Kaposi's Sarcoma-Associated Herpesvirus Latency-Associated Nuclear Antigen Induces a Strong Bend on Binding to Terminal Repeat DNA
2005 ◽
Vol 79
(21)
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pp. 13829-13836
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Keyword(s):
ABSTRACT During latency, the Kaposi's sarcoma-associated herpesvirus genome is maintained as a circular episome, replicating in synchrony with host chromosomes. Replication requires the latency-associated nuclear antigen (LANA) and an origin of latent DNA replication located in the viral terminal repeats, consisting of two LANA binding sites (LBSs) and a GC-rich sequence. Here, we show that the recruitment of a LANA dimer to high-affinity site LBS-1 bends DNA by 57° and towards the major groove. The cooccupancy of LBS-1 and lower-affinity LBS-2 induces a symmetrical bend of 110°. By changing the origin architecture, LANA may help to assemble a specific nucleoprotein structure important for the initiation of DNA replication.
2004 ◽
Vol 85
(4)
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pp. 843-855
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2004 ◽
Vol 78
(1)
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pp. 294-301
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2003 ◽
Vol 84
(6)
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pp. 1451-1462
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2002 ◽
Vol 76
(22)
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pp. 11677-11687
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