Induction of yeast histone genes by stimulation of stationary-phase cells

In the cell cycle of the budding yeast Saccharomyces cerevisiae, expression of the histone genes H2A and H2B of the TRT1 and TRT2 loci is regulated by the performance of "start," the step that also regulates the cell cycle. Here we show that histone production is also subject to an additional form of regulation that is unrelated to the mitotic cell cycle. Expression of histone genes, as assessed by Northern (RNA) analysis, was shown to increase promptly after the stimulation, brought about by fresh medium, that activates stationary-phase cells to reenter the mitotic cell cycle. The use of a yeast mutant that is conditionally blocked in the resumption of proliferation at a step that is not part of the mitotic cell cycle (M.A. Drebot, G.C. Johnston, and R.A. Singer, Proc. Natl. Acad. Sci. 84:7948, 1987) showed that this increased gene expression that occurs upon stimulation of stationary-phase cells took place in the absence of DNA synthesis and without the performance of start. This stimulation-specific gene expression was blocked by the mating pheromone alpha-factor, indicating that alpha-factor directly inhibits expression of these histone genes, independently of start.

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Regulation of proliferation by the budding yeast Saccharomyces cerevisiae

Biochemistry and Cell Biology ◽

10.1139/o90-060 ◽

1990 ◽

Vol 68 (2) ◽

pp. 427-435 ◽

Cited By ~ 3

Author(s):

Gerald C. Johnston ◽

Richard A. Singer

Keyword(s):

Saccharomyces Cerevisiae ◽

Cell Cycle ◽

Stationary Phase ◽

Budding Yeast ◽

Developmental State ◽

Mitotic Cell ◽

Mitotic Cell Cycle ◽

Proliferating Cells ◽

Yeast Saccharomyces Cerevisiae ◽

Regulation Of Proliferation

Mutations in the budding yeast Saccharomyces cerevisiae define regulatory activities both for the mitotic cell cycle and for resumption of proliferation from the quiescent stationary-phase state. In each case, the regulation of proliferation occurs in the prereplicative interval that precedes the initiation of DNA replication. This regulation is particularly responsive to the nutrient environment and the biosynthetic capacity of the cell. Mutations in components of the cAMP-mediated effector pathway and in components of the biosynthetic machinery itself affect regulation of proliferation within the mitotic cell cycle. In the extreme case of nutrient starvation, cells cease proliferation and enter stationary phase. Mutations in newly defined genes prevent stationary-phase cells from reentering the mitotic cell cycle, but have no effect on proliferating cells. Thus stationary phase represents a unique developmental state, with requirements to resume proliferation that differ from those for the maintenance of proliferation in the mitotic cell cycle.Key words: Saccharomyces cerevisiae, cell cycle, growth, cAMP, stationary phase.

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Integrative analyses of gene expression profile reveal potential crucial roles of mitotic cell cycle and microtubule cytoskeleton in pulmonary artery hypertension

BMC Medical Genomics ◽

10.1186/s12920-020-00740-x ◽

2020 ◽

Vol 13 (1) ◽

Author(s):

Jing Luo ◽

Haiyan Li ◽

Zhenwei Liu ◽

Chenlu Li ◽

Ruochen Wang ◽

...

Keyword(s):

Gene Expression ◽

Cell Cycle ◽

Pulmonary Artery ◽

Gene Expression Profile ◽

Expression Profile ◽

Mitotic Cell ◽

Pulmonary Artery Hypertension ◽

Mitotic Cell Cycle ◽

Microtubule Cytoskeleton ◽

Integrative Analyses

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Integrative analyses of gene expression profile reveal potential crucial roles of mitotic cell cycle and microtubule cytoskeleton in pulmonary artery hypertension

10.21203/rs.2.16207/v2 ◽

2020 ◽

Author(s):

Jing Luo ◽

Zhenwei Liu ◽

Chenlu Li ◽

Ruochen Wang ◽

Jinxia Fang ◽

...

Keyword(s):

Gene Expression ◽

Cell Cycle ◽

Gene Expression Profile ◽

Expression Profile ◽

Mitotic Cell ◽

Mitotic Cell Cycle ◽

Public Database ◽

Hub Genes ◽

Microtubule Cytoskeleton ◽

Integrative Analyses

Abstract Background: Pulmonary arterial hypertension (PAH) is a life-threatening condition that gets worse over time. Despite advances in the development of strategies for treating PAH, prognosis of the disease remains unsatisfactory, especially for advanced PAH. The aim of this study was to explore potential crucial genes and pathways associated with PAH based on integrative analyses of gene expression and shed light on the identification of biomarker for PAH. Results: Gene expression profile of pulmonary tissues from 27 PAH patients and 22 normal controls were downloaded from public database (GSE53408 and GSE113439). A total of 521 differentially expressed genes (DEGs), including 432 up-regulated DEGs and 89 down-regulated DEGs were identified using “limma” package in R. Functional enrichment analysis showed that these DEGs were mainly enriched in mitotic cell cycle process, mitotic cell cycle and microtubule cytoskeleton organization. Moreover, five key genes (CDK1, SMC2, SMC4, KIF23, and CENPE) were identified based on the comprehensive evaluation of protein-protein interaction (PPI) network analysis, modular analysis and cytohubba’s analysis, then further validated in another transcriptomic data set associated with PAH from public database (GSE33463). Furthermore, these hub genes were mainly enriched in promoting mitotic cell cycle process, which may be closely associated with the pathogenesis of PAH. We also found that the predicted micro-RNAs (miRNAs) targeting these hub genes were found to be enriched in TGF-β and Hippo signaling pathway. Conclusion:These findings are expected to gain a further insight into the development of PAH and provide a promising index for the detection of PAH.

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Integrative analyses of gene expression profile reveal potential crucial roles of mitotic cell cycle and microtubule cytoskeleton in pulmonary artery hypertension

10.21203/rs.2.16207/v1 ◽

2019 ◽

Author(s):

Jing Luo ◽

Zhenwei Liu ◽

Chenlu Li ◽

Ruochen Wang ◽

Jinxia Fang ◽

...

Keyword(s):

Gene Expression ◽

Cell Cycle ◽

Gene Expression Profile ◽

Expression Profile ◽

Mitotic Cell ◽

Functional Enrichment ◽

Mitotic Cell Cycle ◽

Public Database ◽

Hub Genes ◽

Microtubule Cytoskeleton

Abstract Background: Pulmonary arterial hypertension (PAH) is a life-threatening condition that gets worse over time. Despite advances in the development of strategies for treating PAH, prognosis of the disease remains unsatisfactory, especially for advanced PAH. The aim of this study was to explore potential crucial genes and pathways associated with PAH that can be used as potential biomarkers for early diagnosis.Results: Gene expression profile of pulmonary tissues from 27 PAH patients and 22 normal controls were downloaded from public database (GSE53408 and GSE113439). A total of 521 differentially expressed genes (DEGs), including 432 up-regulated DEGs and 89 down-regulated DEGs were identified using “limma” package in R. Functional enrichment analysis showed that these DEGs were mainly enriched in mitotic cell cycle process, mitotic cell cycle and microtubule cytoskeleton organization. Moreover, five key genes (CDK1, SMC2, SMC4, KIF23, and CENPE) were identified based on the comprehensive evaluation of PPI analysis, modular analysis and cytohubba’s analysis, then further validated in another transcriptomic data set associated with PAH from public database (GSE33463). Furthermore, these hub genes were mainly enriched in promoting mitotic cell cycle process, which may be closely associated with the pathogenesis of PAH. We also found that the predicted miRNAs targeting these hub genes were found to be enriched in TGF-β and Hippo signaling pathway.Conclusion: These findings are expected to gain a further insight into the development of PAH and provide a promising index for the detection of PAH.

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Diverse effects of beta-tubulin mutations on microtubule formation and function.

The Journal of Cell Biology ◽

10.1083/jcb.106.6.1997 ◽

1988 ◽

Vol 106 (6) ◽

pp. 1997-2010 ◽

Cited By ~ 204

Author(s):

T C Huffaker ◽

J H Thomas ◽

D Botstein

Keyword(s):

Cell Cycle ◽

Nuclear Migration ◽

Mitotic Cell ◽

Restrictive Temperature ◽

In Vitro Mutagenesis ◽

Mitotic Cell Cycle ◽

Beta Tubulin ◽

Yeast Saccharomyces Cerevisiae ◽

Cytoplasmic Microtubule ◽

Cytoplasmic Microtubules

We have used in vitro mutagenesis and gene replacement to construct five new cold-sensitive mutations in TUB2, the sole gene encoding beta-tubulin in the yeast Saccharomyces cerevisiae. These and one previously isolated tub2 mutant display diverse phenotypes that have allowed us to define the functions of yeast microtubules in vivo. At the restrictive temperature, all of the tub2 mutations inhibit chromosome segregation and block the mitotic cell cycle. However, different microtubule arrays are present in these arrested cells depending on the tub2 allele. One mutant (tub2-401) contains no detectable microtubules, two (tub2-403 and tub2-405) contain greatly diminished levels of both nuclear and cytoplasmic microtubules, one (tub2-104) contains predominantly nuclear microtubules, one (tub2-402) contains predominantly cytoplasmic microtubules, and one (tub2-404) contains prominent nuclear and cytoplasmic microtubule arrays. Using these mutants we demonstrate here that cytoplasmic microtubules are necessary for nuclear migration during the mitotic cell cycle and for nuclear migration and fusion during conjugation; only those mutants that possess cytoplasmic microtubules are able to perform these functions. We also show that microtubules are not required for secretory vesicle transport in yeast; bud growth and invertase secretion occur in cells which contain no microtubules.

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