Anti-proteasome autoantibodies contribute to anti-nuclear antibody patterns on human larynx carcinoma cells

The combination of hydroxocobalamin (vitamin B12b) and ascorbicacid (vitamin C) can cause the death of tumor cells at the concentrationsof the components at which they are nontoxic when administeredseparately. This cytotoxic action on epidermoid human larynx carcinomacells HEp-2 in vitro is shown to be due to the hydrogen peroxidegenerated by the combination of vitamins B12b and C. The drop inthe glutathione level preceding cell death was found to be the result ofcombined action of the vitamins. It is supposed that the induction of celldeath by combined action of vitamins B12b and C is connected to the damageof the cell redox system.

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Bioorthogonal metabolic glycoengineering of human larynx carcinoma (HEp-2) cells targeting sialic acid

Beilstein Journal of Organic Chemistry ◽

10.3762/bjoc.6.24 ◽

2010 ◽

Vol 6 ◽

Cited By ~ 16

Author(s):

Arne Homann ◽

Riaz-ul Qamar ◽

Sevnur Serim ◽

Petra Dersch ◽

Jürgen Seibel

Keyword(s):

Sialic Acid ◽

Cell Surface ◽

Carcinoma Cell ◽

Larynx Carcinoma ◽

Interaction Processes ◽

Acid Analogue ◽

Human Larynx ◽

Sialic Acid Analogue ◽

Metabolic Glycoengineering ◽

Human Larynx Carcinoma

Sialic acids are located at the termini of mammalian cell-surface glycostructures, which participate in essential interaction processes including adhesion of pathogens prior to infection and immunogenicity. Here we present the synthesis and bioorthogonal metabolic incorporation of the sialic acid analogue N-(1-oxohex-5-ynyl)neuraminic acid (Neu5Hex) into the cell-surface glycocalyx of a human larynx carcinoma cell line (HEp-2) and its fluorescence labelling by click chemistry.

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4-Methoxycinnamaldehyde inhibited human respiratory syncytial virus in a human larynx carcinoma cell line

Phytomedicine ◽

10.1016/j.phymed.2009.02.016 ◽

2009 ◽

Vol 16 (9) ◽

pp. 882-886 ◽

Cited By ~ 19

Author(s):

Kuo Chih Wang ◽

Jung San Chang ◽

Lien Chai Chiang ◽

Chun Ching Lin

Keyword(s):

Respiratory Syncytial Virus ◽

Cell Line ◽

Carcinoma Cell ◽

Human Respiratory Syncytial Virus ◽

Carcinoma Cell Line ◽

Larynx Carcinoma ◽

Human Larynx ◽

Syncytial Virus ◽

Human Larynx Carcinoma

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Evaluation of Transfected HEP-2 Cell Line Using ß-Galactosidase Reporter Assay System

Baghdad Science Journal ◽

10.21123/bsj.8.3.728-735 ◽

2011 ◽

Vol 8 (3) ◽

pp. 728-735

Author(s):

Baghdad Science Journal

Keyword(s):

Cell Line ◽

High Efficiency ◽

Carcinoma Cell ◽

Transfection Efficiency ◽

Carcinoma Cell Line ◽

Larynx Carcinoma ◽

Human Larynx ◽

Therapy Studies ◽

Human Larynx Carcinoma ◽

Transfection Agent

Liposome-mediated transfection of cancer cells provide a valuable experimental technique to study cellular gene expression and may also be adapted for gene therapy studies. However, the widely recognized advantage of liposome-mediated transfection is high efficiency. Therefore, this study were performed to optimize transfection techniques in human larynx carcinoma cell line Hep-2 using the commercial synthetic lipid TransFast™ Reagent and monitoring the expression efficiency by using the pSV-?-galactosidase Control Vector which encoded ?-galactosidase, maximum transfection efficiency were achieved with TransFast™ Reagent used at the Charge ratios of 2:1 and 0.5 µg DNA/ml, this is indicate that TransFast™ Reagent can be used as an efficient transfection agent to deliver foreign DNA into human larynx carcinoma cell line Hep-2 and expression of the transgene efficiently.

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