Acyclic nucleoside phosphonates and human telomerase inhibition

2005 ◽  
Author(s):  
Miroslav Hájek ◽  
Naděžda Matulová ◽  
Ivan Votruba ◽  
Antonín Holý ◽  
Eva Tloušťová
Keyword(s):  
Telomerase Inhibition ◽  
Acyclic Nucleoside Phosphonates ◽  
Acyclic Nucleoside ◽  
Human Telomerase

Inhibition of human telomerase by diphosphates of acyclic nucleoside phosphonates

2005 ◽  
Vol 70 (6) ◽  
pp. 894-900 ◽  
Author(s):  
Miroslav Hájek ◽  
Naděžda Matulová ◽  
Ivan Votruba ◽  
Antonín Holý ◽  
Eva Tloušt’ová
Keyword(s):  
Acyclic Nucleoside Phosphonates ◽  
Acyclic Nucleoside ◽  
Human Telomerase

Tyrosine-based prodrugs of acyclic nucleoside phosphonates

2014 ◽  
Author(s):  
Tomáš Tichý ◽  
Karel Pomeisl ◽  
Marcela Krečmerová ◽  
Charles E. McKenna
Keyword(s):  
Acyclic Nucleoside Phosphonates ◽  
Acyclic Nucleoside

scholarly journals In-silico designing of acyclic nucleoside phosphonates and their anti-HIV potential

2012 ◽  
Vol 12 (Suppl 1) ◽  
pp. P3
Author(s):  
Dipti Yadav ◽  
Anuradha Singh ◽  
Madhu Yadav ◽  
Ramendra K Singh
Keyword(s):  
In Silico ◽  
Acyclic Nucleoside Phosphonates ◽  
Acyclic Nucleoside ◽  
Anti Hiv

scholarly journals Synthesis of fluorinated acyclic nucleoside phosphonates with 5-azacytosine base moiety

Tetrahedron ◽  
2019 ◽  
Vol 75 (39) ◽  
pp. 130529 ◽  
Author(s):  
Karel Pomeisl ◽  
Marcela Krečmerová ◽  
Radek Pohl ◽  
Robert Snoeck ◽  
Graciela Andrei
Keyword(s):  
Acyclic Nucleoside Phosphonates ◽  
Acyclic Nucleoside ◽  
Base Moiety

scholarly journals Activities of Acyclic Nucleoside Phosphonates against Orf Virus in Human and Ovine Cell Monolayers and Organotypic Ovine Raft Cultures

2005 ◽  
Vol 49 (12) ◽  
pp. 4843-4852 ◽  
Author(s):  
F. Dal Pozzo ◽  
G. Andrei ◽  
A. Holý ◽  
J. Van Den Oord ◽  
A. Scagliarini ◽  
...  
Keyword(s):  
Orf Virus ◽  
Human Embryonic Lung ◽  
Raft Culture ◽  
Partial Protection ◽  
Cell Monolayers ◽  
Hel Cells ◽  
Acyclic Nucleoside Phosphonates ◽  
Acyclic Nucleoside ◽  
Virus Strains ◽  
Full Protection

ABSTRACT Orf virus, a member of the Parapoxvirus genus, causes a contagious pustular dermatitis in sheep, goats, and humans. Previous studies have demonstrated the activity of (S)-1-[3-hydroxy-2-(phosphonomethoxy)propyl]cytosine (HPMPC; cidofovir; Vistide) against orf virus in cell culture and humans. We have evaluated a broad range of acyclic nucleoside phosphonates (ANPs) against several orf virus strains in primary lamb keratinocytes (PLKs) and human embryonic lung (HEL) monolayers. HPMPC, (S)-9-[3-hydroxy-2-(phosphonomethoxy)propyl]-2,6- diaminopurine (HPMPDAP), and (R)-9-[3-hydroxy-2-(phosphonomethoxy)propoxy]-2,4-diaminopyrimidine (HPMPO-DAPy) were three of the most active compounds that were subsequently tested in a virus yield assay with PLK and HEL cells by virus titration and DNA quantification. HPMPC, HPMPDAP, and HPMPO-DAPy were evaluated for their activities against orf virus replication in organotypic epithelial raft cultures from differentiated PLK cells. At the highest concentrations (50 and 20 μg/ml), full protection was provided by the three drugs, while at 5 μg/ml, only HPMPDAP and HPMPC offered partial protection. The activities of the three compounds in the raft culture system were confirmed by quantification of infectious virus and viral DNA. These findings provide a rationale for the use of HPMPC and other ANPs in the treatment of orf (contagious ecthyma) in humans and animals.

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