scholarly journals Identification and characterization of a novel zinc finger protein (HZF1) gene and its function in erythroid and megakaryocytic differentiation of K562 cells

Leukemia ◽  
2006 ◽  
Vol 20 (6) ◽  
pp. 1109-1116 ◽  
Author(s):  
H Peng ◽  
Z-W Du ◽  
J-W Zhang
Keyword(s):  
Zinc Finger ◽  
Zinc Finger Protein ◽  
K562 Cells ◽  
Megakaryocytic Differentiation ◽  
Finger Protein ◽  
Identification And Characterization

Identification and characterization of a novel Drosophila melanogaster glutathione S-transferase-containing FLYWCH zinc finger protein

Gene ◽  
2004 ◽  
Vol 342 (1) ◽  
pp. 49-56 ◽  
Author(s):  
Mu-Shui Dai ◽  
Xiao-Xin Sun ◽  
Jun Qin ◽  
Sarah M. Smolik ◽  
Hua Lu
Keyword(s):  
Drosophila Melanogaster ◽  
Zinc Finger ◽  
Zinc Finger Protein ◽  
Glutathione S Transferase ◽  
Finger Protein ◽  
Identification And Characterization

Identification and characterization of a novel gene encoding myb-box binding zinc finger protein in Gossypium arboreum

2012 ◽  
Vol 56 (4) ◽  
pp. 641-647 ◽  
Author(s):  
M. Zahur ◽  
A. Maqbool ◽  
M. Irfan ◽  
A. Jamal ◽  
N. Shahid ◽  
...  
Keyword(s):  
Zinc Finger ◽  
Zinc Finger Protein ◽  
Gossypium Arboreum ◽  
Gene Encoding ◽  
Novel Gene ◽  
Finger Protein ◽  
Identification And Characterization

scholarly journals Identification and characterization of a salt stress-inducible zinc finger protein from Festuca arundinacea

2012 ◽  
Vol 5 (1) ◽  
Author(s):  
Ruth C Martin ◽  
Kira Glover-Cutter ◽  
James C Baldwin ◽  
James E Dombrowski
Keyword(s):  
Salt Stress ◽  
Zinc Finger ◽  
Festuca Arundinacea ◽  
Zinc Finger Protein ◽  
Finger Protein ◽  
Identification And Characterization

Identification and Characterization of DPZF, a Novel Human BTB/POZ Zinc Finger Protein Sharing Homology to BCL-6

2001 ◽  
Vol 282 (4) ◽  
pp. 1067-1073 ◽  
Author(s):  
Weiping Zhang ◽  
Jing Mi ◽  
Nan Li ◽  
Lili Sui ◽  
Tao Wan ◽  
...  
Keyword(s):  
Zinc Finger ◽  
Zinc Finger Protein ◽  
Finger Protein ◽  
Identification And Characterization

Identification and Characterization of Human ZNF274 cDNA, which Encodes a Novel Kruppel-type Zinc-Finger Protein Having Nucleolar Targeting Ability

Genomics ◽  
2000 ◽  
Vol 65 (1) ◽  
pp. 75-80 ◽  
Author(s):  
Kazuhiro Yano ◽  
Nobuhide Ueki ◽  
Tamaki Oda ◽  
Naohiko Seki ◽  
Yasuhiko Masuho ◽  
...  
Keyword(s):  
Zinc Finger ◽  
Zinc Finger Protein ◽  
Finger Protein ◽  
Targeting Ability ◽  
Identification And Characterization

Identification and characterization of the zinc finger protein SjZF in Schistosoma japonicum

2018 ◽  
Vol 501 (4) ◽  
pp. 920-926 ◽  
Author(s):  
Ruixiang Zhang ◽  
Jian Li ◽  
Manyu Xiang ◽  
Qinghua Hong ◽  
Bin Xu ◽  
...  
Keyword(s):  
Schistosoma Japonicum ◽  
Zinc Finger ◽  
Zinc Finger Protein ◽  
Finger Protein ◽  
Identification And Characterization

Identification, Characterization of a Novel Zinc Finger Protein (HZF1) Gene and Its Roles in Erythroid Differentiation and Megakaryocyte Differentiation.

Blood ◽  
2005 ◽  
Vol 106 (11) ◽  
pp. 4237-4237
Author(s):  
Jun-Wu Zhang ◽  
Han Peng ◽  
Zhan-Wen Du
Keyword(s):  
Transcription Factors ◽  
Zinc Finger ◽  
Zinc Finger Protein ◽  
K562 Cells ◽  
Erythroid Differentiation ◽  
Structure Characterization ◽  
Megakaryocytic Differentiation ◽  
Cdna Sequences ◽  
Finger Protein ◽  
Zinc Finger Motifs

Abstract A significant number of transcription factors contain evolutionarily conserved zinc finger motifs. The classical C2H2 zinc finger motif, which employs two cysteine and two histidine residues to coordinate a single zinc ion, is a maim type of the zinc finger proteins. Many of the identified C2H2 type zinc protein have been demonstrated to be transcription factors that play important roles in differentiation and development of cells and tissues of higher organisms. In this study, we obtained some novel expression sequence tags (ESTs) containing C2H2 type motifs by reverse transcription-polymerase chain reaction (RT-PCR) using RNAs derived from hemin-induced K562 cells. A cDNA encoding novel zinc finger protein (designed as HZF1) was obtained by screening the human bone marrow cDNA library using one of the ESTs as the probe. The cDNA sequences (2013 nucleotides) have been submitted to the GenBank databases under accession No. AF244088.1). Three transcripts of HZF1 gene were explored by PCR amplification of cDNAs derived from hemin-induced K562 cells. The cDNA sequences (2632 nucleotides) of the longest transcript have been submitted to the GenBank databases under accession No. DQ117529). These transcripts may result from different splicing of the pre-mRNA of HZF1 but the differences between them are only involved in 5′ non-translation region of HZF1 mRNA. BLASTN analysis revealed that HZF1 gene has four exons and three introns. The putative protein consists of 670 amino acid residues including continuous 15 C2H2 and 2 C2RH zinc finger motifs. This structure characterization and the nuclear location of the protein suggest that HZF1 may function as a transcription factor. HZF1 mRNA was detected in ubiquitous tissues and various hematopoietic cell lines. Increased HZF1 mRNA expression was observed following hemin-induction or phorbol myristate acetate (PMA)-induction of K562 cells. Both of the antisence method and RNA interference assay revealed that repression of the intrinsic expression of HZF1 blocked the hemin-induced erythroid differentiation and reduced the PMA-induced megakaryocytic differentiation of K562 cells, which suggested that HZF1 play an important part in erythroid differentiation and megakaryocytic differentiation.

scholarly journals Single amino acid exchanges in separate domains of the Drosophila serendipity delta zinc finger protein cause embryonic and sex biased lethality.

Genetics ◽  
1992 ◽  
Vol 131 (4) ◽  
pp. 905-916 ◽  
Author(s):  
M Crozatier ◽  
K Kongsuwan ◽  
P Ferrer ◽  
J R Merriam ◽  
J A Lengyel ◽  
...  
Keyword(s):  
Amino Acid ◽  
Dna Binding ◽  
Zinc Finger ◽  
Zinc Finger Protein ◽  
Essential Gene ◽  
Larval Instar ◽  
Single Amino Acid ◽  
Isolation And Characterization ◽  
Finger Protein

Abstract The Drosophila serendipity (sry) delta (delta) zinc finger protein is a sequence-specific DNA binding protein, maternally inherited by the embryo and present in nuclei of transcriptionally active cells throughout fly development. We report here the isolation and characterization of four ethyl methanesulfate-induced zygotic lethal mutations of different strengths in the sry delta gene. For the stronger allele, all of the lethality occurs during late embryogenesis or the first larval instar. In the cases of the three weaker alleles, most of the lethality occurs during pupation; moreover, those adult escapers that emerge are sterile males lacking partially or completely in spermatozoa bundles. Genetic analysis of sry delta thus indicates that it is an essential gene, whose continued expression throughout the life cycle, notably during embryogenesis and pupal stage, is required for viability. Phenotypic analysis of sry delta hemizygote escaper males further suggests that sry delta may be involved in regulation of two different sets of genes: genes required for viability and genes involved in gonadal development. All four sry delta alleles are fully rescued by a wild-type copy of sry delta, but not by an additional copy of the sry beta gene, reinforcing the view that, although structurally related, these two genes exert distinct functions. Molecular characterization of the four sry delta mutations revealed that these mutations correspond to single amino acid replacements in the sry delta protein. Three of these replacements map to the same (third out of seven) zinc finger in the carboxy-terminal DNA binding domain; interestingly, none affects the zinc finger consensus residues. The fourth mutation is located in the NH2-proximal part of the protein, in a domain proposed to be involved in specific protein-protein interactions.

Characterization of a family of Arabidopsis zinc finger protein cDNAs

1995 ◽  
Vol 28 (2) ◽  
pp. 267-279 ◽  
Author(s):  
Brian W. Tague ◽  
Howard M. Goodman
Keyword(s):  
Zinc Finger ◽  
Zinc Finger Protein ◽  
Finger Protein
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