Role of nuclear genes in expression of a mitochondrial tRNA gene in Saccharomyces cerevisiae

In yeast mitochondria, most of the isoaccepting species of tyrosyl tRNA are coded by a mitochondrial gene, tyrA. A particular isoaccepting species is coded by a second mitochondrial gene, tyrB. This gene is not expressed in certain strains of yeast which show no deficient phenotype. Genetic crosses between strains expressing or not expressing the tyrB gene demonstrate that expression is controlled by specific nuclear genes and that a mutation of the tyrA gene can be bypassed when the tyrB gene is operative.

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Role of nuclear genes in expression of a mitochondrial tRNA gene in Saccharomyces cerevisiae.

Molecular and Cellular Biology ◽

10.1128/mcb.3.3.371 ◽

1983 ◽

Vol 3 (3) ◽

pp. 371-379

Author(s):

M Wesolowski ◽

C Palleschi ◽

L Frontali ◽

H Fukuhara

Keyword(s):

Saccharomyces Cerevisiae ◽

Trna Gene ◽

Mitochondrial Gene ◽

Nuclear Genes ◽

Yeast Mitochondria ◽

Mitochondrial Trna ◽

Genetic Crosses ◽

Mitochondrial Trna Gene

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Raffelsberger T, Rossmanith W, Thaller-Antlanger H, et al. CPEO associated with a single nucleotide deletion in the mitochondrial tRNA gene.

Journal of Neuro-Ophthalmology ◽

10.1097/00041327-200206000-00046 ◽

2002 ◽

Vol 22 (2) ◽

pp. 144

Author(s):

Kathleen B. Digre

Keyword(s):

Trna Gene ◽

Mitochondrial Trna ◽

Single Nucleotide ◽

Single Nucleotide Deletion ◽

Nucleotide Deletion ◽

Mitochondrial Trna Gene

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Cloning and characterization of LOS1, a Saccharomyces cerevisiae gene that affects tRNA splicing

Molecular and Cellular Biology ◽

10.1128/mcb.7.3.1208-1216.1987 ◽

1987 ◽

Vol 7 (3) ◽

pp. 1208-1216 ◽

Cited By ~ 2

Author(s):

D J Hurt ◽

S S Wang ◽

Y H Lin ◽

A K Hopper

Keyword(s):

Saccharomyces Cerevisiae ◽

Elevated Temperatures ◽

Trna Gene ◽

Single Copy ◽

Molecular Study ◽

Trna Splicing ◽

Intervening Sequences ◽

Copy Gene

Saccharomyces cerevisiae strains carrying los1-1 mutations are defective in tRNA processing; at 37 degrees C, such strains accumulate tRNA precursors which have mature 5' and 3' ends but contain intervening sequences. Strains bearing los1-1 and an intron-containing ochre-suppressing tRNA gene, SUP4(0), also fail to suppress the ochre mutations ade2-1(0) and can1-100(0) at 34 degrees C. To understand the role of the LOS1 product in tRNA splicing, we initiated a molecular study of the LOS1 gene. Two plasmids, YEpLOS1 and YCpLOS1, that complement the los1-1 phenotype were isolated from the YEp24 and YCp50 libraries, respectively. YEpLOS1 and YCpLOS1 had overlapping restriction maps, indicating that the DNA in the overlapping segment could complement los1-1 when present in multiple or single copy. Integration of plasmid DNA at the LOS1 locus confirmed that these clones contained authentic LOS1 sequences. Southern analyses showed that LOS1 is a single copy gene. The locations of the LOS1 gene within YEpLOS1 and YCpLOS1 were determined by deletion and gamma-delta mapping. Two genomic disruptions of the LOS1 gene were constructed, i.e., an insertion of a 1.2-kilobase fragment carrying the yeast URA3 gene, los1::URA3, and a 2.4-kilobase deletion from the LOS1 gene, los1-delta V. Disruption or deletion of most of the LOS1 gene was not lethal; cells carrying the disrupted los1 alleles were viable and had phenotypes similar to those of cells carrying the los1-1 allele. Thus, it appears that the los1 gene product expedites tRNA splicing at elevated temperatures but is not essential for this process.

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