Analysis of Transcriptome of Drosophila melanogaster Strains with Disrupted Control of gypsy Retrotransposon Transposition

2020 ◽  
Vol 56 (5) ◽  
pp. 562-571
Author(s):  
I. V. Kukushkina ◽  
P. A. Makhnovskii ◽  
L. N. Nefedova ◽  
P. A. Milyaeva ◽  
I. V. Kuzmin ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Gypsy Retrotransposon

scholarly journals Dominant effects of suppressor of Hairy-wing mutations on gypsy-induced alleles of forked and cut in Drosophila melanogaster.

Genetics ◽  
1992 ◽  
Vol 132 (3) ◽  
pp. 691-697 ◽  
Author(s):  
K K Hoover ◽  
T I Gerasimova ◽  
A J Chien ◽  
V G Corces
Keyword(s):  
Drosophila Melanogaster ◽  
Molecular Analysis ◽  
Mutant Phenotype ◽  
Gypsy Element ◽  
Gypsy Retrotransposon

Abstract Mutations induced by the gypsy retrotransposon in the forked (f) and cut (ct) loci render their expression under the control of the suppressor of Hairy-wing [su(Hw)] gene. This action is usually recessive, but su(Hw) acts as a dominant on the alleles fk, ctk and ctMRpN30. Molecular analysis of the gypsy element present in fk indicates that this allele is caused by the insertion of a modified gypsy in which the region normally containing twelve copies of the octamer-like repeat that interacts with the su(Hw) product is altered. Analysis of the gypsy element responsible for the ctk and ctMRpN30 mutations also reveals a correlation between the dominant action of su(Hw) and disruption of the octamer region. We propose that these disruptions alter the affinity and interaction of su(Hw) protein with gypsy DNA, thereby sensitizing the mutant phenotype to fluctuations in su(Hw) product.

scholarly journals Retroviruses in invertebrates: the gypsy retrotransposon is apparently an infectious retrovirus of Drosophila melanogaster.

1994 ◽  
Vol 91 (4) ◽  
pp. 1285-1289 ◽  
Author(s):  
A. Kim ◽  
C. Terzian ◽  
P. Santamaria ◽  
A. Pelisson ◽  
N. Purd'homme ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Gypsy Retrotransposon

Mapping of the gypsy retrotransposon sequence is responsible for the EAST-dependent repression in the yellow gene model system of Drosophila melanogaster

2017 ◽  
Vol 53 (9) ◽  
pp. 988-997 ◽  
Author(s):  
L. S. Melnikova ◽  
M. V. Kostyuchenko ◽  
I. V. Krivega ◽  
I. S. Shapovalov ◽  
P. G. Georgiev ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Model System ◽  
Yellow Gene ◽  
Gene Model ◽  
Retrotransposon Sequence ◽  
Gypsy Retrotransposon

scholarly journals hobo Induced Rearrangements in the yellow Locus Influence the Insulation Effect of the gypsy su(Hw)-Binding Region in Drosophila melanogaster

Genetics ◽  
1998 ◽  
Vol 149 (3) ◽  
pp. 1393-1405 ◽  
Author(s):  
Maria Gause ◽  
Hayk Hovhannisyan ◽  
Tatiana Kan ◽  
Steffi Kuhfittig ◽  
Vladic Mogila ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Regulatory Region ◽  
The Body ◽  
Binding Region ◽  
Sequence Context ◽  
Transcriptional Enhancers ◽  
Insulation Effect ◽  
Enhancer Function ◽  
Gypsy Retrotransposon ◽  
Insight Into

Abstract The su(Hw) protein is responsible for the insulation mediated by the su(Hw)-binding region present in the gypsy retrotransposon. In the y2 mutant, su(Hw) protein partially inhibits yellow transcription by repressing the function of transcriptional enhancers located distally from the yellow promoter with respect to gypsy. y2 mutation derivatives have been induced by the insertion of two hobo copies on the both sides of gypsy: into the yellow intron and into the 5′ regulatory region upstream of the wing and body enhancers. The hobo elements have the same structure and orientation, opposite to the direction of yellow transcription. In the sequence context, where two copies of hobo are separated by the su(Hw)-binding region, hobo-dependent rearrangements are frequently associated with duplications of the region between the hobo elements. Duplication of the su(Hw)-binding region strongly inhibits the insulation of the yellow promoter separated from the body and wing enhancers by gypsy. These results provide a better insight into mechanisms by which the su(Hw)-binding region affects the enhancer function.

scholarly journals The gypsy Insulators Flanking yellow Enhancers Do Not Form a Separate Transcriptional Domain in Drosophila melanogaster: The Enhancers Can Activate an Isolated yellow Promoter

Genetics ◽  
2002 ◽  
Vol 160 (4) ◽  
pp. 1549-1560 ◽  
Author(s):  
Larisa Melnikova ◽  
Maria Gause ◽  
Pavel Georgiev
Keyword(s):  
Drosophila Melanogaster ◽  
Regulatory Region ◽  
Binding Region ◽  
Gypsy Element ◽  
Gypsy Insulator ◽  
Chromosome Addition ◽  
Transcriptional Enhancers ◽  
Blocking Activity ◽  
Enhancer Blocking ◽  
Gypsy Retrotransposon

Abstract The best-characterized insulator in Drosophila melanogaster is the Su(Hw)-binding region contained within the gypsy retrotransposon. In the y2 mutant, Su(Hw) protein partially inhibits yellow transcription by blocking the function of transcriptional enhancers located distally from the yellow promoter with respect to gypsy. Previously we have shown that yellow enhancers can overcome inhibition by a downstream insulator in the yrh1 allele, when a second gypsy element is located upstream of the enhancers. To understand how two insulators neutralize each other, we isolated various deletions that terminate in the regulatory region of the yrh1 allele. To generate these alleles we used DNA elongation by gene conversion of the truncated chromosomes at the end of the yellow regulatory region. We found that gypsy insulator can function at the end of the truncated chromosome. Addition of the gypsy insulator upstream of the yellow enhancers overcomes the enhancer-blocking activity of the gypsy insulator inserted between the yellow enhancers and promoter. These results suggest that the gypsy insulators do not form separate transcriptional domains that delimit the interactions between enhancers and promoters.

scholarly journals Production of Small Noncoding RNAs from the flamenco Locus Is Regulated by the gypsy Retrotransposon of Drosophila melanogaster

Genetics ◽  
2016 ◽  
Vol 204 (2) ◽  
pp. 631-644 ◽  
Author(s):  
V. Guida ◽  
F. M. Cernilogar ◽  
A. Filograna ◽  
R. De Gregorio ◽  
H. Ishizu ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Noncoding Rnas ◽  
Small Noncoding Rnas ◽  
Gypsy Retrotransposon

scholarly journals Structure of Drosophila melanogaster ARC1 reveals a repurposed molecule with characteristics of retroviral Gag

2020 ◽  
Vol 6 (1) ◽  
pp. eaay6354 ◽  
Author(s):  
Matthew A. Cottee ◽  
Suzanne C. Letham ◽  
George R. Young ◽  
Jonathan P. Stoye ◽  
Ian A. Taylor
Keyword(s):  
Drosophila Melanogaster ◽  
Self Assembly ◽  
Neuronal Development ◽  
Particle Assembly ◽  
Neuronal Protein ◽  
Structural Divergence ◽  
Carboxyl Terminal ◽  
Gypsy Retrotransposon ◽  
The Relationship ◽  
Partner Proteins

The tetrapod neuronal protein ARC and its Drosophila melanogaster homolog, dARC1, have important but differing roles in neuronal development. Both are thought to originate through exaptation of ancient Ty3/Gypsy retrotransposon Gag, with their novel function relying on an original capacity for self-assembly and encapsidation of nucleic acids. Here, we present the crystal structure of dARC1 CA and examine the relationship between dARC1, mammalian ARC, and the CA protein of circulating retroviruses. We show that while the overall architecture is highly related to that of orthoretroviral and spumaretroviral CA, there are substantial deviations in both amino- and carboxyl-terminal domains, potentially affecting recruitment of partner proteins and particle assembly. The degree of sequence and structural divergence suggests that Ty3/Gypsy Gag has been exapted on two separate occasions and that, although mammalian ARC and dARC1 share functional similarity, the structures have undergone different adaptations after appropriation into the tetrapod and insect genomes.

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