scholarly journals Subcellular localization of an intermediate filament protein and its mRNA in glial cells.

1989 ◽  
Vol 9 (10) ◽  
pp. 4556-4559 ◽  
Author(s):  
P V Sarthy ◽  
M Fu ◽  
J Huang
Keyword(s):  
Glial Cell ◽  
Intermediate Filament ◽  
Mrna Translation ◽  
Mrna Localization ◽  
Intermediate Filament Protein ◽  
Cell Cytoplasm ◽  
Distal Process ◽  
Spatially Distributed ◽  
Actin Mrna ◽  
Filament Protein

Eucaryotic mRNAs are generally localized in the cell body, where most protein synthesis occurs. We have found that mRNAs encoding the glial intermediate filament protein are spatially distributed in the glial cell cytoplasm close to the location of the glial filaments. Whereas the glial filament protein mRNA was located predominantly in the distal process, actin mRNA was found almost exclusively in the apical portion of the glial cell. This pattern of mRNA localization might provide a mechanism for synthesis of proteins in specific subcellular compartments by mRNA translation locally.

Subcellular localization of an intermediate filament protein and its mRNA in glial cells

1989 ◽  
Vol 9 (10) ◽  
pp. 4556-4559
Author(s):  
P V Sarthy ◽  
M Fu ◽  
J Huang
Keyword(s):  
Glial Cell ◽  
Intermediate Filament ◽  
Mrna Translation ◽  
Mrna Localization ◽  
Intermediate Filament Protein ◽  
Cell Cytoplasm ◽  
Distal Process ◽  
Spatially Distributed ◽  
Actin Mrna ◽  
Filament Protein

Eucaryotic mRNAs are generally localized in the cell body, where most protein synthesis occurs. We have found that mRNAs encoding the glial intermediate filament protein are spatially distributed in the glial cell cytoplasm close to the location of the glial filaments. Whereas the glial filament protein mRNA was located predominantly in the distal process, actin mRNA was found almost exclusively in the apical portion of the glial cell. This pattern of mRNA localization might provide a mechanism for synthesis of proteins in specific subcellular compartments by mRNA translation locally.

Genome wide assessment of mRNA in astrocyte protrusions by direct RNA sequencing reveals mRNA localization for the intermediate filament protein nestin

Glia ◽  
2013 ◽  
Vol 61 (11) ◽  
pp. 1922-1937 ◽  
Author(s):  
Rune Thomsen ◽  
Jonatan Pallesen ◽  
Tina F. Daugaard ◽  
Anders D. Børglum ◽  
Anders L. Nielsen
Keyword(s):  
Rna Sequencing ◽  
Intermediate Filament ◽  
Mrna Localization ◽  
Intermediate Filament Protein ◽  
Genome Wide ◽  
Filament Protein

scholarly journals The Intermediate Filament Protein Vimentin Is a New Target for Epigallocatechin Gallate

2005 ◽  
Vol 280 (17) ◽  
pp. 16882-16890 ◽  
Author(s):  
Svetlana Ermakova ◽  
Bu Young Choi ◽  
Hong Seok Choi ◽  
Bong Seok Kang ◽  
Ann M. Bode ◽  
...  
Keyword(s):  
Intermediate Filament ◽  
Epigallocatechin Gallate ◽  
Intermediate Filament Protein ◽  
Filament Protein

A cDNA from Drosophila melanogaster encodes a lamin C-like intermediate filament protein

1993 ◽  
Vol 104 (4) ◽  
pp. 1263-1272 ◽  
Author(s):  
C.A. Bossie ◽  
M.M. Sanders
Keyword(s):  
Drosophila Melanogaster ◽  
Intermediate Filament ◽  
Blot Analysis ◽  
Polytene Chromosomes ◽  
Intermediate Filament Protein ◽  
Chromosome 2 ◽  
Cross Hybridization ◽  
Intermediate Filament Proteins ◽  
Nuclear Lamins ◽  
Filament Protein

A novel intermediate filament cDNA, pG-IF, has been isolated from a Drosophila melanogaster embryonic expression library screened with a polyclonal antiserum produced against a 46 kDa cytoskeletal protein isolated from Kc cells. This 46 kDa protein is known to be immunologically related to vertebrate intermediate filament proteins. The screen resulted in the isolation of four different cDNA groups. Of these, one has been identified as the previously characterized Drosophila nuclear lamin cDNA, Dm0, and a second, pG-IF, demonstrates homology to Dm0 by cross hybridization on Southern blots. DNA sequence analysis reveals that pG-IF encodes a newly identified intermediate filament protein in Drosophila. Its nucleotide sequence is highly homologous to nuclear lamins with lower homology to cytoplasmic intermediate filament proteins. pG-IF predicts a protein of 621 amino acids with a predicted molecular mass of 69,855 daltons. In vitro transcription and translation of pG-IF yielded a protein with a SDS-PAGE estimated molecular weight of approximately 70 kDa. It contains sequence principles characteristic of class V intermediate filament proteins. Its near neutral pI (6.83) and the lack of a terminal CaaX motif suggests that it may represent a lamin C subtype in Drosophila. In situ hybridization to polytene chromosomes detects one band of hybridization on the right arm of chromosome 2 at or near 51A. This in conjunction with Southern blot analysis of various genomic digests suggests one or more closely placed genes while Northern blot analysis detects two messages in Kc cells.

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