Imparting Temperature Sensitivity and Attenuation in Ferrets to A/Puerto Rico/8/34 Influenza Virus by Transferring the Genetic Signature for Temperature Sensitivity from Cold-Adapted A/Ann Arbor/6/60
2004 ◽
Vol 78
(2)
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pp. 995-998
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ABSTRACT The four temperature-sensitive (ts) loci identified in the PB1 and PB2 gene segments of cold-adapted A/Ann Arbor/6/60 influenza virus, the master donor virus for influenza A virus (MDV-A) FluMist vaccines, were introduced into a divergent A/Puerto Rico/8/34 influenza virus strain. Recombinant A/Puerto Rico/8/34 virus with these four introduced ts loci exhibited both ts and att phenotypes similar to those of MDV-A, which could be used as a donor virus for manufacturing large quantities of inactivated influenza virus vaccine against potential pandemic strains.
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2004 ◽
Vol 158
(1)
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pp. 65
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2003 ◽
Vol 158
(4)
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pp. 305-311
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2006 ◽
Vol 25
(10)
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pp. 860-869
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2018 ◽
Vol 218
(6)
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pp. 856-867
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