Identification ofcut8+andcek1+, a Novel Protein Kinase Gene, Which Complement a Fission Yeast Mutation That Blocks Anaphase:

1994 ◽  
Vol 14 (11) ◽  
pp. 7683.1-7683
Keyword(s):  
Protein Kinase ◽  
Fission Yeast ◽  
Kinase Gene ◽  
Protein Kinase Gene ◽  
Novel Protein

A novel protein kinase gene family in Giardia duodenalis

Gene ◽  
1996 ◽  
Vol 177 (1-2) ◽  
pp. 191-194 ◽  
Author(s):  
Nanhua Chen ◽  
Jacqueline A. Upcroft ◽  
Peter Upcroft
Keyword(s):  
Protein Kinase ◽  
Gene Family ◽  
Giardia Duodenalis ◽  
Kinase Gene ◽  
Protein Kinase Gene ◽  
Novel Protein

scholarly journals Identification of cut8+ and cek1+, a novel protein kinase gene, which complement a fission yeast mutation that blocks anaphase.

1994 ◽  
Vol 14 (9) ◽  
pp. 6361-6371 ◽  
Author(s):  
I Samejima ◽  
M Yanagida
Keyword(s):  
Amino Acid ◽  
Protein Kinase ◽  
Fission Yeast ◽  
Gene Product ◽  
Budding Yeast ◽  
Essential Gene ◽  
Restrictive Temperature ◽  
Kinase Gene ◽  
Yeast Saccharomyces Cerevisiae ◽  
Novel Protein

The fission yeast Schizosaccharomyces pombe [corrected] temperature sensitivity cut8-563 mutation causes chromosome overcondensation and short spindle formation in the absence of sister chromatid separation. The cut8-563 mutation allows cytokinesis before the completion of anaphase, thus producing cells with a cut phenotype. The cut8+ gene product may be required for normal progression of anaphase. Diploidization occurs at the restrictive temperature, and 60 to 70% of the cells surviving after two generations are diploid. These phenotypes are reminiscent of those of budding yeast (Saccharomyces cerevisiae) ctf13 and ctf14 (ndc10) mutations. The cut8+ gene, isolated by complementation of the mutant, predicts a 262-amino-acid protein; the amino and carboxy domains are hydrophilic, while the central domain contains several hydrophobic stretches. It has a weak overall similarity to the budding yeast DBF8 gene product. DBF8 is an essential gene whose mutations result in delay in mitotic progression and chromosome instability. Anti-cut8 antibodies detect a 33-kDa polypeptide. Two multicopy suppressor genes for cut8-563 are identified. They are the cut1+ gene essential for nuclear division, and a new gene (designated cek1+) which encodes a novel protein kinase. The cek1+ gene product is unusually large (1,309 amino acids) and has a 112-amino-acid additional sequence in the kinase domain. The cek1+ gene is not an essential gene. Protein phosphorylation by cek1 may facilitate the progression of anaphase through direct or indirect interaction with the cut8 protein.

Mutational analysis of the fission yeast p34cdc2 protein kinase gene

1993 ◽  
Vol 236-236 (2-3) ◽  
pp. 415-426 ◽  
Author(s):  
Stuart A. MacNeill ◽  
Paul Nurse
Keyword(s):  
Protein Kinase ◽  
Fission Yeast ◽  
Mutational Analysis ◽  
Kinase Gene ◽  
Protein Kinase Gene

Identification of cut8+ and cek1+, a novel protein kinase gene, which complement a fission yeast mutation that blocks anaphase

1994 ◽  
Vol 14 (9) ◽  
pp. 6361-6371
Author(s):  
I Samejima ◽  
M Yanagida
Keyword(s):  
Amino Acid ◽  
Protein Kinase ◽  
Fission Yeast ◽  
Gene Product ◽  
Budding Yeast ◽  
Essential Gene ◽  
Restrictive Temperature ◽  
Kinase Gene ◽  
Yeast Saccharomyces Cerevisiae ◽  
Novel Protein

The fission yeast Schizosaccharomyces pombe [corrected] temperature sensitivity cut8-563 mutation causes chromosome overcondensation and short spindle formation in the absence of sister chromatid separation. The cut8-563 mutation allows cytokinesis before the completion of anaphase, thus producing cells with a cut phenotype. The cut8+ gene product may be required for normal progression of anaphase. Diploidization occurs at the restrictive temperature, and 60 to 70% of the cells surviving after two generations are diploid. These phenotypes are reminiscent of those of budding yeast (Saccharomyces cerevisiae) ctf13 and ctf14 (ndc10) mutations. The cut8+ gene, isolated by complementation of the mutant, predicts a 262-amino-acid protein; the amino and carboxy domains are hydrophilic, while the central domain contains several hydrophobic stretches. It has a weak overall similarity to the budding yeast DBF8 gene product. DBF8 is an essential gene whose mutations result in delay in mitotic progression and chromosome instability. Anti-cut8 antibodies detect a 33-kDa polypeptide. Two multicopy suppressor genes for cut8-563 are identified. They are the cut1+ gene essential for nuclear division, and a new gene (designated cek1+) which encodes a novel protein kinase. The cek1+ gene product is unusually large (1,309 amino acids) and has a 112-amino-acid additional sequence in the kinase domain. The cek1+ gene is not an essential gene. Protein phosphorylation by cek1 may facilitate the progression of anaphase through direct or indirect interaction with the cut8 protein.

Characterization of a protein kinase gene from two Chlorella viruses

1995 ◽  
Vol 35 (3) ◽  
pp. 291-305 ◽  
Author(s):  
Quideng Que ◽  
James L. Van Etten
Keyword(s):  
Protein Kinase ◽  
Kinase Gene ◽  
Protein Kinase Gene

scholarly journals Negative control of Candida albicans filamentation-associated gene expression by essential protein kinase gene KIN28

2017 ◽  
Vol 63 (6) ◽  
pp. 1073-1079 ◽  
Author(s):  
C. A. Woolford ◽  
K. Lagree ◽  
T. Aleynikov ◽  
A. P. Mitchell
Keyword(s):  
Gene Expression ◽  
Candida Albicans ◽  
Protein Kinase ◽  
Negative Control ◽  
Kinase Gene ◽  
Essential Protein ◽  
Protein Kinase Gene
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