials ◽  
2004 ◽  
Vol 25 (2) ◽  
pp. 247-258 ◽  
Author(s):  
A.A. Deschamps ◽  
A.A. van Apeldoorn ◽  
H. Hayen ◽  
J.D. de Bruijn ◽  
U. Karst ◽  
...  
Keyword(s):  
Block Copolymers ◽  
Ethylene Glycol ◽  
Poly Ethylene Glycol ◽  
In Vitro Degradation ◽  
Butylene Terephthalate ◽  
Poly Ethylene ◽  
Ether Ester

In vitro and in vivo study of poly(ethylene glycol) conjugated ketoprofen to extend the duration of action

2007 ◽  
Vol 341 (1-2) ◽  
pp. 50-57 ◽  
Author(s):  
Hoo-Kyun Choi ◽  
Myung-Kwan Chun ◽  
Se Hee Lee ◽  
Mee Hee Jang ◽  
Hee Doo Kim ◽  
...  
Keyword(s):  
Ethylene Glycol ◽  
In Vivo Study ◽  
Poly Ethylene Glycol ◽  
Duration Of Action ◽  
Poly Ethylene

Stabilization of L-Asparaginase Modified with Comb-Shaped Poly(ethylene glycol) Derivatives, in vivo and in vitro

1994 ◽  
Vol 5 (4) ◽  
pp. 283-286 ◽  
Author(s):  
Yoh Kodera ◽  
Taichi Sekine ◽  
Tohru Yasukohchi ◽  
Yoshihiro Kiriu ◽  
Misao Hiroto ◽  
...  
Keyword(s):  
Ethylene Glycol ◽  
Poly Ethylene Glycol ◽  
Poly Ethylene ◽  
Ethylene Glycol Derivatives

In Vitro and in Vivo Performance of Porcine Islets Encapsulated in Interfacially Photopolymerized Poly(Ethylene Glycol) Diacrylate Membranes

1999 ◽  
Vol 8 (3) ◽  
pp. 293-306 ◽  
Author(s):  
Gregory M. Cruise ◽  
Orion D. Hegre ◽  
Francis V. Lamberti ◽  
Steven R. Hager ◽  
Ron Hill ◽  
...  
Keyword(s):  
Ethylene Glycol ◽  
Poly Ethylene Glycol ◽  
Glycol Diacrylate ◽  
Porcine Islets ◽  
Poly Ethylene
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