Excision repair functions in Saccharomyces cerevisiae recognize and repair methylation of adenine by the Escherichia coli dam gene
1986 ◽
Vol 6
(10)
◽
pp. 3555-3558
Keyword(s):
Unlike the DNA of higher eucaryotes, the DNA of Saccharomyces cerevisiae (bakers' yeast) is not methylated. Introduction of the Escherichia coli dam gene into yeast cells results in methylation of the N-6 position of adenine. The UV excision repair system of yeast cells specifically responds to the methylation, suggesting that it is capable of recognizing modifications which do not lead to major helix distortion. The UV repair functions examined in this report are involved in the incision step of pyrimidine dimer repair. These observations may have relevance to the rearrangements and recombination events observed when yeast or higher eucaryotic cells are transformed or transfected with DNA grown in E. coli.
1986 ◽
Vol 6
(10)
◽
pp. 3555-3558
◽
Keyword(s):
1989 ◽
Vol 9
(11)
◽
pp. 4767-4776
1989 ◽
Vol 9
(11)
◽
pp. 4767-4776
◽
1989 ◽
Vol 9
(11)
◽
pp. 4777-4788
◽
1994 ◽
Vol 14
(12)
◽
pp. 8071-8077
1989 ◽
Vol 9
(11)
◽
pp. 4777-4788
1994 ◽
Vol 14
(12)
◽
pp. 8071-8077
◽