Identification and characterization of the Egr-1 gene product, a DNA-binding zinc finger protein induced by differentiation and growth signals

1990 ◽  
Vol 10 (5) ◽  
pp. 1931-1939
Author(s):  
X M Cao ◽  
R A Koski ◽  
A Gashler ◽  
M McKiernan ◽  
C F Morris ◽  
...  
Keyword(s):  
Gene Product ◽  
Zinc Finger Protein ◽  
Zinc Fingers ◽  
Early Response ◽  
Cell Fractionation ◽  
Dependent Manner ◽  
Finger Protein ◽  
Identification And Characterization

Egr-1 is an immediate-early response gene induced by diverse signals that initiate growth and differentiation. Its cDNA sequence predicts a protein with zinc fingers. We have generated an antiserum to the Egr-1 gene product and identified it as an 80-kilodalton short-lived protein in serum-stimulated mouse fibroblasts. The rat Egr-1 product has also been identified in nerve growth factor-induced PC12 cells. In addition, we show by cell fractionation and immunocytochemistry that the Egr-1 protein is located in the nucleus. We also demonstrate that it is phosphorylated. In vitro-generated Egr-1 protein binds with high affinity to the sequence CGCCCCCGC in a zinc-dependent manner.

scholarly journals Identification and characterization of the Egr-1 gene product, a DNA-binding zinc finger protein induced by differentiation and growth signals.

1990 ◽  
Vol 10 (5) ◽  
pp. 1931-1939 ◽  
Author(s):  
X M Cao ◽  
R A Koski ◽  
A Gashler ◽  
M McKiernan ◽  
C F Morris ◽  
...  
Keyword(s):  
Gene Product ◽  
Zinc Finger Protein ◽  
Zinc Fingers ◽  
Early Response ◽  
Cell Fractionation ◽  
Dependent Manner ◽  
Finger Protein ◽  
Identification And Characterization

Egr-1 is an immediate-early response gene induced by diverse signals that initiate growth and differentiation. Its cDNA sequence predicts a protein with zinc fingers. We have generated an antiserum to the Egr-1 gene product and identified it as an 80-kilodalton short-lived protein in serum-stimulated mouse fibroblasts. The rat Egr-1 product has also been identified in nerve growth factor-induced PC12 cells. In addition, we show by cell fractionation and immunocytochemistry that the Egr-1 protein is located in the nucleus. We also demonstrate that it is phosphorylated. In vitro-generated Egr-1 protein binds with high affinity to the sequence CGCCCCCGC in a zinc-dependent manner.

Her, a gene required for sexual differentiation in Drosophila, encodes a zinc finger protein with characteristics of ZFY-like proteins and is expressed independently of the sex determination hierarchy

Development ◽  
1998 ◽  
Vol 125 (2) ◽  
pp. 225-235
Author(s):  
H. Li ◽  
B.S. Baker
Keyword(s):  
Sex Determination ◽  
Sexual Differentiation ◽  
Zinc Finger Protein ◽  
Genetic Characterization ◽  
Zinc Fingers ◽  
Molecular Genetic ◽  
Control Female ◽  
Finger Protein ◽  
Molecular Genetic Characterization

The zygotic function of the hermaphrodite (her) gene of Drosophila plays an important role in sexual differentiation. Our molecular genetic characterization of her suggests that her is expressed sex non-specifically and independently of other known sex determination genes and that it acts together with the last genes in the sex determination hierarchy, doublesex and intersex, to control female sexual differentiation. Consistent with such a terminal function in sexual differentiation, her encodes a protein with C2H2-type zinc fingers. The her zinc fingers are atypical and similar to the even-numbered zinc fingers of ZFY and ZFX proteins in humans and other vertebrates.

scholarly journals Structure, expression and in vitro functional characterization of a novel RNA binding zinc finger protein from Xenopus.

1991 ◽  
Vol 10 (10) ◽  
pp. 3087-3093 ◽  
Author(s):  
M. Köster ◽  
U. Kühn ◽  
T. Bouwmeester ◽  
W. Nietfeld ◽  
T. el-Baradi ◽  
...  
Keyword(s):  
Zinc Finger ◽  
Rna Binding ◽  
Zinc Finger Protein ◽  
Functional Characterization ◽  
Finger Protein

Molecular cloning and characterization of a novel RING zinc-finger protein gene up-regulated under in vitro salt stress in cassava

2012 ◽  
Vol 39 (6) ◽  
pp. 6513-6519 ◽  
Author(s):  
Sávio Pinho dos Reis ◽  
Liliane de Souza Conceição Tavares ◽  
Carinne de Nazaré Monteiro Costa ◽  
Aílton Borges Santa Brígida ◽  
Cláudia Regina Batista de Souza
Keyword(s):  
Salt Stress ◽  
Zinc Finger ◽  
Protein Gene ◽  
Zinc Finger Protein ◽  
Zinc Finger Protein Gene ◽  
Finger Protein ◽  
Ring Zinc Finger ◽  
Ring Zinc Finger Protein

Identification and Characterization of Human ZNF274 cDNA, which Encodes a Novel Kruppel-type Zinc-Finger Protein Having Nucleolar Targeting Ability

Genomics ◽  
2000 ◽  
Vol 65 (1) ◽  
pp. 75-80 ◽  
Author(s):  
Kazuhiro Yano ◽  
Nobuhide Ueki ◽  
Tamaki Oda ◽  
Naohiko Seki ◽  
Yasuhiko Masuho ◽  
...  
Keyword(s):  
Zinc Finger ◽  
Zinc Finger Protein ◽  
Finger Protein ◽  
Targeting Ability ◽  
Identification And Characterization
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